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  • [ X ]
    Öğe
    Buğday çiminin diyabetli ratlarda bazı hematolojik ve lipit parametrelerine etkisi
    (Young Scholars Union, 2017-08-22) Mis, Leyla; Comba, Bahat; Yeltekin Çilingir, Aslı; Uslu, Sema
    Bu çalışmada deneysel diyabet oluşturulmuş ratlarda buğday çiminin kan parametrelerine ve lipit profili üzerine etkisinin araştırılması amaçlandı. Çalışmada kullanılan ratlar rastgele seçilerek her biri 8 rattan oluşan 4 gruba ayrıldı: Kontrol grubu, tek doz 1 ml fosfat-sitrat tamponu (pH: 4.5) verildi., Diyabet grubu;45 mg/kg tek doz streptozotosin ip enjeksiyon yapıldı., Buğday çimi grubu; ağızdan 10ml/kg/gün 6 hafta buğday çimi ektraktı verildi, Diyabet+Buğday çimi grubu;45 mg/kg tek doz streptozotosin ip enjeksiyon yapıldı ve ağızdan 10ml/kg/gün 6 hafta buğday çimi ektraktı verildi. Deneysel çalışma tamamlanınca 6 haftanın sonunda kan örnekleri alındı.Hematolojik parametreler kan sayım cihazı ile bakıldı. Elde edilen serumlarda trigliserit,kolesterol,LDL, VLDL, UHDL, düzeyleri otoanalizörde analiz edildi.RBC(p? 0.05),HCT(p? 0.05),PLT(p? 0.05),%LY(p? 0.05), değerleri diyabet grubunda düştüğü, Diyabet +Buğday çimi grubunda yükseldiği; WBC(p? 0.05),LYM(p? 0.05),MO(p? 0.05),NEU(p? 0.05),%MO(p? 0.05),%NEU(p? 0.01),düzeylerinin diyabet grubunda yükseldiği Diyabet +Buğday grubunda düştüğü istatistiki olarak belirlendi.Diyabet+buğdayçimi grubunda ise diyabet grubuna göre trigliserit(p? 0.05),VLDL(p? 0.05)düzeylerinin istatistiki olarak önemle azaldığı HDL(p? 0.05) düzeyinin yükseldiği tespit edildi.Serum kolesterol düzeylerinde gruplar arası önem bulunmadı.Sonuç olarak; buğday çiminin STZ ye bağlı diyabette oluşan hematolojik ve bazı serum lipit düzeyindeki bozuklukların düzeltilmesinde etkili olabileceği düşünüldü.Bu değişimlerin açıklanabilmesi için uzun süreli ve moleküler çalışmalar yapılmalıdır.
  • Küçük Resim
    Öğe
    Effect of Wheatgrass on DNA damage, oxidative stress ındex and histological findings in Diabetic Rats
    (Soc Chilena Anatomia, 2018-12) Mis, Leyla; Comba, Bahat; Uslu, Sema; Yeltekin, Aslı
    This study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer injected i.p (pH: 4.5), Diabetes group; 45 mg/kg single dose streptozotocin injected i.p., Wheatgrass group; was given oral wheatgrass (10 ml/kg/day) for 6 weeks, Diabetes +Wheatgrass group; 45 mg/kg single dose streptozotocin injected i.p. and wheatgrass (10 mlikg/day) was given by oral during 6 weeks. After the process of experiment during 6 weeks, blood sample and pancreas tissue were taken. The analysis were done of blood glucose levels, TAS, TOS levels by colorimetric kits; DNA damage by ELISA kits in serum. The pancreas tissues were examined histopathologically. In the group of Diabetes+Wheatgrass was determined that the levels of glucose levels (p<0.001),TOS (p<0.05) and OSI (p<0.01) statistically decreased and heal histopatolojical compared to diabetes group. In the group of Wheatgrass was determined that the levels of TAS p<0.05 statistically increased from other groups. The statistical significance were not found in the level of serum 8OHdG differences between the groups. The beta cells were seen to increase in the group receiving wheatgrass for therapeutic purposes.As a conclusion, it was determined that wheatgrass strengthened the anti-oxidant defense system and reduced the glucose level in diabetic rats.
  • [ X ]
    Öğe
    THE EFFECT OF BARLEY GRASS ON ANTIOXIDANT CAPACITY AND DNA DAMAGE IN RAT WITH RENAL FAILURE
    (Parlar Scientific Publications (P S P), 2017) Comba, Bahat; Mis, Leyla; Uslu, Sema; Comba, Arzu
    This study was aimed to search the effect of barley grass (BG) on the Total Antioxidant (TAS)-Oxidant Status (TOS) and DNA damage (8OHdG) in rat with renal failure. The rats which are used in the study were randomly divided into 4 groups that each of has 8 rats: Control (C) group; injected i.p. with physiological saline once a day for 7 days, GM group; Gentamicin (80 mg/kg/day) was injected i.p. for 7 days, BG group; was given oral BG (250 mg/kg/day) for 4 weeks, GM+BG group was injected gentamicin (80 mg/kg/day) with i.p. for 7 days and BG (250 mg/kg/day) was given by oral for 4 weeks. After from the process of experiment for 4 weeks, blood sample and kidney tissue were taken. The analyses of urea and creatinine were done by autoanalyser; TAS, TOS levels by colorimetric kits; 8OHdG level by ELISA kits in serum. The kidney tissues were examined histologically. In the GM+BG group was statistically decreased levels of urea, creatinine, TOS (p<0.05) and OSI (p<0.01) compared to GM group. In the group of BG was determined the levels of TAS p<0.05 statistically increased other groups. The statistical significance was not found in the level of serum 8OHdG differences between the groups. BG decreased widespread damage to the renal glomerulus and proximal tubulus. As a result, adverse effects of high dose gentamicin application such as kidney damage can be reduced when used barley grass.