Nucleotide incorporated magnetic microparticles for isolation of DNA

Abstract

For the comprehensive understanding of many diseases, especially genetic disease, the highly purified deoxyribonucleic acid (DNA) is needed to perform in-detail studies. There are various methods to obtain highly purified DNA, but low in number. In this study, Fe&Ni-mc-poly (2-hydroxyethyl methacrylate-adenine methacrylate) [poly(HEMA-AdeM)] microparticles were synthesized to get high purification ratio because of natural and thus selective interaction between adenine on the microparticles and thymine on the DNA molecule and also high amount of DNA purified. For the characterization of microparticles scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), elemental analysis, swelling ratio and zeta size analysis methods were performed. All experiments were performed batch-wise for the determination of optimum conditions such as pH, initial DNA concentration, temperature and interaction time. At the end of the all study, the desorption yield was obtained as 97.7% and after 5 adsorption-desorption cycle, the adsorption capacity was decreased only 3%. © 2016 Elsevier Ltd