Magnetic Nanosensor Design and Assay of an Anti-Tuberculosis Drug

Abstract

In the present work, an anti-tuberculosis drug, morphazinamide HCl (MRA) was investigated at ultra-trace level in real sample for the first time. Iron (III) oxide nanoparticles (Fe(2)O(3)NPs) were casted on a glassy carbon electrode (GCE) for the determination of MRA. Cyclic voltammetry, electrochemical impedance spectroscopy, transmission electron microscopy, scanning electron microscopy and energy-dispersive X-ray spectroscopy were employed for morphological characterization of the electrode surface. The results displayed a higher surface area and greater conductivity for the Fe(2)O(3)NPs modified glassy carbon electrode (Fe(2)O(3)NPs/ GCE) than bare GCE. The square wave voltammetry response of the Fe(2)O(3)NPs/GCE was linear with MRA concentration in the range of 1 mu M to 100 mu M under optimized conditions (0.1M phosphate buffer solution at pH 3.0; drop-casting volume 10 mu L; accumulation time 15 s; accumulation potential -0.3 V), with a detection limit of 0.21 nM. Also, the proposed procedure was very efficient for MRA determination from human serum sample with high recoveries. (C) 2019 The Electrochemical Society.