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dc.contributor.authorShomali-Moghaddam, Naznoosh
dc.contributor.authorGül, Nursel
dc.contributor.authorGökalp Özkorkmaz, Ebru
dc.contributor.authorÖzlük, Aydın
dc.identifier.citationShomali-Moghaddam, N., Gül, N., Gökalp Özkorkmaz, E., Özlük, A. (2018). Lectin histochemistry of Agrotis segetum midgut cells and peritrophic membrane. Gazi University Journal of Science, 31(4), 1022-1031.en_US
dc.description.abstractMidgut cell and peritrophic membranes of grain pest Agrotis segetum larvae were examined by light microscope. Six biotinylated lectins were used in the assays by applying histochemical methods (Avidin-Biotin-Peroxidase). The aim of this study was to find lectins binding on peritrophic membranes if they were available as insecticidal agents. Results of the study indicated that lectins BPA (Bauhinia purpurea) and GS-I (Griffonia simplicifolia) strongly stained whereas, PNA (Pea Nut Agglutinin) and UEA-1 (Ulex europaeus) moderately stained the tissues. However, WGA (Wheat Germ Agglutinin) and Con-A (Canavalia ensiformis) stained the tissues weakly. The common feature of two binding proteins, BPA and GS-I lectin was binding to D-galactose. Our examinations revealed that D-galactose mostly exists in A. segetum larvae midgut cell membranes and peritrophic membrane and this means BPA and GS-I lectins can be used as insecticidal agents against A. segetum. © 2018, Gazi University Eti Mahallesi. All rights reserved.en_US
dc.publisherGazi Universityen_US
dc.subjectAgrotis Segetum Larvaeen_US
dc.subjectBiotinylated Lectinsen_US
dc.subjectMidgut Cellsen_US
dc.subjectPeritrophic Membraneen_US
dc.titleLectin histochemistry of Agrotis segetum midgut cells and peritrophic membraneen_US
dc.relation.journalGazi University Journal of Scienceen_US
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US

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