Photocrosslinked methacrylated pectin and methacrylated hyaluronic acıd wound dressing loaded with oleuropein as bioactive agent

Abstract

Oleuropein-loaded photocrosslinked hydrogels, consisting of methacrylated pectin (PEC-MA) andmethacrylated hyaluronic acid (HA-MA), were developed as a new hydrogel wound dressing.Pectin and hyaluronic acid were methacrylated and the composite hydrogels were developed byphotocrosslinking of the methacrylated polymer precursors. Methacrylation and the photocros-slinking reactions were approved by1H-NMR and FTIR analyses. In addition to thermal and mor-phological characterizations, swelling andin vitrodegradation studies were performed. Moreimportantly, bioactivity studies, such as cytotoxicity, genotoxicity and hemolysis were performedto reveal the potential of the oleuropein-loaded photocrosslinked hybrid hydrogels for wounddressing applications.In vitroscratch assay studies showed that oleuropein-loaded wound dressingeffectively promoted the wound healing compared to the control in 24 hrs. Oleuropein-loadedphotocrosslinked hydrogels had no genotoxic and cytotoxic effects on L929 cells and could havea potential for wound healing applications.