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    Effect of inulin and auricularia polytricha extract on proliferation of lactobacillus rhamnosus
    (Hitit University, 2017) Alp Avcı, Gülçin; Özçelik, Burçin; Kesepara, Elmas Ceren
    Probiotic microorganisms have many health-beneficial effects on gastrointestinal system and their therapeutic usage is becoming increasingly common in human and veterinary medicine. Many different species and strains of bacteria, yeast and even fungi have been extensively used as potential probiotics. Among the probiotic strains, Lactobacillus rhamnosus is one of the most commonly used strain for probiotic treatment and health promoting functions of this strain are well documented. To enhance therapeutic effects of probiotics, prebiotics have been extensively used. Prebiotics stimulate the proliferation of probiotics and this may have positive effects on the maintenance of the balance between pathogenic and nonpathogenic bacteria. In this study, we aimed to evaluate the effect of inulin and Auricularia polytricha aqueous extract on the proliferation of L. rhamnosus. For this purpose, L. rhamnosus was inoculated in three different MRS broth supplemented with inulin 5%, A. polytricha extract 5% and with the mixture of inulin 5% plus A. polytricha extract 5%. Our results indicated that L. rhamnosus was able to use inulin and fungus extract as a carbon source. Moreover,combined use of inulin and A. polytricha improved prebiotic efficacy.
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    Öğe
    Production of an alkaline protease using Bacillus pumilus D3 without inactivation by SDS, its characterization and purification
    (Informa Healthcare, 2014) Özçelik, Burçin; Aytar, Pınar; Gedikli, Serap; Yardımcı, Ezgi; Çalışkan, Figen; Çabuk, Ahmet
    In this study, protease-producing capacity of Bacillus pumilus D3, isolated from hydrocarbon contaminated soil, was evaluated and optimized. Optimum growing conditions for B. pumilus D3 in terms of protease production were determined as 1% optimum inoculum size, 35 °C temperature, 11 pH and 48 h incubation time, respectively. Stability studies indicated that the mentioned protease was stable within the pH range of 7-10.5 and between 30 °C and 40 °C temperatures. Surprisingly, the activity of the enzyme increased in the presence of SDS with concentration up to 5 mM. The protease was concentrated 1.6-fold with ammonium sulfate precipitation and dialysis. At least six protein bands were obtained from dialysate by electrophoresis. Four clear protein bands with caseinolytic activity were detected by zymography. Dialysate was further purified by anion-exchange chromatography and the caseinolytic active fraction showed a single band between 29 and 36 kDa of reducing conditions. © 2014 Informa UK Ltd.