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    Öğe
    Possible Association of PER2/PER3 Variable Number Tandem Repeat Polymorphism Variants with Susceptibility and Clinical Characteristics in Pancreatic Cancer
    (Mary Ann Liebert, Inc, 2021) Dagmura, Hasan; Yigit, Serbulent; Nursal, Ayse Feyda; Duman, Esra; Gumusay, Ozge
    Objective: Pancreatic cancer (PC) is a serious disease with poor outcomes, and its prevalence has been increasing steadily. The circadian rhythm (CR) is involved in multiple physiological events and maintains homeostasis. Alterations in the CR elevate the risk of developing cancer. The present case-control research was carried out to estimate the possible association between PERIOD2/PERIOD3 (PER2/PER3) gene variable number tandem repeat polymorphism (VNTR) variants and PC in the Turkish population. Materials and Methods: A total of 198 subjects (78 patients with PC and 120 healthy controls) were enrolled in this work. Genomic DNA was collected from peripheral blood mononuclear cells, and genotype analysis was performed using a polymerase chain reaction (PCR) method. Odds ratio (OR) with a 95% confidence interval (95% CI) was calculated using the chi(2) test. Results: The frequency of the 4R (4 repeats)/3R (3 repeats), 3R/3R genotypes, and 3R allele of PER2 VNTR in patients with PC was significantly higher than in the control group (p = 0003, p = 0.00004, respectively). PER2 VNTR 4/5 genotype was related to perineural invasion (p = 0.040). The genotype and allele distribution of PER3 VNTR variant did not show any statistical difference between the two groups (p > 0.05). PER2/PER3 VNTR 4/5-4R/3R combined genotype increased in the patient group (p = 0.013), while 4/5-4R/4R combined genotype was superior in the control group (p = 0.0001). Conclusions: Our work has indicated that PER2 VNTR 3R allele may play a crucial task in the pathogenesis of PC in Turkish patients, which may become a useful marker for predicting the development of PC. Furthermore, the PER2 VNTR genotype seems to be related to perineural invasion in PC.
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    Öğe
    The Role of Interleukin-4 VNTR Polymorphism in Dysmenorrhea Development
    (2020) Esen, Mehmet; Nursal, Ayşe Feyda; Duman, Esra; Yiğit, Serbülent
    Aim: Primary dysmenorrhea (PD) is among the most common gynecological diseases in young women presenting to emergency department. It has been shown that cytokines played roles in PD pathogenesis. Interleukin-4 (IL-4), a cytokine, regulates multiple biological functions. The objective of the present study was to examine possible relationship between IL-4 variable number of tandem repeat (VNTR) polymorphism and susceptibility to PD. Methods: This study was based on a prospective cohort study design. A total of 120 patients with PD and 116 healthy controls, who presented to the emergency department between 01.12.2018 and 01.12.2019, were included in the study. IL-4 VNTR was genotyped by polymerase chain reaction (PCR). The results of the analyses were evaluated in terms of statistically significant differences. Results: The prevalence of genotypes of P1/P1, P1/P2, and P2/ P2 for IL-4 VNTR were 1.72%, 34.4%, and 63.7% in patients with PD, and 0.8%, 26.6%, and 72.5% in controls, respectively. There was no significant difference in distribution of genotypes and allele frequencies of IL-4 VNTR between the groups (p>0.05). Conclusion: This research is the first study to examine the relationship between IL-4 VNTR and PD. The data of the present study did not support a relationship between IL-4 VNTR and PD risk.