Yılmaz, YATosun, MIDemirkan, ECan, SÖzkan, AArıcı, MKutlu, MAyrancı, MMarkovic, MArıcı, İEGüneş, MOKowalski, T2026-03-312026-03-3120252045-2322http://dx.doi.org/10.1038/s41598-025-14797-0https://hdl.handle.net/11491/9670This study sought to establish an optimal inspiratory warm-up (IWU) protocol for elite 400-meter athletes. It addresses the existing gap in sprint-specific research and provides practical applications for coaches and athletes aiming to optimize sprint running performance. Across four weeks, 13 elite male runners (age: 22.7 +/- 2, personal best: 50.78 +/- 0.65 s) performed four 400-meter time trials after different warm-up protocols in a randomized order. Assessments of various respiratory variables [maximal inspiratory pressure (MIP), maximal expiratory pressure (MEP), peak inspiratory flow rate (PIFR), inhaled volume (IV)] and selected physiological indices [heart rate (HR) and blood lactate (bLa)] were performed before and after the time trials. Analysis of variance with a significance level of p < 0.05 was applied. Incorporating IWU into a standard running warm-up resulted in a similar to 0.38-second faster 400-meter time trial in elite male sprinters (p < 0.001). The most effective IWU protocols, associated with the fastest run splits, consist of two sets of 30 dynamic inspiratory maneuvers performed with pressure threshold loading at 40% or 60% of MIP resistance. These protocols exhibited a positive influence on selected respiratory indices and significantly lowered post-run lactate concentrations compared to the athletic warm-up and sham protocol. IWU with 40% MIP resistance was associated with lower post-run HR compared to other protocols. IWU using pressure threshold loading at either 40% or 60% of MIP can be beneficial for elite male sprinters. While 40% MIP appears to support faster recovery, a higher intensity at 60% MIP shows greater effectiveness in enhancing 400-meter sprint performance. Future research should explore IWU applications across broader populations and conditions, including female athletes, various hypoxic environments, and temperature variations.enArticle15110.1038/s41598-025-14797-0WOS:00154687430003740775048