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  • Küçük Resim
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    Magnetic properties, anticancer and antibacterial effectiveness of sonochemically produced Ce3+/Dy3+ co-activated Mn-Zn nanospinel ferrites
    (Elsevier, 2020) Almessiere, Munirah Abdullah; Slimani, Yassine; Rehman, Sümbül; Khan, Firdos Alam; Dönmez Güngüneş, Çiğdem; Güner, Sadık; Baykal, Abdulhadi
    Some new types of Ce3+ and Dy3+ co-doped manganese-zinc nanospinel ferrites (CDMZNSFs) of the form (Mn0.5Zn0.5)[Fe2-2xCexDyx]O-4 (with 0.0 <= x <= 0.1) were sonochemically produced and characterized. The structure, morphology, optical and magnetic properties of these NSFs were determined as a function of co-dopant (Ce3+ and Dy3+) contents. The direct optical band gap energies of the studied NSFs were ranged from 1.54 to 1.85 eV. The measurements of magnetization versus magnetic field of the prepared NSFs disclosed a superparamagnetic (SPM) behavior at room temperature (RT). The measurements of temperature-dependent magnetizations revealed a transition from superparamagnetic (SPM) state above blocking temperature T-B to a ferromagnetic (FM) state below T-B. The saturation magnetization and T-B decreased with the increase in co-dopant contents. In addition, the bactericidal (on the gram-positive and gram-negative bacterial strains) and anti-cancerous effectiveness of these NSFs were assessed. The cancer cells' growth inhibitory action of these NSFs was tested against both normal (HEK-293) and cancerous (HCT-116) human cells. After 48 h of treatment of the cancerous cells with the NSFs, their population was significantly dropped as shown by the MTT assay, indicating the selective inhibition of the cancer cells growth by the proposed NSFs. Conversely, the non-cancerous cells (HEK-293) population remained unaffected. The IC50 values of the NSFs-treated cancerous cells (HCT-116) were in the range of 0.74-2.35 mu g/mL. The results of the MIC and MBC assays revealed the reasonable antibacterial efficacy (growth inhibitory activity) of these NSFs when tested against the E. coli and S. aureus bacterial strains. It is established that the proposed Ce3+/Dy3+ co-activated CDMZNSFs may be beneficial for the anti-cancerous and bactericidal applications. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
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    Öğe
    Exploration of catalytic and cytotoxicity activities of CaxMgxNi1-2xFe2O4 nanoparticles
    (Elsevier Science Sa, 2019) Nawaz, Muhammad; Almessiere, Munirah Abdullah; Almofty, Sarah; Dönmez Güngüneş, Çiğdem; Slimani, Yassine; Baykal, Abdulhadi
    The CaxMgxNi1-2xFe2O4 (x <= 0.05) nanoparticles (NPs) have been synthesized hydrothermally. The XRD analyses confirmed the purity of all products and their spherical morphology and compositions were explained by SEM, TEM, EDX and Elemental mapping analyses. Results confirmed the composition of CaxMgxNi1-2xFe2O4. The surface area of them was determined by BET analysis. Results indicated that when x is increased surface area of CaxMgxNi1-2xFe2O4 is decreased as compare with x = 0.0, however, at high composition (x = 0.05), surface area is increased. Catalytic activity of CaxMgxNi1-2xFe2O4 (x <= 0.05) NPs was studied for the reduction of 4-nitrophenol as a model compound. Reaction was monitored by UV-spectrophotometer at room temperature and at different times. Among different compositions of CaxMgxNi1-2xFe2O4, it was noticed that x = 0.04 exhibited more activity for the reduction of 4-nitrophenol and x = 0.03 was observed least active. Results suggested that CaxMgxNi1-2xFe2O4 could be useful material for catalytic applications. Cytotoxicity activity of CaxMgxNi1-2xFe2O4 (x <= 0.05) NPs against HCT116 (Human colon cancer cell line) cell line and MCF-7 (breast cancer cell line) was evaluated by using MTT assay. It was observed that CaxMgxNi1-2xFe2O4 (x <= 0.05) NPs exhibited less cytotoxicity. Due to less cytotoxicity, CaxMgxNi1-2xFe2O4 may also be useful in the field of biomedical applications.
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    Öğe
    The Effect of Folic Acid- and Caffeic Acid-Functionalized SPION on Different Cancer Cell Lines
    (Springer New York LLC, 2018) Dönmez Güngüneş, Çiğdem; Alpsoy, Lokman; Baykal, Abdulhadi; Nawaz, Muhammad; Ülker Akal, Zeynep
    In this study, caffeic acid (CFA)- and folic acid (FA)-conjugated superparamagnetic iron oxide bio-nanocomposite (SPION@PEG-FA-@CFA) was prepared and its cytotoxic and apoptotic effects against murine fibroblast (L929), glioblastoma (U87), adenocarcinoma, human breast cancer (MCF-7), epithelioid cervix carcinoma (HeLa), and lung carcinoma (A549) have been determined. A real-time cell analyzer system (RTCA) for cytotoxicity, annexin assay and TUNEL, caspase 3/7 expression level, and caspase 3/7 activities for apoptosis were used. The RTCA showed that the cytotoxic effects of the bio-nanocomposite were higher in U87, MCF-7, and HeLa cells (which are folic acid-positive cells (FAR (+))). TUNEL, annexin, and caspase assay results indicated that the bio-nanocomposite caused apoptotic and necrotic impacts on cells. Necrotic and apoptotic cell ratios in FAR-positive cells (L929 and A549) were lesser than those in U87, MCF-7, and HeLa cells (FAR (+)). Therefore, the obtained results showed that the bio-nanocomposite can be used as a nano-medicine for biomedical application (cancer treatments). Moreover, this work can also be useful for designing other functionalized materials which could act as biomaterial. This study will be also informative for the researchers to design novel materials for biomedical applications. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
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    Öğe
    A comparative study on the in vitro cytotoxic responses of two mammalian cell types to fullerenes, carbon nanotubes and iron oxide nanoparticles
    (Taylor and Francis Ltd, 2017) Dönmez Güngüneş, Çiğdem; Şeker, Şükran; Eser Elçin, Ayşe; Elçin, Yaşar Murat
    The present study was designed to evaluate and compare the time- and dose-dependent cellular response of human periodontal ligament fibroblasts (hPDLFs), and mouse dermal fibroblasts (mDFs) to three different types of nanoparticles (NPs); fullerenes (C60), single walled carbon nanotubes (SWCNTs) and iron (II,III) oxide (Fe3O4) nanoparticles via in vitro toxicity methods, and impedance based biosensor system. NPs were characterized according to their morphology, structure, surface area, particle size distribution and zeta potential by using transmission electron microscopy, X-ray diffraction, Brunauer–Emmett–Teller, dynamic light scattering and zeta sizer analyses. The Mössbauer spectroscopy was used in order to magnetically characterize the Fe3O4 NPs. The hPDLFs and mDFs were exposed to different concentrations of the NPs (0.1, 1, 10, 50 and 100 ?g g/mL) for predetermined time intervals (6, 24 and 48 h) under controlled conditions. Subsequently, NP exposed cells were tested for viability, membrane leakage and generation of intracellular reactive oxygen species. Additional to in vitro cytotoxicity assays, the cellular responses to selected NPs were determined in real time using an impedance based biosensor system. Taken together, information obtained from all experiments suggests that toxicity of the selected NPs is cell type, concentration and time dependent. © 2016 Informa UK Limited, trading as Taylor & Francis Group.