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Çorum Çevresindeki Yayla Topraklarından İzole Edilen Actinobacteria Üyelerinin Biyosentetik Genlerinin Taranması
(2021) Tatar, Demet; Veyisoğlu, Aysel
Bu çalışmada, Çorum (Kargı ve Osmancık) çevresindeki yaylalardan izole edilen aktinomiset izolatlarının poliketit sentaz ve ribozomal olmayan peptit sentetaz biyosentetik sistemlerinin varlığı moleküler tekniklerle araştırılmıştır. Toplamda 15 tane Actinobacteria üyesinin sekonder metabolit biyosentetik genlerinin (PKS III/NRPS) varlığı PCR temelli incelenmiştir. Yapılan çalışmanın sonuçlarına göre, 15 tane aktinomiset izolatının 7 tanesi PKS-I, 11 tanesi PKS-II ve 14 tanesinin NRPS gen bölgesine sahip olduğu bulunmuştur. Sonuçlar değerlendirildiğinde izolatların nonribozomal peptid sentetaz (NRPS) gen kümelerini tip I-II poliketit sentazlar (PKS-I-II) gen kümelerinden daha fazla içerdiği görülmüştür. Ayrıca 5 izolatın üç gen kümesini bir arada bulundurduğu tespit edilmiştir. Sonuç olarak yaylalardan izole edilen aktinomisetlerin yüksek sekonder metabolit üretme kapasitesine sahip olduğu görülmüştür.
Adli Bilimlerde DNA Parmak İzine Adli Genetik ve Adli Antropolojik Bakış
(2019) Çetli, Elif; Tatar, Demet; Özkoçak, Vahdet
Suç olaylarının çözümlenmesinde ve suçlunun belirlenmesinde yardımcı olan Adli Bilimler alanı gün geçtikçe önemini arttırmaktadır. Olay yerinden elde edilen deliller aracılığı ile kişilerin tanımlanmasında her kişide farklı yapıya sahip olan DNA molekülü olayları çözüme kavuşturmaktadır. Adli amaçlı olarak da kullanılan DNA molekülü Adli Antropoloji ve Adli Genetik alanında kullanılan ortak moleküldür. Antropoloji insan bilimidir ve insanı tüm yönleri ile ele alır. Adli Antropoloji alanı ise suçlunun tespitinde, kimliği belirsiz cesetlerin kimliklendirilmesinde, yaş, cinsiyet ve etnik kökenin belirlenmesinde yardımcı olmaktadır. Antropoloji içerisinde kullanılan Antik DNA sayesinde geçmiş ile günümüz arasında köprü kurulabilmektedir. Olay yerinde her zaman vücut sıvısı veya biyolojik materyaller yer almaz. Bir iskelet kalıntısından olayın çözümlenmesinde devreye Adli Antropoloji girmektedir. İskeletleri kimliklendirme aşamasında Somatoskopi ve Antropometri teknikleri kullanılmaktadır. Bu iki teknik Adli Antropoloji alanında önemli yere sahiptir. Adli Genetik alanı ise olay yerinde bulunan vücut sıvıları ve biyolojik materyaller ile ilgilenir. Bu sıvıları laboratuvar testlerine tabii tutarak DNA analizlerinin sonuçlanmasını sağlamaktadır. Her iki bilim dalının da suçlunun tespitinde DNA molekülünden yararlandığı görülmektedir. DNA molekülünden elde edilen DNA parmak izi kişiye özgüdür ve bu durum suçlu profillerinin belirlenmesi açısından muazzam bir durumdur. DNA Parmak izi ile ilgili yeni gelişmeler söz konusudur. Yeni geliştirilen Optik Koherans Tomografi Sistemi ve ParaDNA Vücut Sıvısı Kimliklendirme Sistemi, DNA parmak izi üzerinde oldukça olumlu sonuçlar ortaya koymaktadır. DNA’nın geri kazanımı ile ilgili çalışmalar sonucunda ise gizli DNA parmak izi olarak tanımlanan izlerin kolayca elde edilebilmesi sağlanmaktadır. Geri kazanım ile olayların çözümlenmesi kısa sürede olmakta ve bu durum birçok laboratuvarın rutin sırasını değiştirmektedir. Aynı zamanda parmak izinin alınması zor olan materyaller üzerinden parmak izinin profillendirilmesine imkân sağlamaktadır.
Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization
(Walter De Gruyter Gmbh, 2021) Erol, Kadir; Tatar, Demet; Veyisoğlu, Aysel; Tokatlı, Ali
Micron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 tg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.
Streptomyces boncukensissp. nov., isolated from saltern soil
(Springer, 2021) Tatar, Demet; Veyisoğlu, Aysel; Saygın, Hayrettin; Şahin, Nevzat
A novel Gram-stain positive, aerobic, non-motile Actinobacterium, designated strain SB3404(T), was isolated from saltern soil collected from Boncuk Saltern, Sungurlu-corum, Turkey, and subjected to a polyphasic taxonomic approach. The organism has shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genusStreptomyces. 16S rRNA gene sequence analysis of strain SB3404(T)showed that it is closely related toStreptomyces albusNBRC 13014(T)(97.2% sequence similarity),Streptomyces xishensisYIM M 10378(T)(96.7%) andStreptomyces abyssalisYIM M 10400(T)(96.5%). The cell wall of the strain containedLL-diaminopimelic acid and the cell-wall sugars were glucose, mannose and ribose. The predominant menaquinones were identified as MK-9(H-8) and MK-9(H-6). The major cellular fatty acids were found to beiso-C-16:0,anteiso-C(17:0)andanteiso-C-15:0. Consequently, strain SB3404(T)is considered to represent a novel species in the genusStreptomyces, for which the nameStreptomyces boncukensissp. nov. is proposed. The type strain is SB3404(T)(= KCTC 49371(T) = JCM 34018(T)).
Diversity and antimicrobial activity of culturable actinobacteria isolated from the sediment of Sarikum Lake
(Taylor & Francis Ltd, 2021) Veyisoğlu, Aysel; Tatar, Demet
In this study, actinobacteria isolated from Sarikum Lake sediment were characterized (Sinop-Turkey). A total of 48 actinobacteria were isolated using ten selective media with the dilution-plating method. The phylogenetic analysis according to 16S rRNA gene sequencing showed that 40% of the isolated strains belong to the widely distributed genus of Streptomyces, 36% belong to genus of Micromonospora, 24% of the isolates belong to rare genera such as Rhodococcus, Plantactinospora, Nonomuraea, Actinomadura and Streptosporangium. Most of the isolated strains belong to the genus Streptomyces (40%) and two isolates may be new species. All of the isolates were tested for antimicrobial activity; only 12 isolates exhibited antimicrobial activity. Nevertheless, 11 isolates were active against gram-positive, 5 were potential against gram-negative and no isolates had any effect against pathogenic fungi. All of the 48 isolates were analysed for genes encoding nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS). In all of the 48 isolates were detected NRPS sequences, PKS-I in 28 isolates and PKS-II in 22 isolates. PKS-I - PKS-II - NRPS genes were identified in 17 isolates.
Isolation, phylogenetic analysis and antimicrobial activity of halophilic actinomycetes from different saline environments located near Çorum province
(Springer, 2021) Tatar, Demet
The aim of this study was to isolate and characterize halophilic actinomycetes. Halophilic actinomycetes isolates were obtained from soil samples from Iskilip-Kekec, Tayrak, Yerlikoy and Sungurlu-Akpinar, Akcakoyunlu, Alibaba, Boncuk salterns located near corum province with a dilution plate technique on four selective media with 3%, 10% and 15% NaCl. PCR amplification belonging to 16S rRNA gene region of the isolates was realized out with primers of 27F and 1525R. Phylogenetic trees were formed with the three different algorithms in MEGA 7.0 software. All fifteen microorganisms were isolated on four selective media with different salt proportions and based on 16S rRNA gene sequence analysis of 15 isolates, eleven Streptomyces isolates, two Nocardiopsis isolates, two Saccharopolyspora isolates were determined. 7 of the strains exhibited only antibacterial activity, 3 only antifungal activity, and 1 both antibacterial and antifungal activities In addition, NRPS, PKS-I ve PKS-II gene regions of isolates with antimicrobial activity were investigated with related primers. According to the results of the work done, of the 11 actinomycete isolates, 4 were found to have PKS-I, 5 were PKS-II and 8 had NRPS gene regions. When the results were evaluated, it was observed that the isolates contained more nonribozomal peptide synthetase (NRPS) gene clusters than type I-II polyketide synthase (PKS-I-II) gene clusters. In addition, it was determined that 1 isolate contain three gene clusters together. As a result, it was found that actinomycetes isolated from saline soils have the capacity to produce secondary metabolites.
Streptomyces coryli sp. nov., isolated from hazelnut orchard soil
(Microbiology Soc, 2020) Saygın, Hayrettin; Veyisoğlu, Aysel; Tatar, Demet; Nigiz, Cengiz; Tokatlı, Ali; Şahin, Nevzat
A novel actinobacteria, isolate A7024(T), was isolated from commercial hazelnut orchard soil sample which was collected at Duzce, West Black Sea region, Turkey. A polyphasic taxonomic study was carried out to determine the status of this isolate. The phylogenetic tree reconstructed using the neighbour-joining algorithm based on 165 rRNA gene sequences indicated that isolate A7024(T) was positioned within the members of the genus Streptomyces with the highest sequence similarity (97.7%) to Streptomyces cadmiisoli ZFG47(T). The organism formed an extensively branched substrate and aerial hyphae which generated irregular rod-shaped spores with smooth-surfaces. The cell wall of strain A7024(T) contained LL-diaminopimelic. Glucose, mannose and ribose were detected as whole-cell sugars. Its polar lipids were diphosphatidylglycerol. phosphatidylethanol-amine, phosphatidylinositol, three unidentified phospholipids and three unidentified glycolipids. Major menaquinones were MK-9(H-6) and MK-9(H-4). The major cellular fatty acids were iso-C-16:0, anteiso-C-1(5:0) and anteiso-C-1(7:)0. Strain A7024(T) had a genome size of 9.0 Mb with a genome G+C content of 71.5 mol%. The low level of 16S rRNA gene similarity, 19.3 +/- 2.3% digital DNA-DNA-hybridization and 76.94% average nucleotide identity values, as well as some different phenotypic characteristics allowed the strain to be distinguished from the closely related type strains. Therefore, it is concluded that strain A7024(T) represents a novel species of the genus of Streptomyces, for which the name Streptomyces coryli sp. nov. is proposed. The type strain is A7024(T) (=DSM 42066(T)=KCTC 29102(T)=NRRL B-24888(T)).
Psikolojik şiddetin örgütsel bağlılığa etkisi
(İSARDER, 2017) Şentürk, Esma Ebru; Yavuz, Ercan
Günümüz yoğun rekabet koşulları, gelişen teknolojiye uyum sağlayabilmek ve hızla artan nüfusun ihtiyaçlarının karşılanabilmesi gibi sebeplerle insanlar her geçen gün çalışma ortamında daha fazla vakit geçirmek zorunda kalmaktadır. Çalışma ortamında geçirilen fazladan saatler karşılıklı çatışmaları arttırmakta ve stresi yükseltmektedir. Yapılan çalışmaların sonuçları ise stresin psikolojik şiddetin temel nedenlerinden biri olduğunu göstermektedir. Sağlık çalışanları çalışma koşullarından ve yaptıkları işin öneminden dolayı stres olgusunu oldukça fazla yaşamaktadırlar. Bu araştırma ile sağlık çalışanlarının psikolojik şiddete maruz kalmalarının örgütsel bağlılıklarına etkisi belirlenmeye çalışılmıştır. Araştırmanın evrenini Ankara ilindeki sağlık örgütlerinde görev alan sağlık çalışanları oluşturmuştur. Tüm evrene ulaşmak mümkün olmadığından basit tesadüfi örneklem yöntemiyle belirlenen 390 kişilik örneklem grubu üzerinden hareket edilmiştir. Araştırmanın problemini desteklemek üzere yapılan analizler sonucunda sağlık çalışanlarının maruz kaldıkları psikolojik şiddet artarken devamlılık bağlılıkları da artmakta ancak duygusal bağlılıkları ve normatif bağlılıkları azalmaktadır
Saccharopolyspora hattusasensis sp. nov., isolated from soil
(Springer Netherlands, 2017) Veyisoğlu, Aysel; Saygın, Hayrettin; Tatar, Demet; İnan Bektaş, Kadriye; Şahin, Nevzat
A Saccharopolyspora strain, designated CR3506 T , isolated from a soil sample collected from Sungurlu, Corum, Turkey, was examinated using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence analysis showed that the strain is closely related to the type strains of Saccharopolyspora spinosa NRRL 18395 T (99.1%), Saccharopolyspora phatthalungensis NRRL B-24798 T (98.4%) and Saccharopolyspora shandongensis 88 T (98.1%); low levels of DNA–DNA relatedness were found between the isolate and S. spinosa and S. phatthalungensis (<50%). Strain CR3506 T was found to have chemotaxonomic and phylogenetic properties consistent with its classification in the genus Saccharopolyspora. The strain contained meso-diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained arabinose and galactose. The polar lipids were identified as phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones (>10%) were MK-9(H 4 ) and MK-8(H 4 ). Major fatty acids were (>10%) iso-C 16:0 , C 15:0 3OH, C 18:0 and iso-C 15:0 . Further, the morphological, physiological and biochemical characteristics of strain CR3506 T are distinct from S. spinosa and other species of the genus Saccharopolyspora with which this strain has high 16S rRNA gene sequence similarity (98.0–98.5%). Strain CR3506 T has antimicrobial activity against Bacillus subtilis NRRL B-209, Citrobacter freundi NRRL B-2643 and Staphylococcus aureus ATCC 29213. Consequently, it is proposed that strain CR3506 T represents a novel Saccharopolyspora species for which the name Saccharopolyspora hattusasensis sp. nov. is proposed. The type strain is CR3506 T (=KCTC 29104 T = DSM 45715 T ). © 2017, Springer International Publishing AG.

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