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    Effects of nickel sulphate and lead acetate trihydrate on heavy metal stress-related gene activities in forage pea (Pisum sativum ssp. arvense L.) in Türkiye
    (FRONTIERS MEDIA SA, 2025) Mesci, S; Çatal, MI
    Researching heavy metal stress in plants is of paramount importance due to the increasing prevalence of heavy metal contamination in the environment, which poses significant risks to both plant, animal, and human health. Limited data are available on heavy metal stress-related gene responses to pollutants such as nickel sulphate and lead acetate in forage peas (Pisum sativum ssp. arvense). This study aimed to investigate how specific stress-related genes respond to stress factors such as nickel sulphate and lead acetate in this plant species. In our study, we treated three cultivars of Pisum sativum ssp. arvense with nickel sulfate (20 and 40 mg/L) and lead acetate trihydrate (20 and 40 mg/L). We then measured the expression of heavy metal stress-related genes (APX, CAT, MT, PCS) using qRT-PCR on three pea cultivars (Kurtbey, Kirazl & imath;, and Pembe) in Rize, T & uuml;rkiye. Down-regulations in high heavy metal treatments and heavy metal gene-associated stress tolerance expressions were detected. Additionally, high up-regulations in APX, CAT, MT and PCS gene expressions were detected mostly at high nickel sulphate and lead acetate trihydrate applied rates. The study presents up-to-date contributions to biochemical and molecular data on the effects of nickel sulfate and lead acetate trihydrate toxicity on pea plants. These insights may inform strategies to breed or produce more heavy metal resistant crop varieties.
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    Öğe
    In Silico Molecular Docking of 2-Hydroxyanthraquinone-Substituted Spiro-/Ansa Cyclotriphosphazenes: Targeting Apoptosis via Heat Shock Protein Modulation in Breast and Colon Cancer Cells
    (WILEY, 2025) Mesci, S; Yazgan, B; Demirel, GD; Yıldırım, T; Çiftçi, GY
    Cancer research takes a long time and is complex. Preclinical studies prove that compounds can be potential anticancer agents and contribute to cancer studies. Overexpression of survivin may cause decreased sensitivity of anticancer agents and antiapoptotic activation through its excretion from cells via MDR. Anthraquinones and phosphazene compounds, which are among the active biological compounds and identified in many studies on cancer, come to the fore in biochemical, microbiological, and pharmacological studies. In this study, it was aimed to investigate the effect of 2-hydroxyanthraquinone-substituted spiro-/ansa cyclotriphosphazene compounds (II-VIII) on multidrug resistance, ER stress, and apoptotic cell death pathways in breast and colon cancer cells. mRNA expressions of multidrug resistance transporter, ER stress, heat shock, and apoptotic genes assessed by qPCR. Besides protein levels of apoptosis, cell cycle and related signaling pathways (CASP3, BCL-w, sTNF-R, cIAP-2, TRAILRs, IGFBPs, Survivin, XIAP, etc.) were determined by antibody membrane array method in MCF-7 and DLD-1 cell lines. To elucidate the activities of Survivin protein-related compounds, in silico-mediated molecular docking studies were evaluated. ABCs, HSPs, and GRPs gene expressions in MCF-7 and DLD-1 cells were decreased by these compounds. Besides, in gene regulations of apoptosis and signaling pathways, it was observed that the compounds induce overexpression of BAX and underexpression BCL-2. In addition, especially survivin expression was downregulated by all the compounds. It has been determined that the compounds eliminate multidrug resistance in breast and colon cancer cells, suppress HSPs and GRPs genes, and lead the cells to death, especially through the antiapoptotic pathway Survivin. These compounds can be evaluated and developed as Survivin inhibitor agents in anticancer studies.