Makale Koleksiyonu
https://hdl.handle.net/11491/3249
Article Colleciton
2024-03-28T15:32:54Z
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A serosurvey on some Canine Vector-borne Zoonoses (Anaplasma spp., Ehrlichia spp., Borrelia burgdorferi, Dirofilaria immitis and Leishmania spp.) in Osmaniye
https://hdl.handle.net/11491/5731
A serosurvey on some Canine Vector-borne Zoonoses (Anaplasma spp., Ehrlichia spp., Borrelia burgdorferi, Dirofilaria immitis and Leishmania spp.) in Osmaniye
Güven Gökmen, Tülin; Günaydın, Elçin; Turut, Nevin; Akın, Bünyamin; Koç, Özgür; Ütük, Armağan Erdem
Abstract: Vector-borne diseases in dogs are of major global significance for their impact on animal and human health. Especially, it is necessary to determine the prevalence of agents found in reservoir animals by conventional, molecular and serological methods for the application of control programs for these diseases. Serosurvey studies are one of the reliable methods to know the presence and prevalence of these diseases in our country and region. In this study, it was aimed to detect the prevalence of Ehrlichia canis/E.ewingii, Anaplasma platys/A.phagocytophilum, Borrelia burgdorferi, Dirofilaria immitis and Leishmania infantum in dogs in Osmaniye. Five canine vector-borne diseases were investigated with a rapid in-clinic enzyme-linked immunosorbent assay in 100 clinically healthy dog serum samples in Osmaniye city center, Düziçi, Sumbas, Kadirli, Hasanbeyli, Bahçe and Toprakkale districts. Seroprevalence rate was detected as 3% for E.canis/E.ewingii and 1% for D.immitis by SNAP 4Dx PLUS. The prevalence of A.platys/A.phagocytophilum, Borrelia burgdorferi and L.infantum were determined as 0%. In conclusion, our study in which we determined the seroprevalence of dog vector-borne diseases in Osmaniye is the first study in which five agents are determined in one step and will contribute to the effective control programs prepared for animal and public health in our region.; Öz: Köpeklerde bulunan vektör kaynaklı hastalıklar, hayvan ve insan sağlığı üzerindeki etkileri açısından büyük önem taşımaktadır. Özellikle belirli bölgelerde bu hastalıklara yönelik kontrol programlarının uygulanması için, öncelikle rezervuar hayvanlarda bulunan etkenlerin konvansiyonel, moleküler ve serolojik yöntemlerle prevalansının belirlenmesi gereklidir. Serolojik araştırma çalışmaları ülkemizde ve bölgemizde bu hastalıkların varlığını ve yaygınlığını bilmek için güvenilir yöntemlerden biridir. Bu çalışmada, Osmaniye ilinde köpeklerde Ehrlichia canis / E.ewingii, Anaplasma platys / A.phagocytophilum, Borrelia burgdorferi, Dirofilaria immitis ve Leishmania infantum prevalansının belirlenmesi amaçlanmıştır. Osmaniye merkez, Düziçi, Sumbas, Kadirli, Hasanbeyli, Bahçe ve Toprakkale ilçelerinde klinik olarak sağlıklı yüz köpekten alınan serum örneklerinde hızlı bir immünosorbent testi ile beş köpek vektör kaynaklı hastalık araştırılmıştır. SNAP 4Dx PLUS testi ile seroprevalans oranı E.canis / E.ewingii için %3, D.immitis için %1 olarak tespit edilmiştir. A.platys / A.phagocytophilum, Borrelia burgdorferi ve L.infantum prevalansı ise % 0 olarak belirlenmiştir. Sonuç olarak, Osmaniye'de köpek vektör kaynaklı hastalıkların seroprevalanslarını tespit ettiğimiz çalışmamız, bölgede beş etkenin tek aşamada belirlendiği ilk çalışmadır ve bölgemizde hayvan ve halk sağlığı için hazırlanan efektif kontrol programlarına katkı sağlayacaktır
2019-01-01T00:00:00Z
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Effect of chronic exposure to sodium fluoride and 7,12-dimethylbenz[a]anthracene on some blood parameters and hepatic, renal, and cardiac histopathology in rats
https://hdl.handle.net/11491/514
Effect of chronic exposure to sodium fluoride and 7,12-dimethylbenz[a]anthracene on some blood parameters and hepatic, renal, and cardiac histopathology in rats
Yıldırım, Serkan; Ekin, Suat; Huyut, Zübeyir; Oto, Gökhan; Comba, Arzu; Uyar, Hasan; Şengül, Emin; Çınar, D. Ali
This study aimed to investigate the effects of both sodium fluoride (NaF) and 7,12-dimethylbenz[a]anthracene (DMBA), both separately and in combination, on some blood parameters and hepatic, renal, and cardiac histopathology in rats. Forty male Wistar albino rats, weighing 250-300 g, were randomly divided into one control and three experimental groups (i) a NaF group who received 15 ppm of NaF in their drinking water for 90 days, (ii) a DMBA group who received 10 mg DMBA/kg body weight/po/ weekly for 90 days, and (iii) a NaF+DMBA group who received 15 ppm NaF in their drinking water plus 10 mg DMBA/kg bw/po/weekly for 90 days. The animals in the groups were sacrificed at the end of the 90 days. The AST, ALT, LDH, CK, creatinine, troponin I, and MDA levels increased in the NaF, DMBA, and NaF+DMBA groups compared to the control group, while the WBC, K, Na, Cl, urea, SOD, GSH-Px, CAT, and GSH values showed a statistically significant decrease (p<0.05). In addition, the CK-MB significantly increased in the DMBA and NaF+DMBA groups compared to the control group (p<0.05). The histological structure of the liver, kidney, and heart tissues in the control group was normal. In the NaF and DMBA groups, degenerative and necrotic changes were detected. In the NaF+DMBA group: (i) the liver exhibited hydropic degeneration and coagulation necrosis in hepatocytes, severe dilation in the sinusoids, congestion in the central and portal regions, and mononuclear cell infiltration in the portal region; (ii) the kidneys displayed congestion in the glomerulus and interstitial vessels, interstitial nephritis, diffuse hydropic degeneration, and coagulation necrosis in the tubule epithelium; (iii) the heart showed myocardial hyperemia, severe mononuclear cell infiltration in interstitial tissue, hyaline degeneration, and Zenker’s necrosis in myocardium As a result of these blood and oxidative stress parameters and histopathological findings, it was determined that NaF, DMBA, and NaF+DMBA induce toxicity in the liver, kidney, and heart tissues and thus play an important role in the physiopathology of toxicity. © 2018, ISFR.
2018-01-01T00:00:00Z
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How long-term intake of sodium fluoride (NAF) in different doses and 7,12 dimethylbenz(A)anhtracene (DMBA) affect the erythrocyte parameters in rats?
https://hdl.handle.net/11491/171
How long-term intake of sodium fluoride (NAF) in different doses and 7,12 dimethylbenz(A)anhtracene (DMBA) affect the erythrocyte parameters in rats?
Comba, Bahat; Oto, Gökhan; Arıhan, Okan; Comba, Arzu; Uyar, Hasan
This study was aimed to search the effect of Sodium Fluoride (NaF) and 7,12 dimethylbenz(a)anthracene (DMBA) to erythrocyte fragility and parameters in rats. The nine groups were formed and each group contained 8 animals. Group 1: Control group (without any treatment). Group 2: Sesame oil (vehicle for DMBA). Group 3: 1 ppm NaF. Group 4: 15 ppm NaF. Group 5: 30 ppm NaF. Group 6: DMBA.Group 7: 1 ppm NaF + DMBA. Group 8: 15 ppm NaF + DMBA. Group 9: 30 ppm NaF + DMBA. Fluoride was added into the animals’ drinking water in the form of NaF once a day, for 12 weeks. DMBA (10 mg/kg) was administered once a week and in a total of 12 weeks with oral gavage. Erythrocyte fragility was analyzed with osmotic hemolysis method and erythrocyte parameters with blood cell counter in whole blood. At 0.4% NaCl concentration groups 4, 5, 6, 8, and 9 showed significantly higher erythrocyte fragility values than control group (p≤0.05). At 0.5% NaCl concentration, groups 6, 8 and 9 showed significant increase in erythrocyte fragility compared to other groups (p≤0.05). The erythrocyte and hematocrit values were found significantly high in group 5 (p≤0.001) and group 4 (p≤0.01) while it was found low in all groups with DMBA (6, 7, 8, 9) (p≤0.05) compared to control group. Hemoglobin amount in group 5(p≤0.01) and group 4 (p≤0.05) were significantly higher than other groups. MCV and MCH in group 5 were significantly lower and these values in all groups with DMBA (6, 7, 8, 9) were determined significantly high compared to other groups. RDWC in group 5 (p≤0.001) and group 4 (p≤0.01) and in all groups with DMBA (6, 7, 8, 9) (p≤0.05) was significantly increased compared to other groups. As a result, exposure to high doses of floride and DMBA may cause augmented erythrocyte fragility, abnormal erythrocyte parameters and anemia. Therefore, measures must be taken to protect the health of all living organisms in area exposed to high levels of fluoride and DMBA.
2019-01-01T00:00:00Z
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Some mineral substance, oxidative stress and total antioxidant levels in Norduz and Morkaraman sheep
https://hdl.handle.net/11491/170
Some mineral substance, oxidative stress and total antioxidant levels in Norduz and Morkaraman sheep
Mis, Leyla; Mert, Handan; Comba, Arzu; Comba, Bahat; Doğan Söğütlü, İnci; Irak, Kıvanç; Mert, Nihat
The aim of the study was to compare some mineral substances and total antioxidant and totaloxidan levels of healthy Morkaraman and Norduz sheep breeds in the same age and care conditions. A total of 20 animals, 10 from each sheep breed, were included in the study. Serum total oxidant status (TOS) and total antioxidant status (TAS) were determined by a colorimetric method. OSI value was determined by calculating the TASTOS ratio. When the two sheep were compared, there was a difference between TAS, Mg, P levels. Antioxidant levels of Norduz sheep were higher than morkaraman sheep. The given data may be the reference value for studies of these sheep breeds; Sunulan çalışmanın amacı, aynı yaş ve bakım şartlarında sağlıklı Morkaraman ve Norduz koyun ırklarının bazı mineral madde ve total antioksidan, total oksidan düzeylerinin karşılaştırılmasıdır. Her bir koyun ırkından 10 adet olmak üzere toplam 20 hayvan çalışmaya dahil edildi. Serum toplam oksidan seviye (TOS) ve toplam antioksidan seviye (TAS) kolorimetrik bir yöntemle belirlendi. OSI değeri, TAS-TOS oranı hesaplanarak belirlendi. İki ırk karşılaştırıldığında TAS, Mg, P düzeyleri arasında fark gözlendi. Norduz ırkı koyunların antioksidan düzeyinin daha yüksek olduğu belirlendi. Verilen veriler bu koyun ırklarına ait çalışmalarda referans değer olabilecektir
2018-10-09T00:00:00Z