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    ASSESSMENT OF THE WHEATGRASS EFFECTS ON ANTIOXIDANT CAPACITY AND DNA DAMAGE IN RATS WITH EXPERIMENTAL RENAL FAILURE
    (Parlar Scientific Publications (P S P), 2019) Mis, Leyla; Comba, Bahat; Basbugan, Yildiray; Yasar, Semih; Comba, Arzu
    In this study, it was aimed to evaluate the effect of wheatgrass on antioxidant capacity and DNA damage in rats with renal failure. In the study, 200-300 g 24 healthy Wistar-Albino male rats obtained from YYU Experimental Animal Unit were used. The rats were randomly divided into 4 groups and each group was formed with 6 rats. Groups were allocated as; 1. Control Group, 2. Renal Failure Group (Genta Group) 3. Renal Failure + Wheatgrass Group (Genta +Wheatgrass) 4. Wheatgrass Group. Gentamicin sulfate was given intraperitoneal at a dose of 50 mg/kg for 1 week and once a day to the groups with renal failure (Groups 2 and 3). Wheat grass extract (10 ml/kg) was added to the drinking water of the wheatgrass group rats (3rd and 4th groups) once a day. This practice lasted for 4 weeks. Total oxidant, antioxidant quantities, DNA damage were evaluated at the end of 4 weeks. The result that wheatgrass could be useful in renal failure was reached.
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    Buğday çiminin diyabetli ratlarda bazı hematolojik ve lipit parametrelerine etkisi
    (Young Scholars Union, 2017-08-22) Mis, Leyla; Comba, Bahat; Yeltekin Çilingir, Aslı; Uslu, Sema
    Bu çalışmada deneysel diyabet oluşturulmuş ratlarda buğday çiminin kan parametrelerine ve lipit profili üzerine etkisinin araştırılması amaçlandı. Çalışmada kullanılan ratlar rastgele seçilerek her biri 8 rattan oluşan 4 gruba ayrıldı: Kontrol grubu, tek doz 1 ml fosfat-sitrat tamponu (pH: 4.5) verildi., Diyabet grubu;45 mg/kg tek doz streptozotosin ip enjeksiyon yapıldı., Buğday çimi grubu; ağızdan 10ml/kg/gün 6 hafta buğday çimi ektraktı verildi, Diyabet+Buğday çimi grubu;45 mg/kg tek doz streptozotosin ip enjeksiyon yapıldı ve ağızdan 10ml/kg/gün 6 hafta buğday çimi ektraktı verildi. Deneysel çalışma tamamlanınca 6 haftanın sonunda kan örnekleri alındı.Hematolojik parametreler kan sayım cihazı ile bakıldı. Elde edilen serumlarda trigliserit,kolesterol,LDL, VLDL, UHDL, düzeyleri otoanalizörde analiz edildi.RBC(p? 0.05),HCT(p? 0.05),PLT(p? 0.05),%LY(p? 0.05), değerleri diyabet grubunda düştüğü, Diyabet +Buğday çimi grubunda yükseldiği; WBC(p? 0.05),LYM(p? 0.05),MO(p? 0.05),NEU(p? 0.05),%MO(p? 0.05),%NEU(p? 0.01),düzeylerinin diyabet grubunda yükseldiği Diyabet +Buğday grubunda düştüğü istatistiki olarak belirlendi.Diyabet+buğdayçimi grubunda ise diyabet grubuna göre trigliserit(p? 0.05),VLDL(p? 0.05)düzeylerinin istatistiki olarak önemle azaldığı HDL(p? 0.05) düzeyinin yükseldiği tespit edildi.Serum kolesterol düzeylerinde gruplar arası önem bulunmadı.Sonuç olarak; buğday çiminin STZ ye bağlı diyabette oluşan hematolojik ve bazı serum lipit düzeyindeki bozuklukların düzeltilmesinde etkili olabileceği düşünüldü.Bu değişimlerin açıklanabilmesi için uzun süreli ve moleküler çalışmalar yapılmalıdır.
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    Öğe
    Diyabet oluşturulmuş ratlarda arpa çiminin tüy mineral seviyelerine etkileri
    (Young Scholars Union, 2017-08-23) Yaşar, Semih; Comba, Bahat; Mis, Leyla; Comba, Arzu
    Diyabet ve oluşturduğu komplikasyonların önlenmesi günümüzde büyük önem oluşturmaktadır. Arpa çimi bu konuda ümit vadetmektedir. Bu çalışmada diyabet oluşturulmuş ratlarda arpa çiminin (AÇ) tüy mineral düzeylerine etkisinin araştırılması amaçlandı. Arpa, dünyanın en kapsamlı yeti Ştirilen ekinlerinden biridir. Arpa çimi (AÇ), arpanın yeni filizleridir ve çok çeşitli besin maddeleri ve bitki hormonları içerir. Diyabet, dünyada giderek büyüyen kronik bir metabolizma bozukluğudur. Bazı makro ve iz elementlerin memelilerde bir takım fizyolojik fonksiyonlar için gerekli olduğu kabul edilmektedir. Mineral fazlalığı veya yetersizliğine bağlı dengesizlikler bazı patolojik durumlarla ilişkili bulunmuştur. Bu çalışmada her grupta 6 rat bulunan dört grup olu Şturuldu; Kontrol (K) grubuna 7 gün için i.p. olarak fizyolojik tuzlu su enjekte edildi; Diyabet (DM) grubuna bir kez Streptozotosin (45 mg/kg) i.p. olarak enjekte edildi; Arpa çimi (AÇ) grubuna 4 hafta süresince oral olarak 3 ml/rat/gün arpa çimi suyu verildi; DM+AÇ grubuna bir kez Streptozotosin (45 mg/kg) i.p. olarak enjekte edildi ve 4 hafta boyunca oral olarak 3 ml/rat/gün arpa çimi suyu verildi. 4 hafta sonunda tüm ratlardan hafif sedasyon altında tüyler alındı. Diyabet grubunda Cu ve Zn elementlerinin düzeylerinde kontrol grubuna kıyasla azalmalar görüldü. Na elementi düzeylerinde DM+AÇ ve AÇ gruplarında kontrole kıyasla bir azalma, DM grubunda ise kontrole göre bir artış gözlendi. Diyabette arpa çimi kullanımı denenebilir
  • Küçük Resim
    Öğe
    Effect of Wheatgrass on DNA damage, oxidative stress ındex and histological findings in Diabetic Rats
    (Soc Chilena Anatomia, 2018-12) Mis, Leyla; Comba, Bahat; Uslu, Sema; Yeltekin, Aslı
    This study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer injected i.p (pH: 4.5), Diabetes group; 45 mg/kg single dose streptozotocin injected i.p., Wheatgrass group; was given oral wheatgrass (10 ml/kg/day) for 6 weeks, Diabetes +Wheatgrass group; 45 mg/kg single dose streptozotocin injected i.p. and wheatgrass (10 mlikg/day) was given by oral during 6 weeks. After the process of experiment during 6 weeks, blood sample and pancreas tissue were taken. The analysis were done of blood glucose levels, TAS, TOS levels by colorimetric kits; DNA damage by ELISA kits in serum. The pancreas tissues were examined histopathologically. In the group of Diabetes+Wheatgrass was determined that the levels of glucose levels (p<0.001),TOS (p<0.05) and OSI (p<0.01) statistically decreased and heal histopatolojical compared to diabetes group. In the group of Wheatgrass was determined that the levels of TAS p<0.05 statistically increased from other groups. The statistical significance were not found in the level of serum 8OHdG differences between the groups. The beta cells were seen to increase in the group receiving wheatgrass for therapeutic purposes.As a conclusion, it was determined that wheatgrass strengthened the anti-oxidant defense system and reduced the glucose level in diabetic rats.
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    INVESTIGATION OF THE IMMUNOCASTRATION EFFICACY AND LONGEVITY OF A SINGLE DOSE GNRH VACCINE IN YOUNG MALE RATS
    (Parlar Scientific Publications (P S P), 2019) Eski, Funda; Mis, Leyla; Tasal, Ibrahim; Uslu, Baris Atalay; Comba, Bahat
    Conventionally, immunization protocol require an initial priming immunization followed by an antigen boost for adequate immunity and long-term immunization. In this study we were evaluate the immunocastration efficacy and longevity of immunization with a single-dose GnRH vaccine (Repro-Bloc (TM)) in young male rats. Rats in the vaccine group (VAC, n: 35) were treated with GnRH vaccine as single-dose (200 mu l) and the control group (C, n: 15) were also injected with saline solution. Blood samples from all rats were taken monthly for 6 months and at 12th month. GnRH antibody titers, testosterone and leptin levels were determined by commercial RIA kits. Rats at 6th and 12th months were sacrificed for histologic analysis of testes. GnRH antibody levels were found similar in the VAC and C groups (P>0.05) at -1 week. As compared to the C, GnRH antibody levels (pg/ml) of the VAC group at 1st, 2nd, 3rd and 4th months rose continuously and reached the peak level at 5th month, decreased at 6th month and increased again by the 12th month (P<0.05), whereas in the C group GnRH antibodies weren't different (P>0.05). Compared to the unchanged testosterone levels within the process in C group, serum testosterone levels of the VAC group were lower at 2nd month and higher at 3th month, and thereafter lower (P<0.05). Leptin levels were no difference between groups (P>0.05). There were significant differences in the testicle weights, sperm motility, sperm numbers and abnormal sperm rates between VAC and C groups at 6th and 12th months (P< 0.05). Histological examination showed no significant difference in testes between groups at 6th and 12th months (P>0.05). In conclusion, although the vaccine reduced the germinative and endocrine testicular function from 6th months to 12th months with the single-dose GnRH vaccine, the single-dose vaccination did not provide adequate effect in the genital organs in male rats. Further studies are needed to determine an optimal single-dose, longevity of the vaccine and route of administration for complete suppression on the genital organs in different animal species.
  • Küçük Resim
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    Karagül ve Norduz koyunlarında bazı hematolojik ve biyokimyasal parametreler
    (Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi, 2017-10-24) Comba, Bahat; Mert, Handan; Comba, Arzu; Mis, Leyla; Mert, Nihat
    Bu çalışma da aynı yaş, bakım ve besleme şartlarında sağlıklı Karagül ve Norduz koyun ırklarının bazı hematolojik ve biyokimyasal parametrelerinin karşılaştırılması amaçlandı. Her bir koyun ırkından 15 adet olmak üzere toplam 30 hayvan çalışmaya dahil edildi. Tam kan alyuvar sayısı (RBC), hemoglobin (HGB), hematokrit (HCT), ortalama alyuvar hacmi (MCV), ortalama alyuvar hemoglobini (MCH), ortalama alyuvar hemoglobin konsantrasyonu (MCHC), alyuvarların dağılım sıklığı (RDWC), akyuvar (WBC), lenfosit (LYM), monosit (MON), granülosit (GRN) sayıları, lenfosit (LY), monosit (MO), granülosit (GR) yüzdeleri, trombosit sayısı (PLT), trombosit yüzdesi (PCT), ortalama trombosit hacmi (MPV), trombosit dağılım sıklığı (PDWC) gibi hematolojik parametreleri kan sayım cihazında; serum alkalen fosfataz (ALP), aspartat aminotransferaz (AST), alkali fosfataz (ALP), gamma-glutamil transpeptidaz (GGT), total protein (TP) albümin (A), globulin (G) total bilirubin (TB) indirekt bilirubin (İB), kreatin, kreatin kinaz (CK), kreatin kinaz MB (CK-MB), üre, ürik asit (UA), kan üre azotu (BUN) ve amilaz biyokimyasal parametreleri oto analizörde belirlendi. HCT (p<0.001), MCV (p<0.001) ve MCH (p<0.001), CK (p<0.05) ve amilaz (p<0.001) Karagül koyun ırkında; LYM (p<0.05), RDWC (p<0.001), PLT (p<0.001), PCT (p<0.001), TP (p<0.05) ve IB (p<0.001) ise Norduz koyun ırkında daha yüksekti. Diğer parametrelerdeki değişimler önemsizdi. Sonuç olarak, Karagül ve Norduz koyun ırklarına ait bazı hematolojik ve biyokimyasal parametrelerdeki farklılıklar ırklara ait bir özellik olabilir. Elde edilen verilerin özellikle Veteriner Hekimlere, bu koyun ırklarına ait hastalıkların erken tanısında ve tedavisinde, prognozunda ve de bu ırklar ile ilgili yapılacak araştırmalarda referans olabileceği kanısındayız.
  • Küçük Resim
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    Some mineral substance, oxidative stress and total antioxidant levels in Norduz and Morkaraman sheep
    (Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi, 2018-10-09) Mis, Leyla; Mert, Handan; Comba, Arzu; Comba, Bahat; Doğan Söğütlü, İnci; Irak, Kıvanç; Mert, Nihat
    The aim of the study was to compare some mineral substances and total antioxidant and totaloxidan levels of healthy Morkaraman and Norduz sheep breeds in the same age and care conditions. A total of 20 animals, 10 from each sheep breed, were included in the study. Serum total oxidant status (TOS) and total antioxidant status (TAS) were determined by a colorimetric method. OSI value was determined by calculating the TASTOS ratio. When the two sheep were compared, there was a difference between TAS, Mg, P levels. Antioxidant levels of Norduz sheep were higher than morkaraman sheep. The given data may be the reference value for studies of these sheep breeds
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    THE EFFECT OF BARLEY GRASS ON ANTIOXIDANT CAPACITY AND DNA DAMAGE IN RAT WITH RENAL FAILURE
    (Parlar Scientific Publications (P S P), 2017) Comba, Bahat; Mis, Leyla; Uslu, Sema; Comba, Arzu
    This study was aimed to search the effect of barley grass (BG) on the Total Antioxidant (TAS)-Oxidant Status (TOS) and DNA damage (8OHdG) in rat with renal failure. The rats which are used in the study were randomly divided into 4 groups that each of has 8 rats: Control (C) group; injected i.p. with physiological saline once a day for 7 days, GM group; Gentamicin (80 mg/kg/day) was injected i.p. for 7 days, BG group; was given oral BG (250 mg/kg/day) for 4 weeks, GM+BG group was injected gentamicin (80 mg/kg/day) with i.p. for 7 days and BG (250 mg/kg/day) was given by oral for 4 weeks. After from the process of experiment for 4 weeks, blood sample and kidney tissue were taken. The analyses of urea and creatinine were done by autoanalyser; TAS, TOS levels by colorimetric kits; 8OHdG level by ELISA kits in serum. The kidney tissues were examined histologically. In the GM+BG group was statistically decreased levels of urea, creatinine, TOS (p<0.05) and OSI (p<0.01) compared to GM group. In the group of BG was determined the levels of TAS p<0.05 statistically increased other groups. The statistical significance was not found in the level of serum 8OHdG differences between the groups. BG decreased widespread damage to the renal glomerulus and proximal tubulus. As a result, adverse effects of high dose gentamicin application such as kidney damage can be reduced when used barley grass.