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    Is there any possibility that selenium and homocysteine play a role in metastatic ability of colorectal tumors?
    (Dustri-Verlag Dr Karl Feistle, 2020) Horozoglu, Cem; Rajab, Basem Mabruk M.; Turan, Saime; Erbasoglu, Oncu Koc; Kucukhuseyin, Ozlem; Hakan, Mehmet Tolgahan; Yaylim, Ilhan
    Introduction: The data suggesting that metabolites and genes of homocysteine may play a role in the pathogenesis of colorectal cancer are available in the literature. Similarly, selenium (Se) levels and SELENBP1, a member of the selenoprotein family characterized by selenium, have been evaluated for the etiopathology of colorectal cancer (CRC). In this study, we aimed to determine the levels of homocysteine (Hcy), Se, and SELENBP1 gene expression in tumor tissue according to histopathological stages in patients with CRC. Materials and methods: Hcy levels were determined by ELISA, and Se levels were determined by atomic absorption from serum samples isolated from peripheral blood of 56 colorectal cancer cases and 87 healthy controls. The expression of SELENBP1 gene in tumor samples was determined by RT-PCR method. The histopathological evaluations of the patients were evaluated according to the AJCC-8th staging system. Results: Se levels were 67.56 +/- 3.11 ng/mL in colorectal cancer patients and 61.92 +/- 21.26 ng/mL in control group patients (p = 0.078). Serum Hcy levels were 11.34 +/- 1.75 ng/mL in the patient group and 20.87 +/- 4.38 in the control group (p = 0.340). The SELENBP1 fold change was found to be 3.78 +/- 1.95-fold higher in tumor tissues compared to internal control (p = 0.755). Se levels were found to be 1.23 times lower in patients with distant metastasis than in patients without distant metastasis (p = 0.029). Similarly, Hcy level was found to be 1.79 times lower in patients with metastasis than in non-metastatic patients (p = 0.035). Conclusion: Our data suggest that low Se and Hcy levels may play an important role in the histopathological aspects of metastatic ability and CRC.
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    The importance of programmed death ligand 1 gene expression, epidermal growth factor receptor gene mutations and serum epidermal growth factor receptor levels in Turkish non-small cell lung cancer patients
    (Bayçınar Medical Publishing, 2018) Turna, Akif; Horozoğlu, Cem; Koç Erbaşoğlu, Öncü; Ercan, Şeyda; Küçükhüseyin, Özlem; Turan, Saime; Hakan, Mehmet Tolgahan; Kara, Hasan Volkan; Hekimoğlu, Elvin; Zeybek, Ümit; Coşkunpınar, Ender; Cacına, Canan; Ergen, Arzu; Yaylım, İlhan
    Background: This study aims to investigate the possible relationships between epidermal growth factor receptor gene mutations, serum epidermal growth factor receptor levels, programmed death ligand gene expression levels and the risks and survivals of resectable nonsmall cell lung cancer patients. Methods: Deoxyribonucleic acid isolation was performed from peripheral blood samples and tumor tissues. The mutation analysis was performed for epidermal growth factor receptor. Programmed death ligand 1 gene expression levels were examined pathologically and histopathologically following the tissue tracing of 36 non-small cell lung cancer patients (29 males, 7 females; mean age 60.1 years; range, 41 to 79 years) and analyzed using real-time polymerase chain reaction. Epidermal growth factor receptor serum levels were assessed in all patients. Results: As a result of mutation analyses in 21 patients (28.5% of all adenocarcinoma patients), epidermal growth factor receptor mutation was determined in at least one exon in six patients. In epidermal growth factor receptor mutation detected patients, programmed death ligand 1 gene expression levels were associated with lymph node metastasis (p=0.036). However, epidermal growth factor receptor mutations were not statistically significantly associated according to histopathological examination (p>0.05). Of patients carrying exon 20 (c.2303G>T) mutations, 25% had tumors with perineural invasion. There was a statistically significant association between exon 20 insertions and c.2303G>T and lymphatic invasion (p=0.02), lymph node metastasis and exon 20 insertions (p=0.03). Patients with lower serum epidermal growth factor receptor levels (<400 pg/mL) had better survival time than those with higher serum epidermal growth factor receptor levels (p=0.04). Conclusion: Programmed death ligand 1 gene expression and epidermal growth factor receptor mutation might have a combined effect on non-small cell lung cancer. Programmed death ligand 1 gene expression in tumor pathology may also be a significant feature for tumor progression and tumorigenesis. Serum epidermal growth factor receptor levels seem to be associated with survival. ©2018 All right reserved by the Turkish Society of Cardiovascular Surgery.