Production of an alkaline protease using Bacillus pumilus D3 without inactivation by SDS, its characterization and purification
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Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Informa Healthcare
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, protease-producing capacity of Bacillus pumilus D3, isolated from hydrocarbon contaminated soil, was evaluated and optimized. Optimum growing conditions for B. pumilus D3 in terms of protease production were determined as 1% optimum inoculum size, 35 °C temperature, 11 pH and 48 h incubation time, respectively. Stability studies indicated that the mentioned protease was stable within the pH range of 7-10.5 and between 30 °C and 40 °C temperatures. Surprisingly, the activity of the enzyme increased in the presence of SDS with concentration up to 5 mM. The protease was concentrated 1.6-fold with ammonium sulfate precipitation and dialysis. At least six protein bands were obtained from dialysate by electrophoresis. Four clear protein bands with caseinolytic activity were detected by zymography. Dialysate was further purified by anion-exchange chromatography and the caseinolytic active fraction showed a single band between 29 and 36 kDa of reducing conditions. © 2014 Informa UK Ltd.
Açıklama
Anahtar Kelimeler
Bacillus Pumilus, Hydrocarbon-Contaminated Soil, Production, Protease, Purification
Kaynak
Journal of Enzyme Inhibition and Medicinal Chemistry
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
29
Sayı
3
Künye
Özçelik, B., Aytar, P., Gedikli, S., Yardımcı, E., Çalışkan, F., Çabuk, A. (2014). Production of an alkaline protease using Bacillus pumilus D3 without inactivation by SDS, its characterization and purification. Journal of Enzyme Inhibition and Medicinal Chemistry, 29(3), 388-396.
