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dc.contributor.authorGöktürk, İlgim
dc.contributor.authorTamahkar Irmak, Emel
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-05-13T09:04:48Z
dc.date.available2019-05-13T09:04:48Z
dc.date.issued2018
dc.identifier.citationGöktürk, I., Tamahkar, E., Yılmaz, F., Denizli, A. (2018). Protein depletion with bacterial cellulose nanofibers. Journal of Chromatography B, 1099, 1-9.en_US
dc.identifier.issn1570-0232
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2018.08.030
dc.identifier.urihttps://hdl.handle.net/11491/1645
dc.description.abstractIn this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications. Molecular imprinting was achieved by using metal ion coordination interactions of N?methacryloyl?(L)?histidinemethylester (MAH) monomer and Cu(II) ions. MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature. The characterization of the MIP-cBCNFs was carried out by FTIR-ATR, SEM, contact angle measurements and surface area measurements. The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system. The selectivity of the MIP-cBCNFs was investigated by using non-template human transferrin (HTR), and myoglobin (Myo). The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively. In addition, the depletion of HSA from artificial human plasma was confirmed by SDS-PAGE and 2-D gel electrophoresis. As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability. © 2018en_US
dc.language.isoeng
dc.publisherElsevier B.V.en_US
dc.relation.isversionof10.1016/j.jchromb.2018.08.030en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacterial Cellulose Nanofibersen_US
dc.subjectMetal Ion Coordinationen_US
dc.subjectMolecularly Imprinted Polymersen_US
dc.subjectProtein Recognitionen_US
dc.subjectProteomicsen_US
dc.titleProtein depletion with bacterial cellulose nanofibersen_US
dc.typearticleen_US
dc.relation.journalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciencesen_US
dc.departmentHitit Üniversitesi, Mühendislik Fakültesi, Kimya Mühendisliği Bölümüen_US
dc.identifier.volume1099en_US
dc.identifier.startpage1en_US
dc.identifier.endpage9en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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