Bakhshpour, MonirehTamahkar Irmak, EmelAndaç, MügeDenizli, Adil2019-05-132019-05-132017Bakhshpour, M., Tamahkar, E., Andaç, M., Denizli, A. (2017). Affinity binding of proteins to the modified bacterial cellulose nanofibers. Journal of Chromatography B, 1052, 121-127.1570-0232https://doi.org/10.1016/j.jchromb.2017.03.021https://hdl.handle.net/11491/1647The potential of the modified bacterial cellulose (BC) nanofibers was determined bearing metal ion coordination interactions to enhance the protein adsorption and binding capacity. Thus, a household synthesized metal chelating monomer, namely N-methacryloyl-L-histidine methylester (MAH), and a commercial metal chelating monomer, namely 4-vinylimidazole (VIm), were used to complex with metal ions Cu(II) and Ni(II) respectively for the synthesis of the modified BC nanofibers. The modified nanofibers were characterized by FT-IR, SEM and EDX measurements. The protein adsorption tests were carried out using hemoglobin as a model protein and it was determined that the maximum adsorption capacity of hemoglobin onto the modified BC nanofibers was found as 47.40 mg/g. The novel strategy for the preparation of metal chelated nanofibers was developed. © 2017 Elsevier B.V.eninfo:eu-repo/semantics/closedAccessBacterial Cellulose NanofibersMetal Ion CoordinationProtein RecognitionArticle105212112710.1016/j.jchromb.2017.03.021N/AQ2