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Downregulation of HSP27 by isoindole-derived pyrrolidines suppressing multidrug resistance (MDR) and inducing apoptosis in MCF-7 and DLD-1 cell lines
(NATL INST SCIENCE COMMUNICATION-NISCAIR, 2024) Mesci, Seda; Yazgan, Burak; Gül, Melek; Yıldırım, Tuba
In most cancer treatments, major problem arises from the prevention of cell death (apoptosis suppression) with the development of drug resistance. Anticancer agents that ensure elimination of drug resistance and drug-resistant cells to apoptosis, are among the main targets. Here, we evaluated a series of synthesized N-phenyl maleimide substituents in tetracyclic compounds as anticancer drug candidate. We selected compounds may lead to death and eliminate drug resistance in breast and colon cells. In MCF-7 and DLD-1 cell lines; multidrug resistance genes (ABCB1, ABCC3, ABCC10, ABCC11 and ABCG2), apoptosis mechanism genes (BAX, BCL-2, p53, PARP and CASP3), heat shock genes (HSP27, HSP40, HSP60, HSP70 and HSP90?) and endoplasmic reticulum (ER) chaperone genes (GRP78 and GRP94) mRNA levels were determined by qPCR method. Amounts of proteins of apoptosis and signalling pathways were measured by human apoptosis antibody array. The compounds have been shown to have downregulation on multidrug resistance genes other than ABCC3. It was found that all compounds in MCF-7 and DLD-1 cells showed significant increase in p53, BAX and CASP3 gene expressions. Also, the compounds have the potential to reduce gene expression of heat shock genes (HSPs). While the compounds have been determined to increase protein expression in BAD, BAX, BID, BIM, Caspase-3, Caspase-7, Caspase-8, Cytochrome-C, Fas, TNF, TRAIL, p27, p38 and p53; decrease protein expression in AKT, BCL-2, ERK1/2, HSP27, HSP60, IGFs, JNK, NFKB, PARP, TAK1, Survivin in MCF-7 and DLD-1 cells. The compounds stand out with their inhibition of HSP27 in DLD-1 cells and their inhibition with HSP27 and NFkB in MCF-7 cells. Overall, it has been shown that these compounds increase intrinsic and extrinsic proapoptotic proteins, decrease antiapoptotic proteins, decrease HSPs and some growth factors, and they may serve as potential anticarcinogenic molecules.
From Captivity to Liberty: A Study on the Prison Writings of Martin L. King, Ngugi and Soyinka
(Rector CIU Cyprus Int Univ, 2024) Bolat, Eren; Ekler, Onur
Prisons, like other disciplining apparatuses of the state, are used to reform the prisoners so that they can be re-conditioned back to a set of pre-ordained roles designed in the system. These are places hostile to individuality, freedom, and creativity. They function like rehabilitating institutes to suppress the reactionary or rebellious voices of the prisoners by effacing their individuality under harsh conditions so that they can make them docile bodies. However, this objective fails when the prisoner-intellectuals are of concern. For them, prisons become their shrines where they are overwhelmed by the transformative power of imprisonment. The experience of confinement shapes their perspectives, deepens their commitment to social justice, and fuels their advocacy for change. Although the physical conditions of the prisons hamper their urge to write, they never give up writing. Some write on the prison walls and some on toilet papers. Despite such horrible conditions, they manage to produce their most influential works which can be classified in prison literature. The writings of these prisoner-writers have paved the way for the liberation of colonized/discriminated people in their own countries and in diverse diasporas. Martin L. King’s Letter from Birmingham Jail (2018), Ngugi wa Thiong’o’s Detained: A Writer’s Prison Diary (1981) and Wole Soyinka’s The Man Died: The Prison Notes (1988) are three significant works to be featured in this genre. Through an interdisciplinary approach, this article identifies the distinctive elements and commonalities in the prison writings of King, Ngugi, and Soyinka. This study explores the universal concepts of incarceration, resistance to oppressive systems, and the struggle for freedom as portrayed in the works of these writers, and aims to examine how these writers have transformed their works into tools of resistance.
Characterization of p(PmO), p(LO) and p(RO) organoparticles, their bioactivity properties and their effect on pancreatic cancer Capan-1 cell
(ELSEVIER SCIENCE SA, 2023) Alpaslan, Duygu; Turan, Abdullah; Ersen Dudu, Tuba; Moran Bozer, Büşra; Aktaş, Nahit; Türk, Mustafa
For the first time in the literature, p (PmO), p (LO) and p (RO) organo-particles were synthesized from Peppermint oil, Lemon oil and Rose oil. Of the organo-particles L-929 cell line viability/cytotoxicity and anticancer effect against Capan-1 pancreatic cancer cell line were investigated. p (PmO), p (LO) and p (RO) organo-particles were featured by thermogravimetry (TGA), Fourier Transform Infrared Spectroscopy (FTIR), Scanning electron microscope (SEM), Particle size (DLS), and particle charge (zeta potential, Zeta) analyses. Antioxidant, biocompatible, and antimicrobial activities and in vitro cytotoxicity specialties were investigated. In studies on Capan-1 and L-929 cell lines, it was observed that p (PmO), p (LO) and p (RO) organo-particles were effective on L-929 fibroblast cell line on Capan-1 pancreatic cancer cell line. In addition, it was observed that p (PmO), p (LO) and p (RO) organo-particles were not toxic in L-929 cell lines at high doses. When the Capan-1 cell line MTT analysis results of p (PmO), p (LO) and p (RO) organo-particles were examined, a difference was observed between cell viability rates and apoptosis and necrosis values. The highest % apoptosis rate was observed in the p (RO) organo particle.
Resorbable membrane design: In vitro characterization of silver doped-hydroxyapatite-reinforced XG/PEI semi-IPN composite
(ELSEVIER, 2023) Doğan, Deniz; Erdem, Ümit; Moran Bozer, Büşra; Türköz, Mustafa Burak; Yıldırım, Gürcan; Metin, Ayşegül Ülkü
In this study, the production and characterization of silver-doped hydroxyapatite (AgHA) reinforced Xanthan gum (XG) and Polyethyleneimine (PEI) reinforced semi-interpenetrating polymer network (IPN) biocomposite, known to be used as bone cover material for therapeutic purposes in bone tissue, were performed. XG/PEI IPN films containing 2AgHA nanoparticles were produced by simultaneous condensation and ionic gelation. Characteristics of 2AgHA-XG/PEI nanocomposite film were evaluated by structural, morphological (SEM, XRD, FT-IR, TGA, TM, and Raman) and biological activity analysis (degradation, MTT, genotoxicity, and antimicrobial activity) techniques. In the physicochemical characterization, it was determined that 2AgHA nanoparticles were homogeneously dispersed in the XG/PEI-IPN membrane at high concentration and the thermal and mechanical stability of the formed film were high. The nanocomposites showed high antibacterial activity against Acinetobacter Baumannii (A.Baumannii), Staphylococcus aureus (S.aureus), and Streptococcus mutans (S.mutans). L929 exhibited good biocompatibility for fibroblast cells and was determined to support the formation of MCC cells. It was shown that a resorbable 2AgHA-XG/PEI composite material was obtained with a high degradation rate and 64% loss of mass at the end of the 7th day. Physico-chemically developed biocompatible and biodegradable XG-2AgHA/PEI nanocomposite semi-IPN films possessed an important potential for the treatment of defects in bone tissue as an easily applicable bone cover. Besides, it was noted that 2AgHA-XG/PEI biocomposite could increase cell viability, especially in dental-bone treatments for coating, filling, and occlusion.
Photocrosslinked methacrylated pectin and methacrylated hyaluronic acıd wound dressing loaded with oleuropein as bioactive agent
(TAYLOR & FRANCIS AS, 2023) Moran Bozer, Büşra; Özkahraman, Bengi; Mert Balaban, Hümeyra
Oleuropein-loaded photocrosslinked hydrogels, consisting of methacrylated pectin (PEC-MA) andmethacrylated hyaluronic acid (HA-MA), were developed as a new hydrogel wound dressing.Pectin and hyaluronic acid were methacrylated and the composite hydrogels were developed byphotocrosslinking of the methacrylated polymer precursors. Methacrylation and the photocros-slinking reactions were approved by1H-NMR and FTIR analyses. In addition to thermal and mor-phological characterizations, swelling andin vitrodegradation studies were performed. Moreimportantly, bioactivity studies, such as cytotoxicity, genotoxicity and hemolysis were performedto reveal the potential of the oleuropein-loaded photocrosslinked hybrid hydrogels for wounddressing applications.In vitroscratch assay studies showed that oleuropein-loaded wound dressingeffectively promoted the wound healing compared to the control in 24 hrs. Oleuropein-loadedphotocrosslinked hydrogels had no genotoxic and cytotoxic effects on L929 cells and could havea potential for wound healing applications.
Synthesis of Silver Nanoparticles From Camellia sinensis Waste and Evaluation of Their Cytotoxic Effect
(Gh. Asachi Technical University of Iasi, 2023) Erenler, Ramazan; Tunay, Karan; Moran Bozer, Büşra
Silver nanoparticle synthesis was achieved using waste tea produced from tea factories and investigated their anticancer activity. The waste material was heated with deionized water at 45°C and then the solution was filtrated. The filtrate was reacted with silver nitrate to yield the silver nanoparticles (AgNPs). The structure of AgNPs was determined by spectroscopic analysis. The cytotoxic effect of extract and AgNPs were performed using the human lung carcinoma cell lines (A549), colon adenocarcinoma cell lines (DLD-1), and mouse fibroblasts (L929) cell lines by MTT assay. In UV-Vis measurement, the maximum absorption was observed at 445 nm. The nanoparticles morphology was established by Transmission electron microscopy (TEM) and particle size was determined as 17.6 nm. The structure of AgNPs was presented as a face-centered cubic structure by X-ray Diffraction (XRD) analysis. The high negative value of zeta potential (-31.3 mV) displayed the stability of the nanostructure. The yield of AgNPs was calculated by elemental analysis as 72.35%. AgNPs exhibited an outstanding effect on A549 and DLD-1 cell lines at 1.0 mg/mL with the lethal effect of 89.3% and 83% respectively. AgNPs synthesized from waste tea may be promising agents for cancer treatment. Since silver nanoparticles synthesized from waste tea revealed excellent cytotoxic effects against various cancer cells. © 2023 Gheorghe Asachi Technical University of Iasi, Romania. All rights reserved.
p(thyme oil) and p(clove oil) organo-particles with biocompatible, anticancer, antioxidant, and antibacterial properties against Capan-1 and L-929 cells
(WILEY, 2023) Alpaslan, Duygu; Ersen Dudu, Tuba; Moran Bozer, Büşra; Aktaş, Nahit; Türk, Mustafa
The synthesis of p(ClO) and p(TO) organo-particles from clove oil and thyme oil is the first in the literature. The particles were tested against the L-929 cell line for cell viability/cytotoxicity. The anticancer activity was studied against the Capan-1 pancreatic cancer cell line. p(ClO) and p(TO) organo-particles were featured by thermogravimetry (TGA), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), particle size (DLS), and particle charge (zeta potential, Zeta) analyses. Antioxidant, biocompatible, antimicrobial, and in vitro cytotoxicity specialties were investigated. p(ClO) and p(TO) organo-particles were found to be effective on the L-929 fibroblast cell line and Capan-1 pancreatic cancer cell line in research on Capan-1 and L-929 cell lines. Additionally, it was shown that large dosages of p(ClO) organo-particles were not hazardous to L-929 cell lines. A difference was found between the rates of cell viability and apoptosis and necrosis when the MTT study findings of p(ClO) and p(TO) organo-particles were studied in Capan-1 cell line. The p(TO) organo-particle had the highest % apoptosis rate. At the 100?g?mL?1 concentration, the fibroblast cell viability of p(ClO) and p(TO) organo-particles was 176.46% and 107.78%, respectively. The IC50 value derived for the decrease in viability was determined as (2.22?mg?mL?1) and it was calculated that it would kill the pancreatic cancer cells by 50% when doxorubicin and p(ClO) were administered combined.
TEMSİLİ DEMOKRASİDEN, DOĞRUDAN DEMOKRASİYE YÖNELİM: TÜRKİYE’DE CUMHURBAŞKANI ADAYLARININ BELİRLENMESİNDE 100 BİN İMZA ÖRNEĞİ VE TARAFSIZLIĞINA YÖNELİK BİR DEĞERLENDİRME
(Cenay Babaoğlu, 2021) Güvenç, Cemaleddin
Demokrasi kuramı ve pratiği 19. yüzyılda gelişen, 20. Yüzyılda olgunlaşan ve 21. yüzyılda da yeni anlam ve özü kazanan bir kavram olarak karşımıza çıkmaktadır. Halkın, halk tarafından yönetimi olarak kabul edilen demokrasi, seçimleri mecburi kılarken yönetimin de meşruluğunu seçimlere dayandırmaktadır. Türkiye Cumhuriyeti kurulduğu 1923 yılından 2020 yılına kadar 21 kez cumhurbaşkanı seçimi yaşanmış, bunların 18’inde cumhurbaşkanı temsili demokrasinin aracı olan TBMM tarafından seçilmiştir. 2007 ve 2017 yıllarında yapılan Anayasal değişikliklerle beraber 2014 ve 2018 yılında yapılan cumhurbaşkanı seçimlerinde ise doğrudan demokrasinin aracı olan halk tarafından doğrudan doğruya cumhurbaşkanı seçilmiştir. Bu çalışmada ilk olarak demokrasi kavramı ve uygulanışı bakımından türlerinden bahsedilmiş, Türkiye Cumhuriyeti Anayasalarında cumhurbaşkanı seçimlerine değinilmiş, ardından 1982 Anayasası ile beraber cumhurbaşkanı adaylarının belirlenmesine yönelik değişiklikler aktarılmıştır. 2017 yılında yapılan Anayasal değişiklikle beraber cumhurbaşkanının belirlenmesine yönelik aday olabilme kıstasları değişerek, seçmenler tarafından 100 bin imza ile de cumhurbaşkanlığına aday gösterilebileceği düzenlemesi getirildiği gibi cumhurbaşkanının da partili olmasının yolu açılmıştır. Çalışmada yeni sistemde cumhurbaşkanı adaylarının belirlenmesinde partili olmayan ve 100 bin imza koşulunun mecburi olmasına yönelik tez öne sürülmüş ve Cumhurbaşkanlığı Kararnameleri ve Anayasa üzerinden analizleri yapılmıştır. Sonuç olarak; Cumhurbaşkanı adayların yeni hükümet sisteminde güçlü hükümet, güçlü meclis ve güçlü yargı ile beraber denge-denetim-fren sisteminin Türk demokrasisine ve Cumhurbaşkanlığı Hükümet sistemine daha işlerlik kazandıracağı yönlü tez ile ortaya koyulmaya çalışılmaktadır.
FRANSIZ BURJUVA DEVRİMİ: BİR AYDINLANMA HAREKETİ OLARAK MUTLAK MONARŞİDEN CUMHURİYETE GEÇİŞ
(Kayseri Üniversitesi, 2020) Güvenç, Cemaleddin
Bu çalışmada Aydınlanma dönemi, Fransız aydınlanması ve Fransız devrimi üzerinden yaşanan olaylar tarihsel bağlamdan kopmadan ele alınacaktır. Şöyle ki 17.yüzyılda Descartes'in ortaya çıkardığı yeni fikrî akımı olan akılcılık tüm Avrupa'yı derinden etkilemiştir. 18.yüzyılda cereyan eden sonuçları bakımından da günümüze kadar etkisini gösteren, 1688 İngiliz devrimi ile başlangıcını oluşturan ve 1789 Fransız Devrimi ile de doruk noktasına ulaşan bir devri ve bu devirde Batı dünyasında bilim ve felsefi keşifler sosyal ve siyasal süreçler bir bütün olarak kastedilmektedir. Tüm gelişmeler Aydınlanmayla beraber değerlendirildiğinde Fransa’da daha radikal olarak adlandırılabilecek gelişmelerin meydana çıkmasına sebebiyet vermiştir. Çalışmanın ışığı altında şu aşamalardan geçilmesi hedeflenmiştir. Bu merhaleler şunlardır; Fransız devrimine giden yolda Voltaire, Rousseau, Condorcet, Montesquieu ve Diderot’un düşünceleri en başta gelmektedir. 18.yüzyıldaki politika, felsefe ve din konularındaki fikirlerin önemli yansıması olarak kabul gören Fransız aydınlanmasının başyapıtı olan Ansiklopedi de bu düşünürlerin ortak fikirlerinde birleşmesinden doğmuştur. Ortak fikirleri Mutlak Monarşi ve Kilisenin despot zorbacı yönetimi ve baskıları, halkın bu yönetim anlayışına karşı isyanı ve başkaldırısındaki haklılıkları ele alınacaktır. Çalışmanın sonunda konular, düşünürler ve olaylar üzerinden derinlemesine ele alınarak, nihayetinde Fransız Devrim ertesi kan dökücüleri olan Jakobenlerden de bahsedilerek Mutlak Monarşiden Cumhuriyete giden yolda kanlı taşların nasıl döşendiği, düşünce dünyamıza yaşanan bu devrimin bir kazanç mı zarar mı olduğu üzerinde bir analiz yapılarak öneriler sunulacaktır.
Sosyal Bilimlerde Yöntem Olarak Pozitivizm Bakış Açısı ve Değerlendirmesi
(Bayburt Üniversitesi, 2020) Güvenç, Cemaleddin
Sosyal bilimler, insanın kültürel, sosyal, fiziki ve ekonomik yönünü konu alan ve insan toplumunu ilgilendiren her olayı inceleyen ve bunu derinlemesine ele alan bir bilim dalı olarak karşımıza çıkmaktadır. Sosyal Bilimlerin varlığı çok daha önceki yüzyıllara dayanmakta ise de 19. yüzyılda felsefeden ayrılarak bilim dünyasında kendi bağımsızlığını ilan ettiğini söyleyebiliriz. Bu bilim dalının konusu insanı ve toplumu ilgilendirir. Bu itibarla günümüze kadar ulaşmasında bilgeliğin mirasçılığını da sosyal bilim yapmıştır. İlk önce İngiltere'de başlayan toplumsal değişim Fransa'da özgürlük hareketiyle devam etmiş, daha sonra ise Almanya'da tüm dünyayı etkileyecek modernleşme hareketine dönüşmüştür. Bu değişim ve dönüşümler fikir adamlarını toplumsal olayların çözümünde arayış içerisine itmiştir. Bu toplumsal olaylarla gün yüzüne çıkan pozitivizmi, sistemsel bir bilim düşüncesi haline getiren Auguste Comte'dir. Bilginin deney ve gözlem yoluyla kesinlik elde edilebileceğini belirten Comte, metafiziği bu toplumsal olayların asıl sebebi olarak gördüğünden bilimin dışına itmiştir. Pozitivizm, olgusal sınanabilirlik içeren bilgisi, nesnel ve bilimsel sayılan yönüyle bilgi kuramına dayanması, nomolojik açıklama yöntemi ve bütüncü mantığı bağlamında ele alması yönüyle amprizmin yöntemlerini ele alır. Bununla beraber pozitivist düşünceden etkilenen mantıkçı pozitivistler de bilginin doğrulanabilmesiyle ve anlamlı olmasıyla elde edilebileceği üzerinde durmuşlardır. Ardından Karl Popper, mantıkçı pozitivistlere karşı çıkarak bilginin doğrulanmasının ve anlamlı olmasının yeterli olmadığını belirtmiştir. Yanlışlama ilkesi ile bilginin doğrulanmasından ziyade yanlışlanabildiği, sınırlanabildiği ölçüde kesinlik kazanacağını belirterek pozitivizme ayrı bir boyut kazandırmıştır. Pozitivizm ile birlikte sosyal bilimler alanında bir çığır açılarak, sosyal bilimlere bilimsel nitelik kazandırdığı görülmektedir.
Influence of Responsible Leadership on Inclusive Organizations: A Mixed- Method Study
(CANADIAN INST KNOWLEDGE DEVELOPMENT - CIKD, 2023) Bhatti, Omar Khalid; Irfan, Muhammad; Öztürk, Ali Osman
The ever-increasing diversity in organizations demands deliberate efforts to include the members in organizational activities. Responsible leadership, a blend of corporate social responsibility, ethics, and leadership, can generate willing cooperation and an urge to fully participate, which is pivotal to the success of the inclusive organization. This study explores the influence of responsible leadership on inclusive organization and uncovers its domains and pathways. Sequential mixed methods design has been adopted using both qualitative and quantitative approaches. Qualitative data comprise 25 semi-structured in-depth interviews, and quantitative data 800 responses on questionnaires, collected from eight organizations, four each from the manufacturing and services sectors. It has been found that responsible leadership significantly influences the inclusive organization. The influence is exerted in social and ethical domains and follows five different pathways within each domain. It is also concluded that a responsible leadership style is better suited for inclusive organizations.
Acesulfame complex compounds of some lanthanide group metal cations. Synthesis and characterization
(Elsevier, 2021) Zeybel, Leriman; Köse, Dursun Ali
Some rare earth metal cations (Eu3+, Tb3+, HO3+ , Er3+ and Yb3+) of the acesulfame ligand and complex compounds were synthesized by hydrothermal method. Elemental analysis, infrared spectroscopy, mass analysis and solid-state UV-vis spectroscopy methods were used for the structural characterization of these compounds. In addition, TGA curves were recorded to determine the thermal degradation steps and possible degradation products of complex structures. As a result of the analysis of the structures, it was determined that the load equivalence of the metal cations having +3 oxidation steps provided with three monoanionic acesulfame ligands included in the structure. Rare earth elements, which have high coordination due to their large radii, are thought to exhibit hexadecimal coordination in synthesized molecules. The coordination sphere is complemented by two acesulfame and 2 aqua ligands showing acidic -N and carbonyl oxygen binding to the bidentate. One acesulfame ligand is thought to be located outside the coordination sphere as a counter ion. According to the recorded TGA curves, all molecules were found to contain one hydrate water. It was determined by FT-IR analysis that all complex structures formed the metal oxides in the reaction vessels as thermal decomposition products. (C) 2020 Elsevier B.V. All rights reserved.
Novel mixed ligand coordination compounds of some rare earth metal cations containing acesulfamato/N,N-diethylnicotinamide
(Scientific Technical Research Council Turkey-Tubitak, 2021) Zeybel, Leriman; Köse, Dursun Ali
The mixed ligand coordination compounds containing acesulfamato and N,N-diethylnicotinamide biomolecules of some rare earth metal cations (Eu3+, Tb3+, Ho3+, Er3+ and Yb3+) were synthesized, and their structural properties were investigated. Possible structural formulas have been proposed by determining the chemical composition of molecules (elemental analysis), binding properties (infrared spectroscopy, mass analysis, solid-state UV-vis spectroscopy), thermal degradation properties (TGA / DTA curves). Based on the data collected, it is suggested that rare earth metal cations with a 3+ oxidation state have sextet coordination. The geometries of the structures were thought to be distorted octahedral. The charge balance of the coordination sphere is balanced by a monoanionic acesulfamato located outside the coordination sphere. When the thermal behaviours of the complexes were examined, it was determined that the compounds with Eu3+, Tb3+, and Yb3+ metal cations contained one hydrate water outside the coordination sphere. Hydrate waters do not exist in the Ho3+ and Er3+ metal cation-centred complexes. At the end of the thermal decomposition analysis of all complex structures, it was determined that they leave the relevant metal oxides in the reaction vessels as final decomposition products.
Reducing lactose content of milk from livestock and humans via lactose imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-aspartic acid) cryogels
(Walter De Gruyter Gmbh, 2021) Erol, Kadir; Arslan Akveran, Gönül; Köse, Kazım; Köse, Dursun Ali
Lactase, which can cause lactose intolerance in its deficiency, is a vital enzyme concerning digestion. To overcome lactose intolerance for patients with digestion problem depending of this kind of issue, lactose in food should be removed. In this study, lactose imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-aspartic acid), poly(HEMA-MAsp), cryogels were synthesized to reduce the amount of lactose content of milk samples. Occurrence of desired bounds, structural integrity, and surface characteristics were analyzed via Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), scanning electron microscope (SEM), micro computed tomography (CT), and confocal microscope methods. Water retention characteristic were tested in solution with different electrolytic nature. Adsorption parameters were optimized in an aqueous medium. The adsorption performance of imprinted cryogels was studied in milk samples obtained from cow, sheep, goat, buffalo, and from human volunteers at different intervals after birth. Amount of lactose adsorbed in aqueous media and milk sample from humans were 322 (56.7%) and 179.5 (5.94%) mg lactose/g polymer, respectively. Selectivity studies revealed an approximately 8-fold increase in adsorption rate of molecularly imprinted cryogels as compared to that of nonimprinted cryogels. In addition, competitive adsorption was conducted using lactose-imprinted cryogels in aqueous media containing lactose, glucose, and galactose molecules resulting in adsorption rates of 220.56, 57.87, and 61.65 mg biomolecule/g polymer, respectively.
Expressions of glutathione S-transferase alpha, mu, pi, and theta in the skin samples of patients with acne rosacea
(Wiley, 2020) Takcı, Zennure; Güneş Bilgili, Serap; Kılıç, Murat; Oğuztüzün, Serpil; Moran Bozer, Büşra; Şimşek, Gülçin Güler; Karadağ, Ayşe Serap
Background Data point to the importance of oxidative stress in rosacea. Glutathione S-transferases (GSTs) have substantial roles in a wide variety of oxidative stress-related conditions. Aim To evaluate the immunohistochemical staining characteristics of GST alpha (GSTA), mu (GSTM), pi (GSTP), and theta (GSTT) in patients with rosacea. Patients/Methods The study included 23 women and 7 men with rosacea (mean +/- SD age 49 +/- 11 year) and 15 healthy control subjects (10 women, 5 men; mean +/- SD age 47.86 +/- 10.88 year). For each patient, the average disease duration, disease subtype, ocular involvement, and severity score were recorded. A 3-mm punch biopsy was taken from the facial skin of each patient and control. Expression of GST isoenzymes was analyzed immunohistochemically. Results Expressions of GSTM1, GSTP1, and GSTT1 were significantly elevated in patients with rosacea compared to those in the control group (P = .0001,P = .0002,P < .0001, respectively). In the rosacea group, GSTT1 expression was significantly stronger than GSTP1 and GSTA1 expressions (P = .019,P < .0001, respectively). There were no significant associations between expressions of GST isoenzymes and gender, age, average duration of illness, disease subtype, ocular involvement, or severity score in the patient group (allP > .05). Conclusions In rosacea, the significant increase of GSTT1, GSTP1, and GSTM1 expressions might result from activation of GST as an outcome of extreme free radical generation from triggered neutrophils or ultraviolet vulnerability. These findings support the relevance of oxidant stress in the pathogenesis of rosacea.
Synthesis, characterization and antibacterial application of silver nanoparticle embedded composite cryogels
(Elsevier, 2020) Erol, Kadir; Bolat Bülter, Melda; Tatar, Demet; Nigiz, Cengiz; Köse, Dursun Ali
Water treatment applications; they consist of processes that desalination, softening, removals of dissolved organic matter, color, particle and microorganism. In traditional treatment processes, microorganisms cause thick film layer on the membrane to be used and not enough treatment. Silver nanoparticles (AgNPs), known to stop bacterial growth due to their superior antibacterial properties even at low concentrations due to their contact with microorganisms, are used for water disinfection. Membrane production with a combination of AgNPs and polymers has started to attract attention in recent years due to advanced optics, conductivity and antibacterial properties. The ability of AgNP to exhibit its antibacterial effect depends on the property of this material to be homogeneously dispersed in the polymer. In this study, Poly(2-hydroxyethyl methacrylate-glycidyl methacrylate), Poly(HEMA-GMA), cryogels were synthesized as solid support. In order to gain antibacterial properties to synthesized cryogels, AgNPs of different sizes were embedded in cryogels. These structures have been used as a kind of antibacterial filter in the purification of water obtained from natural sources used as drinking and using water in the local region. Particle Size analysis was used to determine the size of synthesized AgNPs. Swelling test, Scanning Electron Microscopy (SEM), Elemental Analysis, Fourier Transform Infrared Spectroscopy (FT-IR), and surface area (BET) analysis were performed for characterization of cryogels. (C) 2019 Elsevier B.V. All rights reserved.
Development of novel copper modified cryogels for biochemical isolation
(Springer, 2020) Köse, Kazım; Erol, Kadir; Köse, Dursun Ali
Cu(II) anchored polymeric cryogels are synthesized for the isolation of B12. The poly(2-hydroxyethyl methacrylate) [poly(HEMA)] is used as solid support and N-methacryloyl-l-aspartic acid, is used as a ligand. Synthesis success is proved by the Fourier transform infrared spectroscopy, H-1 and C-13 nuclear magnetic resonance, scanning electron microscope, N-2 adsorption method (Brunauer, Emmet ve Teller), elemental analysis, induction coupled plasma optical emission spectroscopy. In the first part of the work, the polymeric material is synthesized and characterized, and in the second part, adsorptive treatment was carried out to determine the optimum B12 adsorption conditions with this synthesized material in varying conditions. The B12 isolation from cheese is carried out to determine the performance of the polymeric material in the real environment. The antioxidant capacity of the obtained B12 is an indication that the isolation process is successfully carried out.
The expression of GST and CYP isoenzymes in thyroid nodular hyperplasia and papillary thyroid cancer tissue: Correlation with clinical parameters
(Entomology & Applied Science Research Letters-Easletters, 2016) Oğuztüzün, Serpil; Ergin, Duygu; Kılıç, Murat; Moran Bozer, Büşra; Şimşek, Gülçin Güler; Buluş, Hakan
This study investigated the immunohistochemical staining characteristics of glutathione-S-transferase (GST) pi(P), mu(M), theta(T), omega(O) and kappa(K) cytochrome P450 (CYP) A1, B1 and 2E1 isoenzymes in thyroid nodular hyperplasia (NH) and papillary thyroid cancer (PTC) tissues. For immunohistochemical studies, tissues from 18 patients with thyroid nodular hyperplasia, 28 patients with papillary thyroid cancer at the Kecioren Training and Research Hospital, Ankara, Turkey, were used. Relationships between GST and CYP isoenzyme expressions in NH and PTC tissues were examined by the Mann-Whitney U test, and clinicopathological data were examined by the Pearson Correlation Test and Regression Analysis. When the NH and PTC tissues from these cases were compared with respect to their staining intensity, GSTP1, GSTO1, GSTK1, CYP1A1, CYP2E1 expressions in PTC cells were significantly higher than those in NH epithelial cells (p< 0.05). There were no statistically significant differences in the CYP1B1, GSTT1 and GSTM1 expressions between benign and tumor epithelium (p> 0.05). There were significant association between GSTO1, GSTK1 expressions and sT3 levels in PTC (p< 0.05) and CYP1B1 expression in NH. There was a significant association between GSTO1 expression and smoking status in NH ( p< 0.05). There was no statistical relationship between the GSTM1, GSTT1, GSTP1, GSTO1, GSTK1, CYP1A1, CYP1B1, CYP2E1 isoenzyme expressions and the clinicopathological data (age, TSH, sT4 levels, tumor stage) ( p> 0.05). GSTP1, GSTO1, GSTK1, CYP1A1 and CYP2E1 isoenzymes may have roles in the carcinogenesis of the papillary thyroid cancer.
Spectral analysis and biological activity assessment of silver doped hydroxyapatite
(Taylor and Francis Ltd., 2021) Erdem, Ümit; Moran Bozer, Büşra; Türköz, Mustafa B.; Metin, Ayşegül Ülkü; Yıldırım, Gürcan; Türk, Mustafa; Nezir, Saffet
In this study, the hydroxyapatite biomaterials are produced by the precipitation method and the role of silver doping within the different molar ratios of 2.0, 5.0, and 10.0% are investigated with some fundamental analysis, including powder XRD, SEM, EDS, FTIR, Raman, and material densities. In vitro biocompatibility assessment is conducted with cytotoxicity and agar diffusion tests. Moreover, genotoxicity tests determine whether the biomaterials produced cause the mutations or not. In addition, a hemolytic effect test examines the variation of hemolytic behavior of compounds. Also, the cell migration experiments inspect the influence of silver ion levels in biomaterials on many biological processes. The experimental results reveal that the honeycomb-patterned morphological structures are obtained for all the products. FTIR and Raman analyses reveal that the dramatic changes in the characteristic functional group peaks are obtained with the increment in the amount of silver ions. The experimental parts related to the biocompatibility assessment of the study show that there seems to be deterioration in biocompatibility as the silver ion-doping level increases in the system. To sum up, the ideal doping value for bone tissue engineering applications is found to be 2%. © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The Korean Ceramic Society and The Ceramic Society of Japan.
Multi-elemental analysis of flour types and breads by using laser induced breakdown spectroscopy
(Academic Press, 2020) Arı Akın, Pervin; Sezer, Banu; Sanal, Turgay; Apaydın, Hakan; Köksel, Hamit; Boyacı, İsmail Hakkı
Bread and flour are most commonly used products in human diet, which makes it susceptible to adulteration, mislabeling and addition of unpermitted amount of different flour types. The objective of this work was to evaluate the potential of employing laser induced breakdown spectroscopy to differentiate different flour types and quantify the white wheat flour addition to rye and oat flour and breads. In the principal component analysis, score plot represents pure flour types with 97.64% of the variance. In the calibration study, the measured coefficient of determination values was 0.989, 0.989, 0.992 and 0.991 for refined wheat flour: rye flour, refined wheat flour: oat flour, breads made with the blend of refined wheat: rye flour and the blend of refined wheat: oat flour, respectively. The limit of detection values were calculated as 3.82, 5.97, 4.59 and 4.92% for refined wheat flour: rye flour, refined wheat flour: oat flour, refined wheat: rye bread and refined wheat: oat bread, respectively.

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