Lipoic Acid Decreases 3-Nitrotyrosine and Cytokine Levels In A Rat Sepsis Model

Abstract

It was aimed to investigate the effects of lipoic acid (LA) on 3-nitrotyrosine (3-NT), interleukin-1? (IL-1?), tumor necrosis factor- ? (TNF-?), magnesium and trace elements levels in serum of rats with sepsis. Control group (n=10) received a single dose of saline, lipopolysaccharide (LPS) group ( n=10) was injected a single dose of 20 mg/kg LPS, and LA+LPS (n=10) group was injected a single dose of 20 mg/kg LPS at the end of third day of 10 mg/kg/day LA injection. The serum 3-NT levels were determined by a high performance liquid chromatography. The serum levels of IL-1? and TNF-? were measured by enzyme linked immunosorbent assay. Determination of magnesium and trace element levels were performed by inductively coupled plasma mass spectromet er. 3-NT, IL-1? and TNF-? levels in control and LA+LPS groups were not determined. However, observable levels of these parameters were detected in the LPS group. It was found that manganese levels were positively correlated with IL-1? and TNF-? in LPS group. LA increased the manganese levels, while it decreased magnesium and iron levels in LA+LPS group. Our results show that LA decreases serum levels of 3-NT, IL-1? and TNF-? in rats with sepsis, and it is also effective on trace element levels. Decreased levels of manganese and its association with IL-1? and TNF-? in the LPS group suggest that manganese may be associated with immune response.