Makale Koleksiyonu
Article Colleciton
https://hdl.handle.net/11491/3168
2024-03-28T15:42:43Z
2024-03-28T15:42:43Z
Characterization of p(PmO), p(LO) and p(RO) organoparticles, their bioactivity properties and their effect on pancreatic cancer Capan-1 cell
Alpaslan, Duygu
Turan, Abdullah
Ersen Dudu, Tuba
Moran Bozer, Büşra
Aktaş, Nahit
Türk, Mustafa
https://hdl.handle.net/11491/8777
2024-02-01T07:57:38Z
2023-01-01T00:00:00Z
Characterization of p(PmO), p(LO) and p(RO) organoparticles, their bioactivity properties and their effect on pancreatic cancer Capan-1 cell
Alpaslan, Duygu; Turan, Abdullah; Ersen Dudu, Tuba; Moran Bozer, Büşra; Aktaş, Nahit; Türk, Mustafa
For the first time in the literature, p (PmO), p (LO) and p (RO) organo-particles were synthesized from Peppermint oil, Lemon oil and Rose oil. Of the organo-particles L-929 cell line viability/cytotoxicity and anticancer effect against Capan-1 pancreatic cancer cell line were investigated. p (PmO), p (LO) and p (RO) organo-particles were featured by thermogravimetry (TGA), Fourier Transform Infrared Spectroscopy (FTIR), Scanning electron microscope (SEM), Particle size (DLS), and particle charge (zeta potential, Zeta) analyses. Antioxidant, biocompatible, and antimicrobial activities and in vitro cytotoxicity specialties were investigated. In studies on Capan-1 and L-929 cell lines, it was observed that p (PmO), p (LO) and p (RO) organo-particles were effective on L-929 fibroblast cell line on Capan-1 pancreatic cancer cell line. In addition, it was observed that p (PmO), p (LO) and p (RO) organo-particles were not toxic in L-929 cell lines at high doses. When the Capan-1 cell line MTT analysis results of p (PmO), p (LO) and p (RO) organo-particles were examined, a difference was observed between cell viability rates and apoptosis and necrosis values. The highest % apoptosis rate was observed in the p (RO) organo particle.
2023-01-01T00:00:00Z
Resorbable membrane design: In vitro characterization of silver doped-hydroxyapatite-reinforced XG/PEI semi-IPN composite
Doğan, Deniz
Erdem, Ümit
Moran Bozer, Büşra
Türköz, Mustafa Burak
Yıldırım, Gürcan
Metin, Ayşegül Ülkü
https://hdl.handle.net/11491/8776
2024-02-01T07:27:28Z
2023-01-01T00:00:00Z
Resorbable membrane design: In vitro characterization of silver doped-hydroxyapatite-reinforced XG/PEI semi-IPN composite
Doğan, Deniz; Erdem, Ümit; Moran Bozer, Büşra; Türköz, Mustafa Burak; Yıldırım, Gürcan; Metin, Ayşegül Ülkü
In this study, the production and characterization of silver-doped hydroxyapatite (AgHA) reinforced Xanthan gum (XG) and Polyethyleneimine (PEI) reinforced semi-interpenetrating polymer network (IPN) biocomposite, known to be used as bone cover material for therapeutic purposes in bone tissue, were performed. XG/PEI IPN films containing 2AgHA nanoparticles were produced by simultaneous condensation and ionic gelation. Characteristics of 2AgHA-XG/PEI nanocomposite film were evaluated by structural, morphological (SEM, XRD, FT-IR, TGA, TM, and Raman) and biological activity analysis (degradation, MTT, genotoxicity, and antimicrobial activity) techniques. In the physicochemical characterization, it was determined that 2AgHA nanoparticles were homogeneously dispersed in the XG/PEI-IPN membrane at high concentration and the thermal and mechanical stability of the formed film were high. The nanocomposites showed high antibacterial activity against Acinetobacter Baumannii (A.Baumannii), Staphylococcus aureus (S.aureus), and Streptococcus mutans (S.mutans). L929 exhibited good biocompatibility for fibroblast cells and was determined to support the formation of MCC cells. It was shown that a resorbable 2AgHA-XG/PEI composite material was obtained with a high degradation rate and 64% loss of mass at the end of the 7th day. Physico-chemically developed biocompatible and biodegradable XG-2AgHA/PEI nanocomposite semi-IPN films possessed an important potential for the treatment of defects in bone tissue as an easily applicable bone cover. Besides, it was noted that 2AgHA-XG/PEI biocomposite could increase cell viability, especially in dental-bone treatments for coating, filling, and occlusion.
2023-01-01T00:00:00Z
Photocrosslinked methacrylated pectin and methacrylated hyaluronic acıd wound dressing loaded with oleuropein as bioactive agent
Moran Bozer, Büşra
Özkahraman, Bengi
Mert Balaban, Hümeyra
https://hdl.handle.net/11491/8775
2024-02-01T07:04:52Z
2023-01-01T00:00:00Z
Photocrosslinked methacrylated pectin and methacrylated hyaluronic acıd wound dressing loaded with oleuropein as bioactive agent
Moran Bozer, Büşra; Özkahraman, Bengi; Mert Balaban, Hümeyra
Oleuropein-loaded photocrosslinked hydrogels, consisting of methacrylated pectin (PEC-MA) andmethacrylated hyaluronic acid (HA-MA), were developed as a new hydrogel wound dressing.Pectin and hyaluronic acid were methacrylated and the composite hydrogels were developed byphotocrosslinking of the methacrylated polymer precursors. Methacrylation and the photocros-slinking reactions were approved by1H-NMR and FTIR analyses. In addition to thermal and mor-phological characterizations, swelling andin vitrodegradation studies were performed. Moreimportantly, bioactivity studies, such as cytotoxicity, genotoxicity and hemolysis were performedto reveal the potential of the oleuropein-loaded photocrosslinked hybrid hydrogels for wounddressing applications.In vitroscratch assay studies showed that oleuropein-loaded wound dressingeffectively promoted the wound healing compared to the control in 24 hrs. Oleuropein-loadedphotocrosslinked hydrogels had no genotoxic and cytotoxic effects on L929 cells and could havea potential for wound healing applications.
2023-01-01T00:00:00Z
Synthesis of Silver Nanoparticles From Camellia sinensis Waste and Evaluation of Their Cytotoxic Effect
Erenler, Ramazan
Tunay, Karan
Moran Bozer, Büşra
https://hdl.handle.net/11491/8774
2024-02-01T12:14:12Z
2023-01-01T00:00:00Z
Synthesis of Silver Nanoparticles From Camellia sinensis Waste and Evaluation of Their Cytotoxic Effect
Erenler, Ramazan; Tunay, Karan; Moran Bozer, Büşra
Silver nanoparticle synthesis was achieved using waste tea produced from tea factories and investigated their anticancer activity. The waste material was heated with deionized water at 45°C and then the solution was filtrated. The filtrate was reacted with silver nitrate to yield the silver nanoparticles (AgNPs). The structure of AgNPs was determined by spectroscopic analysis. The cytotoxic effect of extract and AgNPs were performed using the human lung carcinoma cell lines (A549), colon adenocarcinoma cell lines (DLD-1), and mouse fibroblasts (L929) cell lines by MTT assay. In UV-Vis measurement, the maximum absorption was observed at 445 nm. The nanoparticles morphology was established by Transmission electron microscopy (TEM) and particle size was determined as 17.6 nm. The structure of AgNPs was presented as a face-centered cubic structure by X-ray Diffraction (XRD) analysis. The high negative value of zeta potential (-31.3 mV) displayed the stability of the nanostructure. The yield of AgNPs was calculated by elemental analysis as 72.35%. AgNPs exhibited an outstanding effect on A549 and DLD-1 cell lines at 1.0 mg/mL with the lethal effect of 89.3% and 83% respectively. AgNPs synthesized from waste tea may be promising agents for cancer treatment. Since silver nanoparticles synthesized from waste tea revealed excellent cytotoxic effects against various cancer cells. © 2023 Gheorghe Asachi Technical University of Iasi, Romania. All rights reserved.
2023-01-01T00:00:00Z