Tıbbi Hizmetler ve Teknikler Bölümü
Department of Medical Services and Techniques
https://hdl.handle.net/11491/3438
2024-03-28T20:31:02Z
2024-03-28T20:31:02Z
An In Vitro Study on the Cytotoxicity and Genotoxicity of Silver Sulfide Quantum Dots Coated with Meso-2,3-dimercaptosuccinic Acid
Özkan Vardar, Deniz
Aydın, Sevtap
Hocaoğlu, İbrahim
Yağcı Acar, Havva Funda
Başaran, Nurşen
https://hdl.handle.net/11491/7589
2024-01-23T10:45:22Z
2019-01-01T00:00:00Z
An In Vitro Study on the Cytotoxicity and Genotoxicity of Silver Sulfide Quantum Dots Coated with Meso-2,3-dimercaptosuccinic Acid
Özkan Vardar, Deniz; Aydın, Sevtap; Hocaoğlu, İbrahim; Yağcı Acar, Havva Funda; Başaran, Nurşen
Objectives: Silver sulfide (Ag2 S) quantum dots (QDs) are highly promising nanomaterials in bioimaging systems due to their high activities for both imaging and drug/gene delivery. There is insufficient research on the toxicity of Ag2 S QDs coated with meso-2,3-dimercaptosuccinic acid (DMSA). In this study, we aimed to determine the cytotoxicity of Ag2 S QDs coated with DMSA in Chinese hamster lung fibroblast (V79) cells over a wide range of concentrations (5-2000 µg/mL). Materials and Methods: Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assays. The genotoxic and apoptotic effects of DMSA/Ag2 S QDs were also assessed by comet assay and real-time polymerase chain reaction technique, respectively. Results: Cell viability was 54.0±4.8% and 65.7±4.1% at the highest dose (2000 µg/mL) of Ag2 S QDs using the MTT and NRU assays, respectively. Although cell viability decreased above 400 µg/mL (MTT assay) and 800 µg/mL (NRU assay), DNA damage was not induced by DMSA/Ag2 S QDs at the studied concentrations. The mRNA expression levels of p53, caspase-3, caspase-9, Bax, Bcl-2, and survivin genes were altered in the cells exposed to 500 and 1000 µg/mL DMSA/Ag2 S QDs. Conclusion: The cytotoxic effects of DMSA/Ag2 S QDs may occur at high doses through the apoptotic pathways. However, DMSA/Ag2 S QDs appear to be biocompatible at low doses, making them well suited for cell labeling applications.; Amaç: Gümüş sülfür (Ag2 S) kuantum noktaları (QD), hem görüntüleme hem de ilaç/gen hedefleme için büyük aktiviteleri nedeniyle biyo-görüntüleme sisteminde oldukça gelecek vaad eden nanomalzemelerdir. Mezo-2,3-dimerkaptosüksinik asit (DMSA) ile kaplanmış Ag2 S QD’lerin toksisitesi hakkında yeterli çalışma yoktur. Bu çalışmada Çin hamster akciğer fibroblast (V79) hücrelerinde DMSA ile kaplanmış Ag2 S QD’lerin geniş bir konsantrasyon aralığında (5-2000 µg/mL) sitotoksisitesini belirlemeyi amaçladık. Gereç ve Yöntemler: Hücre canlılığı 3-(4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolium bromid (MTT) ve nötral kırmız alım (NRU) deneyleri ile belirlendi. DMSA/Ag2 S QD’lerin genotoksik ve apoptotik etkileri sırasıyla komet analizi ve gerçek zamanlı polimeraz zincir reaksiyonu tekniği ile değerlendirildi. Bulgular: Ag2 S QD’lerin en yüksek dozlarında hücre canlılığı MTT ve NRU deneylerinde sırasıyla 54.0±4.8% ve 65.7±4.1% olarak bulundu. Ancak hücre canlılığı 400 µg/mL (MTT deneyi) ve 800 µg/mL (NRU deney) üzerinde azalmıştır. İncelenen konsantrasyonlarda DNA hasarının DMSA/Ag2 S QD’ler tarafından indüklenmediği belirlenmiştir. P53, kaspaz-3, kaspaz-9, Bax, Bcl-2 ve survivin genlerinin mRNA ekspresyon düzeyleri 500 ve 1000 µg/mL DMSA/Ag2 S QD’lere maruz kalan hücrelerde değişmiştir. Sonuç: DMSA/Ag2 S QD’lerin yüksek dozlarda sitotoksik etkilerinin apoptotik yollarla ortaya çıkabileceği görülmektedir. Bununla birlikte, DMSA/ Ag2 S QD’ler, düşük dozlarda biyolojik olarak uyumlu görünmektedir, bu da onları hücre görüntüleme uygulamaları için uygun kılmaktadır.
2019-01-01T00:00:00Z
In vitro CNS models
Kara, Aslı
Öztürk, Naile
Vural, İmran
https://hdl.handle.net/11491/2106
2021-10-30T20:46:25Z
2017-01-01T00:00:00Z
In vitro CNS models
Kara, Aslı; Öztürk, Naile; Vural, İmran
Gürsoy Özdemir, Yasemin; Şekerdağ, Emine
Endothelial cells form a barrier between the blood and the central nervous system, namely the blood-brain barrier (BBB). Cellular components of the BBB, as well as tight junctions and paracellular barrier characterizations, are described in this chapter. Furthermore, in vitro BBB models are classified as 2D models (monoculture and coculture), 3D, dynamic, and microfluidic models and further explained in detail. The components of the neurovascular unit and their role in obtaining special BBB properties in vitro are outlined, and methods that mimic the dynamic nature of an in vivo microenvironment are discussed. Promising approaches, such as stem cells and microfluidics, for future modeling of the BBB are also mentioned throughout this chapter. As a result it has been concluded that for drug discovery and delivery studies, which target the central nervous system, in vitro BBB models are very useful. © 2017 Elsevier Inc. All rights reserved.
2017-01-01T00:00:00Z
Development of novel self-assembled polymeric micelles from partially hydrolysed poly(2-ethyl-2-oxazoline)-co-PEI-b-PCL block copolymer as non-viral vectors for plasmid DNA in vitro transfection
Kara, Aslı
Öztürk, Naile
Esendağlı, Güneş
Özköse, Umut Uğur
Gülyüz, Sevgi
Yılmaz, Özgür
Telci, Dilek
Bozkır, Asuman
Vural, İmran
https://hdl.handle.net/11491/1856
2021-10-30T20:46:24Z
2018-01-01T00:00:00Z
Development of novel self-assembled polymeric micelles from partially hydrolysed poly(2-ethyl-2-oxazoline)-co-PEI-b-PCL block copolymer as non-viral vectors for plasmid DNA in vitro transfection
Kara, Aslı; Öztürk, Naile; Esendağlı, Güneş; Özköse, Umut Uğur; Gülyüz, Sevgi; Yılmaz, Özgür; Telci, Dilek; Bozkır, Asuman; Vural, İmran
A new efficient, non-viral gene delivery cationic polymeric micellar system was developed by partial hydrolysis of poly(2-ethyl-2-oxazoline) (PEtOx) with two different hydrolysis percentages of PEtOx (30% and 60%) to reduce the disadvantages of the PEI. These self-assemble amphiphilic cationic micelles prepared from poly(2-ethyl-2-oxazoline) 30% -co-poly(ethyleneimine)-block-poly(?-caprolactone) (PEtOx 30% -co-PEI-b-PCL) (PPP30) and poly(2-ethyl-2-oxazoline) 60% -co-poly(ethyleneimine)-block-poly(?-caprolactone) (PEtOx 60% -co-PEI-b-PCL) (PPP60) block copolymers were successfully condensed with pEGFP-C3 plasmid DNA via electrostatic interactions to form micelle/DNA complexes with desirable particle sizes. All formulations showed low critical micelle concentration (CMC) values that means highly stable in serum containing medium. Polymeric micelles were also evaluated for their stability in the presence of serum and nuclease as well as cytotoxicity and transfection efficiency. All our results proved that our novel polymeric micellar system prepared by PPP60 block copolymer offer to be an efficient promising carrier for gene delivery applications. Moreover, these findings contribute to design and development of novel gene vectors with tunable and functionality features and also to reduce the cytotoxicity of PEI by partial hydrolysis of PEtOx an alternative synthesis method to produce linear PEI. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
2018-01-01T00:00:00Z
Necrotic, apoptotic and autophagic cell fates triggered by nanoparticles
Mohammadinejad, Reza
Moosavi, Mohammad Amin
Tavakol, Shima
Özkan Vardar, Deniz
Hosseini, Asieh
Rahmati, Marveh
Dini, Luciana
Hussain, Salik
Mandegary, Ali
Klionsky, Daniel J.
https://hdl.handle.net/11491/1610
2021-10-30T20:46:25Z
2019-01-01T00:00:00Z
Necrotic, apoptotic and autophagic cell fates triggered by nanoparticles
Mohammadinejad, Reza; Moosavi, Mohammad Amin; Tavakol, Shima; Özkan Vardar, Deniz; Hosseini, Asieh; Rahmati, Marveh; Dini, Luciana; Hussain, Salik; Mandegary, Ali; Klionsky, Daniel J.
Nanomaterials have gained a rapid increase in use in a variety of applications that pertain to many aspects of human life. The majority of these innovations are centered on medical applications and a range of industrial and environmental uses ranging from electronics to environmental remediation. Despite the advantages of NPs, the knowledge of their toxicological behavior and their interactions with the cellular machinery that determines cell fate is extremely limited. This review is an attempt to summarize and increase our understanding of the mechanistic basis of nanomaterial interactions with the cellular machinery that governs cell fate and activity. We review the mechanisms of NP-induced necrosis, apoptosis and autophagy and potential implications of these pathways in nanomaterial-induced outcomes. Abbreviations: Ag, silver; CdTe, cadmium telluride; CNTs, carbon nanotubes; EC, endothelial cell; GFP, green fluorescent protein; GO, graphene oxide; GSH, glutathione; HUVECs, human umbilical vein endothelial cells; NP, nanoparticle; PEI, polyethylenimine; PVP, polyvinylpyrrolidone; QD, quantum dot; ROS, reactive oxygen species; SiO2, silicon dioxide; SPIONs, superparamagnetic iron oxide nanoparticles; SWCNT, single-walled carbon nanotubes; TiO2, titanium dioxide; USPION, ultra-small super paramagnetic iron oxide; ZnO, zinc oxide. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
2019-01-01T00:00:00Z