Optimization of separation of several sulfonamides in acetonitrile-water binary mixtures by using symmetry shield RP-8 column

Abstract

In this work, the proportion of organic modifier of the hydro-organic mobile phase was optimized in order to separate twelve compounds namely sulfaguanidine, sulfanilamide, sulfadiazine, sulfathiazole, sulfamethazine, sulfamerazine, sulfamethoxypyridazine, sulfamonomethoxyne, sulfachloropyridazine, sulfadoxine, sulfafurazole and dapson using Symmetry Shield RP-8 Column. Mobile phase was optimized by establishing relationships between retention parameters and Reichardt’s RTN scale of solvent polarity. In addition, selectivity (α) and resolution (RS) values were used in order to establish a general model relating elution behavior of substances with the composition of mobile phase. Logarithm of retention factor corresponding to pure water, log kw, which is the most commonly employed chromatographic hydrophobicity parameter determined by using chromatographic data.