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Öğe ASSESSMENT OF THE WHEATGRASS EFFECTS ON ANTIOXIDANT CAPACITY AND DNA DAMAGE IN RATS WITH EXPERIMENTAL RENAL FAILURE(Parlar Scientific Publications (P S P), 2019) Mis, Leyla; Comba, Bahat; Basbugan, Yildiray; Yasar, Semih; Comba, ArzuIn this study, it was aimed to evaluate the effect of wheatgrass on antioxidant capacity and DNA damage in rats with renal failure. In the study, 200-300 g 24 healthy Wistar-Albino male rats obtained from YYU Experimental Animal Unit were used. The rats were randomly divided into 4 groups and each group was formed with 6 rats. Groups were allocated as; 1. Control Group, 2. Renal Failure Group (Genta Group) 3. Renal Failure + Wheatgrass Group (Genta +Wheatgrass) 4. Wheatgrass Group. Gentamicin sulfate was given intraperitoneal at a dose of 50 mg/kg for 1 week and once a day to the groups with renal failure (Groups 2 and 3). Wheat grass extract (10 ml/kg) was added to the drinking water of the wheatgrass group rats (3rd and 4th groups) once a day. This practice lasted for 4 weeks. Total oxidant, antioxidant quantities, DNA damage were evaluated at the end of 4 weeks. The result that wheatgrass could be useful in renal failure was reached.Öğe Buğday çiminin diyabetli ratlarda bazı hematolojik ve lipit parametrelerine etkisi(Young Scholars Union, 2017-08-22) Mis, Leyla; Comba, Bahat; Yeltekin Çilingir, Aslı; Uslu, SemaBu çalışmada deneysel diyabet oluşturulmuş ratlarda buğday çiminin kan parametrelerine ve lipit profili üzerine etkisinin araştırılması amaçlandı. Çalışmada kullanılan ratlar rastgele seçilerek her biri 8 rattan oluşan 4 gruba ayrıldı: Kontrol grubu, tek doz 1 ml fosfat-sitrat tamponu (pH: 4.5) verildi., Diyabet grubu;45 mg/kg tek doz streptozotosin ip enjeksiyon yapıldı., Buğday çimi grubu; ağızdan 10ml/kg/gün 6 hafta buğday çimi ektraktı verildi, Diyabet+Buğday çimi grubu;45 mg/kg tek doz streptozotosin ip enjeksiyon yapıldı ve ağızdan 10ml/kg/gün 6 hafta buğday çimi ektraktı verildi. Deneysel çalışma tamamlanınca 6 haftanın sonunda kan örnekleri alındı.Hematolojik parametreler kan sayım cihazı ile bakıldı. Elde edilen serumlarda trigliserit,kolesterol,LDL, VLDL, UHDL, düzeyleri otoanalizörde analiz edildi.RBC(p? 0.05),HCT(p? 0.05),PLT(p? 0.05),%LY(p? 0.05), değerleri diyabet grubunda düştüğü, Diyabet +Buğday çimi grubunda yükseldiği; WBC(p? 0.05),LYM(p? 0.05),MO(p? 0.05),NEU(p? 0.05),%MO(p? 0.05),%NEU(p? 0.01),düzeylerinin diyabet grubunda yükseldiği Diyabet +Buğday grubunda düştüğü istatistiki olarak belirlendi.Diyabet+buğdayçimi grubunda ise diyabet grubuna göre trigliserit(p? 0.05),VLDL(p? 0.05)düzeylerinin istatistiki olarak önemle azaldığı HDL(p? 0.05) düzeyinin yükseldiği tespit edildi.Serum kolesterol düzeylerinde gruplar arası önem bulunmadı.Sonuç olarak; buğday çiminin STZ ye bağlı diyabette oluşan hematolojik ve bazı serum lipit düzeyindeki bozuklukların düzeltilmesinde etkili olabileceği düşünüldü.Bu değişimlerin açıklanabilmesi için uzun süreli ve moleküler çalışmalar yapılmalıdır.Öğe Çorum ilinde sokak köpeklerini enfeste eden kene türlerinin belirlenmesi(Refik Saydam National Public Health Agency (RSNPHA), 2020) Arslan Akveran, Gönül; Karasartova, Djursun; Comba, Arzu; Comba, Bahat; Keskin, Adem; Taylan Özkan, Hikmet AyşegülObjective: The Province of Çorum is an endemic area for tick-borne diseases such as the Crimean Congo hemorrhagic fever. The aim of this study was to identify the tick species infesting stray dogs found in the surrounding of human habitations. Methods: Hundred stray dogs kept in the Animal Nursing Home of the Veterinary Service of the province were randomly selected during the period of April 2018 and March 2019. Ticks were removed with the help of forceps and placed in 96% ethyl alcohol and stored at +4°C until they were taxonomically identified. Results: The following tick species were found: Ixodes kaiseri, Hyalomma spp., Rhipicephalus sanguineus, Rhipicephalus turanicus and Haemaphysalis parva. The infestation prevalence was 20%, the infestation density 3.3 (1-7) and the abundance was 0.7. The highest numbers of tick were recorded in June and August, while the lowest in April and May. Conclusion: The number of ticks collected from dogs in this study was relatively low, showing that the efforts of the Veterinary Services to control ticks infesting dogs, is successful. This should lower the possibilities of tick-borne diseases and zoonoses which could be transmitted by stray dogs in the region. © 2020. Turk Hijyen ve Deneysel Biyoloji Dergisi. All rights reserved.Öğe Diyabet oluşturulmuş ratlarda arpa çiminin tüy mineral seviyelerine etkileri(Young Scholars Union, 2017-08-23) Yaşar, Semih; Comba, Bahat; Mis, Leyla; Comba, ArzuDiyabet ve oluşturduğu komplikasyonların önlenmesi günümüzde büyük önem oluşturmaktadır. Arpa çimi bu konuda ümit vadetmektedir. Bu çalışmada diyabet oluşturulmuş ratlarda arpa çiminin (AÇ) tüy mineral düzeylerine etkisinin araştırılması amaçlandı. Arpa, dünyanın en kapsamlı yeti Ştirilen ekinlerinden biridir. Arpa çimi (AÇ), arpanın yeni filizleridir ve çok çeşitli besin maddeleri ve bitki hormonları içerir. Diyabet, dünyada giderek büyüyen kronik bir metabolizma bozukluğudur. Bazı makro ve iz elementlerin memelilerde bir takım fizyolojik fonksiyonlar için gerekli olduğu kabul edilmektedir. Mineral fazlalığı veya yetersizliğine bağlı dengesizlikler bazı patolojik durumlarla ilişkili bulunmuştur. Bu çalışmada her grupta 6 rat bulunan dört grup olu Şturuldu; Kontrol (K) grubuna 7 gün için i.p. olarak fizyolojik tuzlu su enjekte edildi; Diyabet (DM) grubuna bir kez Streptozotosin (45 mg/kg) i.p. olarak enjekte edildi; Arpa çimi (AÇ) grubuna 4 hafta süresince oral olarak 3 ml/rat/gün arpa çimi suyu verildi; DM+AÇ grubuna bir kez Streptozotosin (45 mg/kg) i.p. olarak enjekte edildi ve 4 hafta boyunca oral olarak 3 ml/rat/gün arpa çimi suyu verildi. 4 hafta sonunda tüm ratlardan hafif sedasyon altında tüyler alındı. Diyabet grubunda Cu ve Zn elementlerinin düzeylerinde kontrol grubuna kıyasla azalmalar görüldü. Na elementi düzeylerinde DM+AÇ ve AÇ gruplarında kontrole kıyasla bir azalma, DM grubunda ise kontrole göre bir artış gözlendi. Diyabette arpa çimi kullanımı denenebilirÖğe Effect of Wheatgrass on DNA damage, oxidative stress ındex and histological findings in Diabetic Rats(Soc Chilena Anatomia, 2018-12) Mis, Leyla; Comba, Bahat; Uslu, Sema; Yeltekin, AslıThis study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer injected i.p (pH: 4.5), Diabetes group; 45 mg/kg single dose streptozotocin injected i.p., Wheatgrass group; was given oral wheatgrass (10 ml/kg/day) for 6 weeks, Diabetes +Wheatgrass group; 45 mg/kg single dose streptozotocin injected i.p. and wheatgrass (10 mlikg/day) was given by oral during 6 weeks. After the process of experiment during 6 weeks, blood sample and pancreas tissue were taken. The analysis were done of blood glucose levels, TAS, TOS levels by colorimetric kits; DNA damage by ELISA kits in serum. The pancreas tissues were examined histopathologically. In the group of Diabetes+Wheatgrass was determined that the levels of glucose levels (p<0.001),TOS (p<0.05) and OSI (p<0.01) statistically decreased and heal histopatolojical compared to diabetes group. In the group of Wheatgrass was determined that the levels of TAS p<0.05 statistically increased from other groups. The statistical significance were not found in the level of serum 8OHdG differences between the groups. The beta cells were seen to increase in the group receiving wheatgrass for therapeutic purposes.As a conclusion, it was determined that wheatgrass strengthened the anti-oxidant defense system and reduced the glucose level in diabetic rats.Öğe EFFECTS OF BORIC ACID ON PROINFLAMMATION CYTOKINES, TOTAL OXIDATIVE-ANTIOXIDATIVE STATUS AND HEMATOLOGICAL PARAMETERS IN RATS APPLIED BENZO(a)PYRENE(Parlar Scientific Publications (P S P), 2020) Comba, Arzu; Oto, Gokhan; Comba, Bahat; Ozdemir, Hulya; Keskin, Siddik; Akveran, Gonul ArslanThe aim of this study was to evaluate effects of boric acid (BA) on tumor necrosis factor-alfa (TNF-alpha), interleukin 1 beta (IL-beta), interleukin-6 (IL-6), total oxidative (TOS)-total anti oxidative status (TAS) and leucocyte (WBC), erythrocyte (RBC), thrombocyte (PLT) and their other parameters in rats applied benzo(a)pyrene (B(a)P). In this study, rats were divided into 4 groups and each group were containing 6 rats. 1st group was separated as control group. 100 mg/kg total dosage of B(a)P was applied to the 2nd group 2 times a week total in 4 equal dosage with i.p. way. Only BA 300 mg/L was applied to 3rd group in drinking water every day during 150 days. B(a)P was applied twice a week first 2 weeks, 25 mg/kg dose with i.p. way and BA were given with drinking water during 150 days to 4th group. In the end of study was determined TNF-alpha and IL-1 beta levels in B(a)P group, increases compared with other groups. there was a statistically significant increase in levels of WBC and LY in group B(a)P+BA compared to other groups (P <= 0.05). % LY level in other groups was higher; its % NEU level was lower according to control group. RBC and PLT values in BA group were higher, than the other groups. HGB was higher in group BA than group B(a)P and BA+ B(a)P; HCT was higher in group BA than group B(a)P , significantly (P <= 0.05) As a result, neutropenia and lenfositos develop in case exposure of B(a)P and BA; furthermore, combination of B(a)P and BA increases WBC. BA used alone having considerable effect on excitation of eritopoezis and thrombocytosis. BA has protective effects for organisms together with decrease in the TNF-alpha and IL-1 beta levels caused by BA cure as solution of B(a)P exposition.Öğe How long-term intake of sodium fluoride (NAF) in different doses and 7,12 dimethylbenz(A)anhtracene (DMBA) affect the erythrocyte parameters in rats?(Pas Forum, 2019) Comba, Bahat; Oto, Gökhan; Arıhan, Okan; Comba, Arzu; Uyar, HasanThis study was aimed to search the effect of Sodium Fluoride (NaF) and 7,12 dimethylbenz(a)anthracene (DMBA) to erythrocyte fragility and parameters in rats. The nine groups were formed and each group contained 8 animals. Group 1: Control group (without any treatment). Group 2: Sesame oil (vehicle for DMBA). Group 3: 1 ppm NaF. Group 4: 15 ppm NaF. Group 5: 30 ppm NaF. Group 6: DMBA.Group 7: 1 ppm NaF + DMBA. Group 8: 15 ppm NaF + DMBA. Group 9: 30 ppm NaF + DMBA. Fluoride was added into the animals’ drinking water in the form of NaF once a day, for 12 weeks. DMBA (10 mg/kg) was administered once a week and in a total of 12 weeks with oral gavage. Erythrocyte fragility was analyzed with osmotic hemolysis method and erythrocyte parameters with blood cell counter in whole blood. At 0.4% NaCl concentration groups 4, 5, 6, 8, and 9 showed significantly higher erythrocyte fragility values than control group (p?0.05). At 0.5% NaCl concentration, groups 6, 8 and 9 showed significant increase in erythrocyte fragility compared to other groups (p?0.05). The erythrocyte and hematocrit values were found significantly high in group 5 (p?0.001) and group 4 (p?0.01) while it was found low in all groups with DMBA (6, 7, 8, 9) (p?0.05) compared to control group. Hemoglobin amount in group 5(p?0.01) and group 4 (p?0.05) were significantly higher than other groups. MCV and MCH in group 5 were significantly lower and these values in all groups with DMBA (6, 7, 8, 9) were determined significantly high compared to other groups. RDWC in group 5 (p?0.001) and group 4 (p?0.01) and in all groups with DMBA (6, 7, 8, 9) (p?0.05) was significantly increased compared to other groups. As a result, exposure to high doses of floride and DMBA may cause augmented erythrocyte fragility, abnormal erythrocyte parameters and anemia. Therefore, measures must be taken to protect the health of all living organisms in area exposed to high levels of fluoride and DMBA.Öğe How Long-term Intake of Sodium Fluoride (NaF) in Different Doses and 7,12 dimetilbenz(a)antrasen (DMBA) Affect Erythrocyte Fragility and Parameters in Rats?(Wiley, 2017) Comba, Bahat; Oto, Gokhan; Arihan, Okan; Comba, Arzu; Uyar, Hasan[Abstract Not Available]Öğe INVESTIGATION OF THE IMMUNOCASTRATION EFFICACY AND LONGEVITY OF A SINGLE DOSE GNRH VACCINE IN YOUNG MALE RATS(Parlar Scientific Publications (P S P), 2019) Eski, Funda; Mis, Leyla; Tasal, Ibrahim; Uslu, Baris Atalay; Comba, BahatConventionally, immunization protocol require an initial priming immunization followed by an antigen boost for adequate immunity and long-term immunization. In this study we were evaluate the immunocastration efficacy and longevity of immunization with a single-dose GnRH vaccine (Repro-Bloc (TM)) in young male rats. Rats in the vaccine group (VAC, n: 35) were treated with GnRH vaccine as single-dose (200 mu l) and the control group (C, n: 15) were also injected with saline solution. Blood samples from all rats were taken monthly for 6 months and at 12th month. GnRH antibody titers, testosterone and leptin levels were determined by commercial RIA kits. Rats at 6th and 12th months were sacrificed for histologic analysis of testes. GnRH antibody levels were found similar in the VAC and C groups (P>0.05) at -1 week. As compared to the C, GnRH antibody levels (pg/ml) of the VAC group at 1st, 2nd, 3rd and 4th months rose continuously and reached the peak level at 5th month, decreased at 6th month and increased again by the 12th month (P<0.05), whereas in the C group GnRH antibodies weren't different (P>0.05). Compared to the unchanged testosterone levels within the process in C group, serum testosterone levels of the VAC group were lower at 2nd month and higher at 3th month, and thereafter lower (P<0.05). Leptin levels were no difference between groups (P>0.05). There were significant differences in the testicle weights, sperm motility, sperm numbers and abnormal sperm rates between VAC and C groups at 6th and 12th months (P< 0.05). Histological examination showed no significant difference in testes between groups at 6th and 12th months (P>0.05). In conclusion, although the vaccine reduced the germinative and endocrine testicular function from 6th months to 12th months with the single-dose GnRH vaccine, the single-dose vaccination did not provide adequate effect in the genital organs in male rats. Further studies are needed to determine an optimal single-dose, longevity of the vaccine and route of administration for complete suppression on the genital organs in different animal species.Öğe Karagül ve Norduz koyunlarında bazı hematolojik ve biyokimyasal parametreler(Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi, 2017-10-24) Comba, Bahat; Mert, Handan; Comba, Arzu; Mis, Leyla; Mert, NihatBu çalışma da aynı yaş, bakım ve besleme şartlarında sağlıklı Karagül ve Norduz koyun ırklarının bazı hematolojik ve biyokimyasal parametrelerinin karşılaştırılması amaçlandı. Her bir koyun ırkından 15 adet olmak üzere toplam 30 hayvan çalışmaya dahil edildi. Tam kan alyuvar sayısı (RBC), hemoglobin (HGB), hematokrit (HCT), ortalama alyuvar hacmi (MCV), ortalama alyuvar hemoglobini (MCH), ortalama alyuvar hemoglobin konsantrasyonu (MCHC), alyuvarların dağılım sıklığı (RDWC), akyuvar (WBC), lenfosit (LYM), monosit (MON), granülosit (GRN) sayıları, lenfosit (LY), monosit (MO), granülosit (GR) yüzdeleri, trombosit sayısı (PLT), trombosit yüzdesi (PCT), ortalama trombosit hacmi (MPV), trombosit dağılım sıklığı (PDWC) gibi hematolojik parametreleri kan sayım cihazında; serum alkalen fosfataz (ALP), aspartat aminotransferaz (AST), alkali fosfataz (ALP), gamma-glutamil transpeptidaz (GGT), total protein (TP) albümin (A), globulin (G) total bilirubin (TB) indirekt bilirubin (İB), kreatin, kreatin kinaz (CK), kreatin kinaz MB (CK-MB), üre, ürik asit (UA), kan üre azotu (BUN) ve amilaz biyokimyasal parametreleri oto analizörde belirlendi. HCT (p<0.001), MCV (p<0.001) ve MCH (p<0.001), CK (p<0.05) ve amilaz (p<0.001) Karagül koyun ırkında; LYM (p<0.05), RDWC (p<0.001), PLT (p<0.001), PCT (p<0.001), TP (p<0.05) ve IB (p<0.001) ise Norduz koyun ırkında daha yüksekti. Diğer parametrelerdeki değişimler önemsizdi. Sonuç olarak, Karagül ve Norduz koyun ırklarına ait bazı hematolojik ve biyokimyasal parametrelerdeki farklılıklar ırklara ait bir özellik olabilir. Elde edilen verilerin özellikle Veteriner Hekimlere, bu koyun ırklarına ait hastalıkların erken tanısında ve tedavisinde, prognozunda ve de bu ırklar ile ilgili yapılacak araştırmalarda referans olabileceği kanısındayız.Öğe Ratlarda farklı dozlarda Sodyum Florür (Naf)’ ün ve Dimetilbenz(A)Antrasen (DMBA)’ in uzun süreli alımı Eritrosit kırılganlığını ve parametrelerini nasıl etkiler?(Pamukkale Üniversitesi, 2017-09-10) Comba, Bahat; Oto, Gökhan; Arıhan, Okan; Comba, Arzu; Uyar, HasanGİRİŞ-AMAÇ: Bu çalışmada sıçanlarda Sodyum Florür (NaF) ve 7,12 dimetilbenz(a) antrasen (DMBA) ‘nin eritrosit kırılganlığına (EK) ve parametrelerine olan etkisinin araştırılması amaçlandı. YÖNTEM: Dokuz grup oluşturuldu ve her grupta 8 hayvan yer aldı. Grup 1: Kontrol. Grup 2: Susam yağı. Grup 3: NaF1 Grup 4: NaF15. Grup 5: NaF30. Grup 6: DMBA. Grup 7: NaF1 + DMBA. Grup 8: NaF15 + DMBA. Grup 9: NaF30 + DMBA. Hayvanların içme suyuna, günde bir kez NaF (ppm) ve haftada bir kez DMBA (10 mg/kg) 12 hafta gavaj ile uygulandı. EK, spektrofotometrik yöntemle çalışıldı. Veriler Anova ve Duncan testi ile değerlendirildi BULGULAR: % 0.4 NaCl konsantrasyonda EK, Grup 4, 5, 6, 8 ve 9’ da kontrol grubuna göre anlamlı olarak daha yüksekti Grup 5’deki MCV ve MCH, anlamlı olarak en düşüktü ve bu değer DMBA’lı tüm gruplarda (6, 7, 8, 9) diğer gruplara kıyasla belirgin olarak yüksek tespit edildi. RDWC’ de, Grup 5 (p SONUÇ: Sonuç olarak, yüksek dozdaki florür ve DMBA’ya maruz kalma, EK’na, anormal eritrositlere ve anemiye neden olabilir. Bu nedenle, yüksek seviyedeki florür ve DMBA’ya maruz kalan bölgede yaşayan tüm canlıların sağlığını korumak için acil tedbirler alınmalıdır.Öğe Retinol, ?-tocopherol and vitamin D3 in White Muscle disease(Medycyna Weterynaryjna, 2018-01-26) Mert, Handan; Yıldırım, Serkan; Yörük, İbrahim Hakkı; Irak, Kıvanç; Comba, Bahat; Mert, Nihat; Aysin, Nesrullah; Comba, ArzuVitamins are essential for the health of all living organisms. Vitamins E, A, D and K are known as fat-soluble vitamins, and deprivation of vitamin E causes various disorders, especially in the reproduction and cardiovascular systems and in muscle functions. Vitamin A, on the other hand, has roles in various biological functions – like eyesight – and the growth, reproduction and differentiation of epithelial cells. Vitamin A deficiency leads to the keratinization of the epithelium, and disorders related to the metaplasies of the genital and genitourinary systems. Conversely, vitamin D is defined as a pro-hormone and is responsible for Cahomeostasis, and thus indirectly affects the bone metabolism, bone structure, and cellular and neural functions of Ca. White muscle disease (WMD) can occur in newborn lambs, but is more commonly seen in lambs of up to 3 months of age. In this study, 30 lambs of 3 to 50-days-old from different flocks diagnosed with White Muscle Disease (WMD) were selected as research material, while the control group consisted of 8 healthy lambs. With the aim of clarifying the cause of WMD, serum fat-soluble vitamins, retinol, ?-tocopherol and vitamin D3 levels were determined in 16 lambs. Gluteal and heart musclet issue samples also were taken from 30 lambs with WMD. The vitamin levels of the samples were analysed by HPLC. The levels of serum ?-tocopherol, retinols, and vitamin D3 were foundto be low in the diseased animals, but only retinol (p<0.001) and ?-tocopherol (p<0.0011) level differences were statistically relevant. Macroscopically, Zenker’s necrosis was determined in the heart muscles of 17 lambs, and in the gluteal and chest muscles of 6 lambs. 7 lambs displayed necrosis in both their heart and in gluteal muscles. The samples were analyzed microscopically to reach similar findings: swollen homogeneous pink muscles, pycnotic nuclei, and hyperaemic and haemorrhagic blood vessels in gluteal, chest and heart muscles. Hyaline degeneration and Zenker's necrosis, dystrophic regions in necrotic areas, cc was detected as a severe disease in lambs at an early stage of life with advanced degeneration in different muscle tissues. Deficiency of fat-soluble vitamins was also detected in the sick animals. Control group lambs had higher levels of ? tocopherol and retinol (p<0.001) compared to the sick lambsÖğe Retinol, alpha-tocopherol and vitamin D-3 in White Muscle Disease(Polish Soc Veterinary Sciences Editorial Office, 2018) Mert, Handan; Yildirim, Serkan; Yoruk, İbrahim Hakkı; Irak, Kıvanç; Comba, Bahat; Mert, Nihat; Comba, ArzuVitamins are essential for the health of all living organisms. Vitamins E, A, D and K are known as fatsoluble vitamins, and deprivation of vitamin E causes various disorders, especially in the reproduction and cardiovascular systems and in muscle functions. Vitamin A, on the other hand, has roles in various biological functions - like eyesight - and the growth, reproduction and differentiation of epithelial cells. Vitamin A deficiency leads to the keratinization of the epithelium, and disorders related to the metaplasies of the genital and genitourinary systems. Conversely, vitamin D is defined as a pro-hormone and is responsible for Cahomeostasis, and thus indirectly affects the bone metabolism, bone structure, and cellular and neural functions of Ca. White Muscle Disease (WMD) can occur in newborn lambs, but is more commonly seen in lambs of up to 3 months of age. In this study, 30 lambs of 3 to 50-days-old from different flocks diagnosed with White Muscle Disease (WMD) were selected as research material, while the control group consisted of 8 healthy lambs. With the aim of clarifying the cause of WMD, serum fat-soluble vitamins, retinol, alpha-tocopherol and vitamin D3 levels were determined in 16 lambs. Gluteal and heart musclet issue samples also were taken from 30 lambs with WMD. The vitamin levels of the samples were analysed by HPLC. The levels of serum alpha-tocopherol, retinols, and vitamin D3 were foundto be low in the diseased animals, but only retinol (p < 0.001) and alpha-tocopherol (p < 0.001) level differences were statistically relevant. Macroscopically, Zenker's necrosis was determined in the heart muscles of 17 lambs, and in the gluteal and chest muscles of 6 lambs. 7 lambs displayed necrosis in both their heart and in gluteal muscles. The samples were analyzed microscopically to reach similar findings: swollen homogeneous pink muscles, pycnotic nuclei, and hyperaemic and haemorrhagic blood vessels in gluteal, chest and heart muscles. Hyaline degeneration and Zenker's necrosis, dystrophic regions in necrotic areas, cc was detected as a severe disease in lambs at an early stage of life with advanced degeneration in different muscle tissues. Deficiency of fat-soluble vitamins was also detected in the sick animals. Control group lambs had higher levels of alpha-tocopherol and retinol (p < 0.001) compared to the sick lambs.Öğe Some mineral substance, oxidative stress and total antioxidant levels in Norduz and Morkaraman sheep(Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi, 2018-10-09) Mis, Leyla; Mert, Handan; Comba, Arzu; Comba, Bahat; Doğan Söğütlü, İnci; Irak, Kıvanç; Mert, NihatThe aim of the study was to compare some mineral substances and total antioxidant and totaloxidan levels of healthy Morkaraman and Norduz sheep breeds in the same age and care conditions. A total of 20 animals, 10 from each sheep breed, were included in the study. Serum total oxidant status (TOS) and total antioxidant status (TAS) were determined by a colorimetric method. OSI value was determined by calculating the TASTOS ratio. When the two sheep were compared, there was a difference between TAS, Mg, P levels. Antioxidant levels of Norduz sheep were higher than morkaraman sheep. The given data may be the reference value for studies of these sheep breedsÖğe THE EFFECT OF BARLEY GRASS ON ANTIOXIDANT CAPACITY AND DNA DAMAGE IN RAT WITH RENAL FAILURE(Parlar Scientific Publications (P S P), 2017) Comba, Bahat; Mis, Leyla; Uslu, Sema; Comba, ArzuThis study was aimed to search the effect of barley grass (BG) on the Total Antioxidant (TAS)-Oxidant Status (TOS) and DNA damage (8OHdG) in rat with renal failure. The rats which are used in the study were randomly divided into 4 groups that each of has 8 rats: Control (C) group; injected i.p. with physiological saline once a day for 7 days, GM group; Gentamicin (80 mg/kg/day) was injected i.p. for 7 days, BG group; was given oral BG (250 mg/kg/day) for 4 weeks, GM+BG group was injected gentamicin (80 mg/kg/day) with i.p. for 7 days and BG (250 mg/kg/day) was given by oral for 4 weeks. After from the process of experiment for 4 weeks, blood sample and kidney tissue were taken. The analyses of urea and creatinine were done by autoanalyser; TAS, TOS levels by colorimetric kits; 8OHdG level by ELISA kits in serum. The kidney tissues were examined histologically. In the GM+BG group was statistically decreased levels of urea, creatinine, TOS (p<0.05) and OSI (p<0.01) compared to GM group. In the group of BG was determined the levels of TAS p<0.05 statistically increased other groups. The statistical significance was not found in the level of serum 8OHdG differences between the groups. BG decreased widespread damage to the renal glomerulus and proximal tubulus. As a result, adverse effects of high dose gentamicin application such as kidney damage can be reduced when used barley grass.Öğe The effect of chemical and surgical castration on the live weight gain and some hormones of male Norduz sheep(Zoological Socety of Pakistan, 2017-10) Karakuş, Kadir; Comba, Bahat; Taş, Abuzer; Sancak, Tunahan; Comba, Arzu; Sarıpınar Aksu, Devrim; Koyun, Hasan; Tariq, Mohammad MasoodThe aim of this study was to investigate the effects of surgical and chemical castration on body weight and some hormones. In the present study, 2.5-3-month-old weaned male Norduz sheep were used. The sheep were obtained in the year 2015 from Livestock Application and Research Directorate of Yuzuncu Yil University. The sheep were randomly allocated into three groups each of which contained 10 sheep. The groups were formed as; Group 1 The Control Group (No castration), Group 2 (Surgical castration group) and Group 3 (Chemical castration group), respectively. Blood sample was taken from vena jugularis of the animals before the application and every two months for a total of 4 times for 6 months and hormone analyses were performed. The order of statistical significance for TSH (thyroid stimulating hormone) were found as Surgical > Control > Formol (P<0.05). The order of statistical significance for growth hormone was Formol = Surgery > Control (P<0.05). In August month, the application factor was found to be significant only in testosterone (P<0.05). No significant difference between the control and surgical groups in testosterone was noted. In this study, although the application of different castration methods in 4-month-old male Norduz sheep resulted in changes in the blood hormone levels, it was determined that these changes did not affect body weight. In conclusion, the evaluation of the process in castrated six-month-old sheep revealed an increase in the body weight which was similar to that in the control group.Öğe The Effect of Cinnamaldehyde on The Activity of Glucose-6-Phosphate Dehydrogenase, Some Biochemical And Hematological Parameters in Diabetic Rats(Wiley, 2017) Celik, Remzi; Mert, Handan; Comba, Bahat; Mert, Nihat[Abstract Not Available]
