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    Effect of chronic exposure to sodium fluoride and 7,12-dimethylbenz[a]anthracene on some blood parameters and hepatic, renal, and cardiac histopathology in rats
    (International Society for Fluoride Research, 2018) Yıldırım, Serkan; Ekin, Suat; Huyut, Zübeyir; Oto, Gökhan; Comba, Arzu; Uyar, Hasan; Şengül, Emin; Çınar, D. Ali
    This study aimed to investigate the effects of both sodium fluoride (NaF) and 7,12-dimethylbenz[a]anthracene (DMBA), both separately and in combination, on some blood parameters and hepatic, renal, and cardiac histopathology in rats. Forty male Wistar albino rats, weighing 250-300 g, were randomly divided into one control and three experimental groups (i) a NaF group who received 15 ppm of NaF in their drinking water for 90 days, (ii) a DMBA group who received 10 mg DMBA/kg body weight/po/ weekly for 90 days, and (iii) a NaF+DMBA group who received 15 ppm NaF in their drinking water plus 10 mg DMBA/kg bw/po/weekly for 90 days. The animals in the groups were sacrificed at the end of the 90 days. The AST, ALT, LDH, CK, creatinine, troponin I, and MDA levels increased in the NaF, DMBA, and NaF+DMBA groups compared to the control group, while the WBC, K, Na, Cl, urea, SOD, GSH-Px, CAT, and GSH values showed a statistically significant decrease (p<0.05). In addition, the CK-MB significantly increased in the DMBA and NaF+DMBA groups compared to the control group (p<0.05). The histological structure of the liver, kidney, and heart tissues in the control group was normal. In the NaF and DMBA groups, degenerative and necrotic changes were detected. In the NaF+DMBA group: (i) the liver exhibited hydropic degeneration and coagulation necrosis in hepatocytes, severe dilation in the sinusoids, congestion in the central and portal regions, and mononuclear cell infiltration in the portal region; (ii) the kidneys displayed congestion in the glomerulus and interstitial vessels, interstitial nephritis, diffuse hydropic degeneration, and coagulation necrosis in the tubule epithelium; (iii) the heart showed myocardial hyperemia, severe mononuclear cell infiltration in interstitial tissue, hyaline degeneration, and Zenker’s necrosis in myocardium As a result of these blood and oxidative stress parameters and histopathological findings, it was determined that NaF, DMBA, and NaF+DMBA induce toxicity in the liver, kidney, and heart tissues and thus play an important role in the physiopathology of toxicity. © 2018, ISFR.
  • Küçük Resim
    Öğe
    How long-term intake of sodium fluoride (NAF) in different doses and 7,12 dimethylbenz(A)anhtracene (DMBA) affect the erythrocyte parameters in rats?
    (Pas Forum, 2019) Comba, Bahat; Oto, Gökhan; Arıhan, Okan; Comba, Arzu; Uyar, Hasan
    This study was aimed to search the effect of Sodium Fluoride (NaF) and 7,12 dimethylbenz(a)anthracene (DMBA) to erythrocyte fragility and parameters in rats. The nine groups were formed and each group contained 8 animals. Group 1: Control group (without any treatment). Group 2: Sesame oil (vehicle for DMBA). Group 3: 1 ppm NaF. Group 4: 15 ppm NaF. Group 5: 30 ppm NaF. Group 6: DMBA.Group 7: 1 ppm NaF + DMBA. Group 8: 15 ppm NaF + DMBA. Group 9: 30 ppm NaF + DMBA. Fluoride was added into the animals’ drinking water in the form of NaF once a day, for 12 weeks. DMBA (10 mg/kg) was administered once a week and in a total of 12 weeks with oral gavage. Erythrocyte fragility was analyzed with osmotic hemolysis method and erythrocyte parameters with blood cell counter in whole blood. At 0.4% NaCl concentration groups 4, 5, 6, 8, and 9 showed significantly higher erythrocyte fragility values than control group (p?0.05). At 0.5% NaCl concentration, groups 6, 8 and 9 showed significant increase in erythrocyte fragility compared to other groups (p?0.05). The erythrocyte and hematocrit values were found significantly high in group 5 (p?0.001) and group 4 (p?0.01) while it was found low in all groups with DMBA (6, 7, 8, 9) (p?0.05) compared to control group. Hemoglobin amount in group 5(p?0.01) and group 4 (p?0.05) were significantly higher than other groups. MCV and MCH in group 5 were significantly lower and these values in all groups with DMBA (6, 7, 8, 9) were determined significantly high compared to other groups. RDWC in group 5 (p?0.001) and group 4 (p?0.01) and in all groups with DMBA (6, 7, 8, 9) (p?0.05) was significantly increased compared to other groups. As a result, exposure to high doses of floride and DMBA may cause augmented erythrocyte fragility, abnormal erythrocyte parameters and anemia. Therefore, measures must be taken to protect the health of all living organisms in area exposed to high levels of fluoride and DMBA.
  • [ X ]
    Öğe
    How Long-term Intake of Sodium Fluoride (NaF) in Different Doses and 7,12 dimetilbenz(a)antrasen (DMBA) Affect Erythrocyte Fragility and Parameters in Rats?
    (Wiley, 2017) Comba, Bahat; Oto, Gokhan; Arihan, Okan; Comba, Arzu; Uyar, Hasan
    [Abstract Not Available]
  • [ X ]
    Öğe
    Ratlarda farklı dozlarda Sodyum Florür (Naf)’ ün ve Dimetilbenz(A)Antrasen (DMBA)’ in uzun süreli alımı Eritrosit kırılganlığını ve parametrelerini nasıl etkiler?
    (Pamukkale Üniversitesi, 2017-09-10) Comba, Bahat; Oto, Gökhan; Arıhan, Okan; Comba, Arzu; Uyar, Hasan
    GİRİŞ-AMAÇ: Bu çalışmada sıçanlarda Sodyum Florür (NaF) ve 7,12 dimetilbenz(a) antrasen (DMBA) ‘nin eritrosit kırılganlığına (EK) ve parametrelerine olan etkisinin araştırılması amaçlandı. YÖNTEM: Dokuz grup oluşturuldu ve her grupta 8 hayvan yer aldı. Grup 1: Kontrol. Grup 2: Susam yağı. Grup 3: NaF1 Grup 4: NaF15. Grup 5: NaF30. Grup 6: DMBA. Grup 7: NaF1 + DMBA. Grup 8: NaF15 + DMBA. Grup 9: NaF30 + DMBA. Hayvanların içme suyuna, günde bir kez NaF (ppm) ve haftada bir kez DMBA (10 mg/kg) 12 hafta gavaj ile uygulandı. EK, spektrofotometrik yöntemle çalışıldı. Veriler Anova ve Duncan testi ile değerlendirildi BULGULAR: % 0.4 NaCl konsantrasyonda EK, Grup 4, 5, 6, 8 ve 9’ da kontrol grubuna göre anlamlı olarak daha yüksekti Grup 5’deki MCV ve MCH, anlamlı olarak en düşüktü ve bu değer DMBA’lı tüm gruplarda (6, 7, 8, 9) diğer gruplara kıyasla belirgin olarak yüksek tespit edildi. RDWC’ de, Grup 5 (p SONUÇ: Sonuç olarak, yüksek dozdaki florür ve DMBA’ya maruz kalma, EK’na, anormal eritrositlere ve anemiye neden olabilir. Bu nedenle, yüksek seviyedeki florür ve DMBA’ya maruz kalan bölgede yaşayan tüm canlıların sağlığını korumak için acil tedbirler alınmalıdır.