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    Effects of Pelargonium sidoides (UMCA ®) on pulmonary contusion from blunt thoracic trauma in rats
    (2020) Doğan, Gül; Doğan, Güvenç; Karaca, Onur; Ayaz, Ercan
    Aim: The aim of the present study was to evaluate the effect of Pelargonium sidoides (UMCA ®) which was antibacterial, antiviral, anti-inflammatory and antioxidant, on pulmonary contusion (PC) caused by isolated blunt thoracic trauma (BTT) in an experimental rat model. Material and Methods: A total of 24 rats were divided into three groups: control group (CG), sham group (SG), and Pelargonium sidoidesgroup (PSG). PC was induced by isolated BTT for all the groups except the control group. Pelargonium sidoidestreatment was performed by gavage for 72 hours to the PSG after truma. Blood and tissue samples were collected from the groups. Malondialdehyde (MDA), superoxide dismutase (SOD),glutathione (GSH) and arterial blood gas parameters were measured. Lung tissue samples were collected for histopathology. Results: Histopathologically, alveolar congestion, hemorrhage,edema,disruption and neutrophil infiltrationwere significantly higher in SG when compared with CG(p<0.001, p=0.007, p=0.040, p=0.003, p=0.001). Leukocyte infiltration was significantly decreased in PSG when compared with SG (p=0.025). Biochemically, MDA level was significantly higher in SG than in CG (p<0.001) and GSH level was significantly lower in SG than in CG (p<0.001). MDA level was significantly lower in PSG than in SG (p=0.002). In blood gas parameters PH and PO2 level was significantly higher in PSG than in SG (p=0.013, p<0.001) and PCO2 level was significantly lower in PSG than in SG (p<0.001). Conclusion: PS prevents further injury by decreasing leukocyte infiltration,MDAand regulating ventilation -perfusion in lung contusions. PS may have a role in the progression of inflammation but not in preventing the pathologic disruption of pulmonary parenchyma exactly.
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    Effects of Pulsed and Sinusoidal Electromagnetic Fields on Rat Testes
    (Sci Printers & Publ Inc, 2020) Tunik, Selcuk; Baran, Ozlem Pamukcu; Akpolat, Veysi; Ayaz, Ercan; Nergiz, Yusuf; Celik, M. Salih; Yumusak, Ozkan
    OBJECTIVE: To investigate the role of 50-Hz extremely low frequency pulsed (PEMF) and sinusoidal (SEMF) electromagnetic fields on the testis using a rat model. STUDY DESIGN: Twenty-seven male Wistar albino rats were used. The rats were divided into three groups (n=9 each): sham group, SEMF group, and PEMF group. The SEMF and PEMF groups (pulse time: 20 milliseconds, pulse frequence: 50 Hz) were subjected to 1.5 mT, 50 Hz exposure 6 hours a day, 5 days a week for 28 days in methacrylate boxes. Formalin-fixed, paraffin-embedded tissue sections were stained with H-E and PAS. In addition, E-cadherin and type IV collagen expressions were examined immunohistochemically. RESULTS: Seminiferous tubule basement membranes decreased with EMFs, especially those treated with SEMF. In addition, expression levels of E-cadherin on seminiferous epithelium decreased in PEMF and SEMF groups. The expression level of type IV collagen was also decreased in perivascular and seminiferous tubule basement membrane as compared to that in the sham group. CONCLUSION: PEMF and SEMF have adverse effects on the testis at a histopathological level. EMF leads to a decrease in E-cadherin and type IV collagen expression levels.
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    The comparison of endogenous angiogenesis inhibitors in normotensive and preeclamptic placentas: An immunohistochemical study
    (Informa Healthcare, 2014) Ayaz, Ercan; Nergiz, Yusuf; Tunik, Selçuk; Yalınkaya, Ahmet
    Objective: Recently, it has been reported that endogenous angiogenesis inhibitors play a key role in the pathophysiology of preeclampsia. Thrombospondin-1, angiostatin and vasostatin are endogenous angiogenesis inhibitors and so far have not been shown in placenta at the immunohistochemical level. The aim of this study was to compare staining patterns of these endogenous angiogenesis inhibitors immunohistochemically in placentas of preeclamptic and normotensive pregnant women. Methods: Into the study, placentas from 20 preeclamptic and 20 normotensive pregnant women were included. Central and peripheral tissues were taken from both sides of placentas. Paraffin tissue blocks were prepared and stained for immunohistochemical analysis. Slides were evaluated for syncytiotrophoblasts, cytotrophoblasts, extra-villous throphoblasts and decidual cells. The degree of staining of slides were classified as negative, weak, moderate and strong. Results: Samples from preeclamptic patients were compared with those of normotensive. Staining of thrombospondin-1 was observed to increase in decidual cells, syncytiotrophoblasts in chorionic and stem villi and stroma of stem villi. Increased staining of thrombospondin-1 was only significant in the amniotic epithelium of the central sections. In addition, increased staining intensity of angiostatin was detected in the amniotic epithelium and chorionic plate of central sections of placenta. In peripheral sections, staining of angiostatin also increased in decidual cells but decreased in chorionic plate. Vasostatin staining in decidual cells, decidual stroma and chorionic villous stroma from peripheral sections decreased, but any difference was not observed in the central sections. Conclusion: Our results suggest that thrombospondin-1, angiostatin and vasostatin may play a role in the pathophysiology of preeclampsia. Further molecular studies are required to understand this role. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.
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    The immunohistochemical and ultrastructural evaluation of pericytes in human full term placentas of gestasyonal diabetes mellitus
    (Carbone Editore di Alessandro Pennino, 2013) Deveci, Engin; Söker, Sevda İpek; Turgut, Abdülkadir; Aktaş, Ayfer; Ayaz, Ercan; Sak, Sibel; Şeker, Uğur
    Pericytes, vessel wall plays a stabilizing role in the regulation of blood flow in the microcirculation. The purpose of this study is based on non-diabetic pregnancies complicated by gestational diabetes pregnancies to investigate the morphological structure of pericyte cells. In this study, as a control group human placental tissues from normotensive pregnancies was collected from diabetic wome at 28-35 weeks of gestation. Pericytes with smooth alpha-actin positive cells, endothelial cells, and painted like a belt was surrounded. Pericytes, capillary plexus and endothelial cells of large vessels in the mesenchyme around the middle shows desmin positive reaction. Placental microvessels examined by transmission electron microscopy showed many pericytes. Placentas of gestational diabetes group, heterochromatin nucleus hypertrophy, dilatation of endoplasmic reticulum, mitochondria cristae in length, shortening was observed thickening of the filamentary structure. The contractile function of the barrier formed by endothelial cells, pericytes and can increase the contractions were considered.