Comparison of the effects of Neostigmine and Sugammadex on Colonic Anastomotic Strength in Rats

Abstract

Background:After colorectal surgery, anastomotic leakage is a major cause of mortality andmorbidity. There are many factors affecting anastomotic leakage. It is known that agentssuch as neostigmine that is used to reverse neuromuscular blockade have certain effectson anastomosis. In our study, in which we planned to test this hypothesis, we aimed tocompare the possible effects of different doses of sugammadex and neostigmine on colonanastomosis strength in a colonic resection anastomosis model in rats.Materials and methods:Forty adult Wistar albino male rats were divided into five groups ascontrol (group C), Sugammadex 16 mg/kg (group SL), sugammadex 96 mg/kg (group SH),neostigmine 0.3mmol/kg (group NL), and neostigmine 1.5mmol/kg (group NH). The trans-verse colons of all rats were resected, and colonic anastomosis was performed. Appropriatedrug doses according to the groups were given on the postoperative seventh day, and tissuehydroxyproline (TH) level and anastomotic bursting pressure were measured.Results:Anastomotic bursting pressure values were statistically significantly different be-tween the groups (P¼0.001). The bursting pressure in group SH was significantly highercompared with group C, group NL, and group NH. The hydroxyproline values were sta-tistically significantly different between the groups (P¼0.015). According to the post hoctest results, the difference was between group SH and group C (P¼0.007). There were nosignificant differences between the other groups (P>0.05). There was no significant dif-ference in terms of intra-abdominal adhesion rates between the groups.Conclusions:In our study, we found that low and high doses of neostigmine had no variableeffect on anastomosis, but high dose of sugammadex (96 mg/kg) had an increasing effecton intestinal anastomosis strength