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Öğe A serosurvey on some Canine Vector-borne Zoonoses (Anaplasma spp., Ehrlichia spp., Borrelia burgdorferi, Dirofilaria immitis and Leishmania spp.) in Osmaniye(Atatürk Üniversitesi, 2019) Güven Gökmen, Tülin; Günaydın, Elçin; Turut, Nevin; Akın, Bünyamin; Koç, Özgür; Ütük, Armağan ErdemAbstract: Vector-borne diseases in dogs are of major global significance for their impact on animal and human health. Especially, it is necessary to determine the prevalence of agents found in reservoir animals by conventional, molecular and serological methods for the application of control programs for these diseases. Serosurvey studies are one of the reliable methods to know the presence and prevalence of these diseases in our country and region. In this study, it was aimed to detect the prevalence of Ehrlichia canis/E.ewingii, Anaplasma platys/A.phagocytophilum, Borrelia burgdorferi, Dirofilaria immitis and Leishmania infantum in dogs in Osmaniye. Five canine vector-borne diseases were investigated with a rapid in-clinic enzyme-linked immunosorbent assay in 100 clinically healthy dog serum samples in Osmaniye city center, Düziçi, Sumbas, Kadirli, Hasanbeyli, Bahçe and Toprakkale districts. Seroprevalence rate was detected as 3% for E.canis/E.ewingii and 1% for D.immitis by SNAP 4Dx PLUS. The prevalence of A.platys/A.phagocytophilum, Borrelia burgdorferi and L.infantum were determined as 0%. In conclusion, our study in which we determined the seroprevalence of dog vector-borne diseases in Osmaniye is the first study in which five agents are determined in one step and will contribute to the effective control programs prepared for animal and public health in our region.Öğe Effect of chronic exposure to sodium fluoride and 7,12-dimethylbenz[a]anthracene on some blood parameters and hepatic, renal, and cardiac histopathology in rats(International Society for Fluoride Research, 2018) Yıldırım, Serkan; Ekin, Suat; Huyut, Zübeyir; Oto, Gökhan; Comba, Arzu; Uyar, Hasan; Şengül, Emin; Çınar, D. AliThis study aimed to investigate the effects of both sodium fluoride (NaF) and 7,12-dimethylbenz[a]anthracene (DMBA), both separately and in combination, on some blood parameters and hepatic, renal, and cardiac histopathology in rats. Forty male Wistar albino rats, weighing 250-300 g, were randomly divided into one control and three experimental groups (i) a NaF group who received 15 ppm of NaF in their drinking water for 90 days, (ii) a DMBA group who received 10 mg DMBA/kg body weight/po/ weekly for 90 days, and (iii) a NaF+DMBA group who received 15 ppm NaF in their drinking water plus 10 mg DMBA/kg bw/po/weekly for 90 days. The animals in the groups were sacrificed at the end of the 90 days. The AST, ALT, LDH, CK, creatinine, troponin I, and MDA levels increased in the NaF, DMBA, and NaF+DMBA groups compared to the control group, while the WBC, K, Na, Cl, urea, SOD, GSH-Px, CAT, and GSH values showed a statistically significant decrease (p<0.05). In addition, the CK-MB significantly increased in the DMBA and NaF+DMBA groups compared to the control group (p<0.05). The histological structure of the liver, kidney, and heart tissues in the control group was normal. In the NaF and DMBA groups, degenerative and necrotic changes were detected. In the NaF+DMBA group: (i) the liver exhibited hydropic degeneration and coagulation necrosis in hepatocytes, severe dilation in the sinusoids, congestion in the central and portal regions, and mononuclear cell infiltration in the portal region; (ii) the kidneys displayed congestion in the glomerulus and interstitial vessels, interstitial nephritis, diffuse hydropic degeneration, and coagulation necrosis in the tubule epithelium; (iii) the heart showed myocardial hyperemia, severe mononuclear cell infiltration in interstitial tissue, hyaline degeneration, and Zenker’s necrosis in myocardium As a result of these blood and oxidative stress parameters and histopathological findings, it was determined that NaF, DMBA, and NaF+DMBA induce toxicity in the liver, kidney, and heart tissues and thus play an important role in the physiopathology of toxicity. © 2018, ISFR.Öğe How long-term intake of sodium fluoride (NAF) in different doses and 7,12 dimethylbenz(A)anhtracene (DMBA) affect the erythrocyte parameters in rats?(Pas Forum, 2019) Comba, Bahat; Oto, Gökhan; Arıhan, Okan; Comba, Arzu; Uyar, HasanThis study was aimed to search the effect of Sodium Fluoride (NaF) and 7,12 dimethylbenz(a)anthracene (DMBA) to erythrocyte fragility and parameters in rats. The nine groups were formed and each group contained 8 animals. Group 1: Control group (without any treatment). Group 2: Sesame oil (vehicle for DMBA). Group 3: 1 ppm NaF. Group 4: 15 ppm NaF. Group 5: 30 ppm NaF. Group 6: DMBA.Group 7: 1 ppm NaF + DMBA. Group 8: 15 ppm NaF + DMBA. Group 9: 30 ppm NaF + DMBA. Fluoride was added into the animals’ drinking water in the form of NaF once a day, for 12 weeks. DMBA (10 mg/kg) was administered once a week and in a total of 12 weeks with oral gavage. Erythrocyte fragility was analyzed with osmotic hemolysis method and erythrocyte parameters with blood cell counter in whole blood. At 0.4% NaCl concentration groups 4, 5, 6, 8, and 9 showed significantly higher erythrocyte fragility values than control group (p?0.05). At 0.5% NaCl concentration, groups 6, 8 and 9 showed significant increase in erythrocyte fragility compared to other groups (p?0.05). The erythrocyte and hematocrit values were found significantly high in group 5 (p?0.001) and group 4 (p?0.01) while it was found low in all groups with DMBA (6, 7, 8, 9) (p?0.05) compared to control group. Hemoglobin amount in group 5(p?0.01) and group 4 (p?0.05) were significantly higher than other groups. MCV and MCH in group 5 were significantly lower and these values in all groups with DMBA (6, 7, 8, 9) were determined significantly high compared to other groups. RDWC in group 5 (p?0.001) and group 4 (p?0.01) and in all groups with DMBA (6, 7, 8, 9) (p?0.05) was significantly increased compared to other groups. As a result, exposure to high doses of floride and DMBA may cause augmented erythrocyte fragility, abnormal erythrocyte parameters and anemia. Therefore, measures must be taken to protect the health of all living organisms in area exposed to high levels of fluoride and DMBA.Öğe Some mineral substance, oxidative stress and total antioxidant levels in Norduz and Morkaraman sheep(Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi, 2018-10-09) Mis, Leyla; Mert, Handan; Comba, Arzu; Comba, Bahat; Doğan Söğütlü, İnci; Irak, Kıvanç; Mert, NihatThe aim of the study was to compare some mineral substances and total antioxidant and totaloxidan levels of healthy Morkaraman and Norduz sheep breeds in the same age and care conditions. A total of 20 animals, 10 from each sheep breed, were included in the study. Serum total oxidant status (TOS) and total antioxidant status (TAS) were determined by a colorimetric method. OSI value was determined by calculating the TASTOS ratio. When the two sheep were compared, there was a difference between TAS, Mg, P levels. Antioxidant levels of Norduz sheep were higher than morkaraman sheep. The given data may be the reference value for studies of these sheep breedsÖğe Karagül ve Norduz koyunlarında bazı hematolojik ve biyokimyasal parametreler(Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi, 2017-10-24) Comba, Bahat; Mert, Handan; Comba, Arzu; Mis, Leyla; Mert, NihatBu çalışma da aynı yaş, bakım ve besleme şartlarında sağlıklı Karagül ve Norduz koyun ırklarının bazı hematolojik ve biyokimyasal parametrelerinin karşılaştırılması amaçlandı. Her bir koyun ırkından 15 adet olmak üzere toplam 30 hayvan çalışmaya dahil edildi. Tam kan alyuvar sayısı (RBC), hemoglobin (HGB), hematokrit (HCT), ortalama alyuvar hacmi (MCV), ortalama alyuvar hemoglobini (MCH), ortalama alyuvar hemoglobin konsantrasyonu (MCHC), alyuvarların dağılım sıklığı (RDWC), akyuvar (WBC), lenfosit (LYM), monosit (MON), granülosit (GRN) sayıları, lenfosit (LY), monosit (MO), granülosit (GR) yüzdeleri, trombosit sayısı (PLT), trombosit yüzdesi (PCT), ortalama trombosit hacmi (MPV), trombosit dağılım sıklığı (PDWC) gibi hematolojik parametreleri kan sayım cihazında; serum alkalen fosfataz (ALP), aspartat aminotransferaz (AST), alkali fosfataz (ALP), gamma-glutamil transpeptidaz (GGT), total protein (TP) albümin (A), globulin (G) total bilirubin (TB) indirekt bilirubin (İB), kreatin, kreatin kinaz (CK), kreatin kinaz MB (CK-MB), üre, ürik asit (UA), kan üre azotu (BUN) ve amilaz biyokimyasal parametreleri oto analizörde belirlendi. HCT (p<0.001), MCV (p<0.001) ve MCH (p<0.001), CK (p<0.05) ve amilaz (p<0.001) Karagül koyun ırkında; LYM (p<0.05), RDWC (p<0.001), PLT (p<0.001), PCT (p<0.001), TP (p<0.05) ve IB (p<0.001) ise Norduz koyun ırkında daha yüksekti. Diğer parametrelerdeki değişimler önemsizdi. Sonuç olarak, Karagül ve Norduz koyun ırklarına ait bazı hematolojik ve biyokimyasal parametrelerdeki farklılıklar ırklara ait bir özellik olabilir. Elde edilen verilerin özellikle Veteriner Hekimlere, bu koyun ırklarına ait hastalıkların erken tanısında ve tedavisinde, prognozunda ve de bu ırklar ile ilgili yapılacak araştırmalarda referans olabileceği kanısındayız.Öğe The effect of chemical and surgical castration on the live weight gain and some hormones of male Norduz sheep(Zoological Socety of Pakistan, 2017-10) Karakuş, Kadir; Comba, Bahat; Taş, Abuzer; Sancak, Tunahan; Comba, Arzu; Sarıpınar Aksu, Devrim; Koyun, Hasan; Tariq, Mohammad MasoodThe aim of this study was to investigate the effects of surgical and chemical castration on body weight and some hormones. In the present study, 2.5-3-month-old weaned male Norduz sheep were used. The sheep were obtained in the year 2015 from Livestock Application and Research Directorate of Yuzuncu Yil University. The sheep were randomly allocated into three groups each of which contained 10 sheep. The groups were formed as; Group 1 The Control Group (No castration), Group 2 (Surgical castration group) and Group 3 (Chemical castration group), respectively. Blood sample was taken from vena jugularis of the animals before the application and every two months for a total of 4 times for 6 months and hormone analyses were performed. The order of statistical significance for TSH (thyroid stimulating hormone) were found as Surgical > Control > Formol (P<0.05). The order of statistical significance for growth hormone was Formol = Surgery > Control (P<0.05). In August month, the application factor was found to be significant only in testosterone (P<0.05). No significant difference between the control and surgical groups in testosterone was noted. In this study, although the application of different castration methods in 4-month-old male Norduz sheep resulted in changes in the blood hormone levels, it was determined that these changes did not affect body weight. In conclusion, the evaluation of the process in castrated six-month-old sheep revealed an increase in the body weight which was similar to that in the control group.Öğe The first detection of anti-anaplasma phagocytophilum antibodies in horses in Turkey(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2018) Günaydın, Elçin; Pekkaya, Selçuk; Kuzugüden, Fatih; Zeybek, Melis; Güven Gökmen, Tülin; Ütük, Armağan ErdemAnaplasma phagocytophilum, the causative agent of equine granulocytic anaplasmosis, affects several species of wild and domesticated mammals, including horse, besides human. In Turkey, there were many reports on A. phagocytophilum circulation among cattles, sheep, dogs, mice, humans, except horses. In this study, we aimed to inquiry whether A. phagocytophilum were circulating among the horse population or not. For this purpose, 105 mare horse blood sera were examined for the presence of Anti-Anaplasma phagocytophilum IgG antibodies by IFAT. The seroprevalance rate of 8.57% horse sera were found to be positive. This was the first report about the presence of anti-A. phagocytophilum antibodies in horses in Turkey..Öğe Effect of Wheatgrass on DNA damage, oxidative stress ındex and histological findings in Diabetic Rats(Soc Chilena Anatomia, 2018-12) Mis, Leyla; Comba, Bahat; Uslu, Sema; Yeltekin, AslıThis study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer injected i.p (pH: 4.5), Diabetes group; 45 mg/kg single dose streptozotocin injected i.p., Wheatgrass group; was given oral wheatgrass (10 ml/kg/day) for 6 weeks, Diabetes +Wheatgrass group; 45 mg/kg single dose streptozotocin injected i.p. and wheatgrass (10 mlikg/day) was given by oral during 6 weeks. After the process of experiment during 6 weeks, blood sample and pancreas tissue were taken. The analysis were done of blood glucose levels, TAS, TOS levels by colorimetric kits; DNA damage by ELISA kits in serum. The pancreas tissues were examined histopathologically. In the group of Diabetes+Wheatgrass was determined that the levels of glucose levels (p<0.001),TOS (p<0.05) and OSI (p<0.01) statistically decreased and heal histopatolojical compared to diabetes group. In the group of Wheatgrass was determined that the levels of TAS p<0.05 statistically increased from other groups. The statistical significance were not found in the level of serum 8OHdG differences between the groups. The beta cells were seen to increase in the group receiving wheatgrass for therapeutic purposes.As a conclusion, it was determined that wheatgrass strengthened the anti-oxidant defense system and reduced the glucose level in diabetic rats.
