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    Streptomyces coryli sp. nov., isolated from hazelnut orchard soil
    (Microbiology Soc, 2020) Saygın, Hayrettin; Veyisoğlu, Aysel; Tatar, Demet; Nigiz, Cengiz; Tokatlı, Ali; Şahin, Nevzat
    A novel actinobacteria, isolate A7024(T), was isolated from commercial hazelnut orchard soil sample which was collected at Duzce, West Black Sea region, Turkey. A polyphasic taxonomic study was carried out to determine the status of this isolate. The phylogenetic tree reconstructed using the neighbour-joining algorithm based on 165 rRNA gene sequences indicated that isolate A7024(T) was positioned within the members of the genus Streptomyces with the highest sequence similarity (97.7%) to Streptomyces cadmiisoli ZFG47(T). The organism formed an extensively branched substrate and aerial hyphae which generated irregular rod-shaped spores with smooth-surfaces. The cell wall of strain A7024(T) contained LL-diaminopimelic. Glucose, mannose and ribose were detected as whole-cell sugars. Its polar lipids were diphosphatidylglycerol. phosphatidylethanol-amine, phosphatidylinositol, three unidentified phospholipids and three unidentified glycolipids. Major menaquinones were MK-9(H-6) and MK-9(H-4). The major cellular fatty acids were iso-C-16:0, anteiso-C-1(5:0) and anteiso-C-1(7:)0. Strain A7024(T) had a genome size of 9.0 Mb with a genome G+C content of 71.5 mol%. The low level of 16S rRNA gene similarity, 19.3 +/- 2.3% digital DNA-DNA-hybridization and 76.94% average nucleotide identity values, as well as some different phenotypic characteristics allowed the strain to be distinguished from the closely related type strains. Therefore, it is concluded that strain A7024(T) represents a novel species of the genus of Streptomyces, for which the name Streptomyces coryli sp. nov. is proposed. The type strain is A7024(T) (=DSM 42066(T)=KCTC 29102(T)=NRRL B-24888(T)).
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    Synthesis, characterization and antibacterial application of silver nanoparticle embedded composite cryogels
    (Elsevier, 2020) Erol, Kadir; Bolat Bülter, Melda; Tatar, Demet; Nigiz, Cengiz; Köse, Dursun Ali
    Water treatment applications; they consist of processes that desalination, softening, removals of dissolved organic matter, color, particle and microorganism. In traditional treatment processes, microorganisms cause thick film layer on the membrane to be used and not enough treatment. Silver nanoparticles (AgNPs), known to stop bacterial growth due to their superior antibacterial properties even at low concentrations due to their contact with microorganisms, are used for water disinfection. Membrane production with a combination of AgNPs and polymers has started to attract attention in recent years due to advanced optics, conductivity and antibacterial properties. The ability of AgNP to exhibit its antibacterial effect depends on the property of this material to be homogeneously dispersed in the polymer. In this study, Poly(2-hydroxyethyl methacrylate-glycidyl methacrylate), Poly(HEMA-GMA), cryogels were synthesized as solid support. In order to gain antibacterial properties to synthesized cryogels, AgNPs of different sizes were embedded in cryogels. These structures have been used as a kind of antibacterial filter in the purification of water obtained from natural sources used as drinking and using water in the local region. Particle Size analysis was used to determine the size of synthesized AgNPs. Swelling test, Scanning Electron Microscopy (SEM), Elemental Analysis, Fourier Transform Infrared Spectroscopy (FT-IR), and surface area (BET) analysis were performed for characterization of cryogels. (C) 2019 Elsevier B.V. All rights reserved.