Taner, GökçeÖzkan Vardar, DenizAydın, SevtapAytaç, ZekiBaşaran, AhmetBaşaran, Nurşen2019-05-102019-05-102017Taner, G., Özkan Vardar, D., Aydin, S., Aytaç, Z., Başaran, A., & Başaran, N. (2017). Use of in vitro assays to assess the potential cytotoxic, genotoxic and antigenotoxic effects of vanillic and cinnamic acid. Drug and chemical toxicology, 40(2), 183-190.0148-0545https://doi.org/10.1080/01480545.2016.1190740https://hdl.handle.net/11491/725Vanillic acid (VA) found in vanilla and cinnamic acid (CA) the precursor of flavonoids and found in cinnamon oil, are natural plant phenolic acids which are secondary aromatic plant products suggested to possess many physiological and pharmacological functions. In vitro and in vivo experiments have shown that phenolic acids exhibit powerful effects on biological responses by scavenging free radicals and eliciting antioxidant capacity. In the present study, we investigated the antioxidant capacity of VA and CA by the trolox equivalent antioxidant capacity (TEAC) assay, cytotoxicity by neutral red uptake (NRU) assay in Chinese Hamster Ovary (CHO) cells and also the genotoxic and antigenotoxic effects of these phenolic acids using the cytokinesis-blocked micronucleus (CBMN) and the alkaline comet assays in human peripheral blood lymphocytes. At all tested concentrations, VA (0.17–67.2 ?g/ml) showed antioxidant activity but CA (0.15–59.2 ?g/ml) did not show antioxidant activity against 2,2-azino-bis (3-ethylbenz-thiazoline-6-sulphonic acid) (ABTS). VA (0.84, 4.2, 8.4, 16.8, 84 and 168 ?g/ml) and CA (0.74, 3.7, 7.4, 14.8, 74, 148 ?g/ml) did not have cytotoxic and genotoxic effects alone at the studied concentrations as compared with the controls. Both VA and CA seem to decrease DNA damage induced by H2O2 in human lymphocytes. © 2016 Informa UK Limited, trading as Taylor & Francis Group.eninfo:eu-repo/semantics/closedAccessCHO CellsCinnamic AcidComet AssayCytokinesis-Block Micronucleus AssayHuman LymphocytesNeutral Red Uptake AssayTrolox Equivalent Antioxidant CapacityVanillic AcidArticle40218319010.1080/01480545.2016.1190740N/AQ1