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    Effects Of Thymoquinone, Zeolite And Platelet Rich Plasma On The Healing Of Ischemic Colonic Anastomosis
    (2019) Pehlivanlı, Faruk; Karaca, Gökhan; Aydın, Oktay; Altunkaya, Canan; Şahiner, İbrahim Tayfun; Özden, Hüseyin; Pekcici, Mevlüt Recep
    Objective: Anastomosis on ischemic intestines is a problematic issue in daily surgical routine. In this study, we investigated the potential effects of thymoquinone, zeolite and platelet rich plasma. Material and Methods: Fifty rats were divided into five groups. In the sham group all rats underwent colonic anastomosis without any ischemic insult. Colonic anastomosis was performed after mesenteric ischemia was induced by clamping the superior mesenteric artery in other study groups. In the control group, no medication was given to the rats after ishemic anastomosis. In Zeolite group and thymoquinone group, Zeolite and thymoquinone were applied locally on the anastomosis. In the platelet rich plasma group, platelet rich plasma was applied locally on the anastomosis. All the rats were sacrified on the 10th day. Blood and tissue samples were retrieved. Results: Hydroxyproline levels were not different between the groups. Bursting pressure was higher in the thymoquinone group than the control group. Reepithelization was higher than the zeolite group in the sham and thymoquinone groups. TNF-? and IL 1? levels were significantly higher in the sham group than all the other groups. TNF-? and IL 1? levels were also significantly higher in the thymoquinone, zeolite and platelet rich plasma groups than the control group and higher in the thymoquinone group than the zeolite and platelet rich plasma groups. Inflammation and ischemic necrosis levels were not different between the groups. Conclusion: Our data is suggesting that thymoquinone and platelet rich plasma have positive effects on ischemic anastomosis healing, while thymoquinone has the best results.
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    Platelet-Rich Plasma May Offer a New Hope in Suppressed Wound Healing When Compared to Mesenchymal Stem Cells
    (Mdpi, 2018) Aydin, Oktay; Karaca, Gokhan; Pehlivanli, Faruk; Altunkaya, Canan; Uzun, Hafize; Ozden, Huseyin; Guler, Osman
    Background: The present study investigated the effectiveness of platelet-rich plasma (PRP) and mesenchymal stem cells (MSCs) in wound healing suppressed by corticosteroid in rats. Methods: Forty rats were separated into four groups. To disrupt the wound-healing processes, intraperitoneal single dose 10 mg/kg methylprednisolone was administered to all rats with the exception of Sham-S group. Then, full-thickness incision was performed to the abdominal skin of all animals, and PRP or MSCs were applied to the incision line except the Sham-S and Sham-M group animals. Ten days later, all animals were sacrificed to investigate: tissue collagenization, inflammation, and re-epithelialization grades histopathologically; and tissue hydroxyproline (HP), interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha levels biochemically. Results: Collagenization (p = 0.003) and inflammation grade (p = 0.002) values were higher in PR group. Tissue HP level value was found to be high in MC group (p < 0.001). Tissue IL-1 beta level value of Sham-M group was lower than those of other groups (p < 0.001). Conclusions: This preliminary study revealed that PRP could improve the histopathological grades in wound healing which was suppressed by corticosteroid in rats, while MSCs could show their therapeutic effects via biochemical route. These positive effects were more salient in PR group.
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    Öğe
    Progesterone Increases The Ischemic Damage In Male Rats With Cerebral Ischemia Reperfusion Injury
    (2019) Öğden, Mustafa; Bakar, Bülent; Altunkaya, Canan; Dağlı, Ahmet Turan; Özdemir, Alemiddin; Özveren, Mehmet Faik
    Objective: In the current literature, there are few accepted pharmacological treatment methods for acute ischemic stroke. This study was conducted to investigate the effects of progesterone on transient ischemia / reperfusion injury in male rats. Material and Methods: A total of 25 Wistar albino male and young rats were divided into 5 groups called Control group, acute stage groups (Sham-A and PRG-A), and chronic stage groups (Sham-C and PRG-C), randomly and their internal carotid arteries were compressed using temporary aneurysm clips for 30 minutes. At 4 hours after removal of the clips, progesterone was injected to the animals of the PRG-A and PRG-C group via intraperitoneal route. After sacrifice of all animals, pyknotic and necrotic neuronal cells were counted in hippocampal cornu amnonis (CA)1, CA2, CA3 and parietal cortical regions, histopathologically. Tissue interleukin (IL)-6, IL-10, caspase-3, and hypoxia-inducible factor-1 (HIF1) gene expression levels were evaluated using real time polymerase chain reaction assay. Results: Histopathological and biochemical findings revealed that progesterone has no healing effects on ischaemic neuronal tissue damage in either acute or chronic period. Moreover, progesterone was found to significantly increase symptoms of ischaemia in both acute and chronic periods compared to healthy control group and even compared to Sham groups where I/R injury was applied and no experimental agent was administered. Conclusion: At the end of this study, it was thought that progesterone had no therapeutic effect on cerebral ischemia / reperfusion injury in male sex rats and it could lead to increase it further, unfortunately.