Yazar "Mesci, Seda" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • Küçük Resim
    Öğe
    A multidisciplinary approach to coronavirus disease (COVID-19)
    (MDPI, 2021) Gediz Ertürk, Aliye; Şahin, Arzu; Batı Ay, Ebru; Pelit, Emel; Bağdatlı, Emine; Kulu, İrem; Gül, Melek; Mesci, Seda; Eryılmaz, Serpil; Oba İlter, Şirin; Yıldırım, Tuba
    Since December 2019, humanity has faced an important global threat. Many studies have been published on the origin, structure, and mechanism of action of the SARS-CoV-2 virus and the treatment of its disease. The priority of scientists all over the world has been to direct their time to research this subject. In this review, we highlight chemical studies and therapeutic approaches to overcome COVID-19 with seven different sections. These sections are the structure and mechanism of action of SARS-CoV-2, immunotherapy and vaccine, computer-aided drug design, repurposing therapeutics for COVID-19, synthesis of new molecular structures against COVID-19, food safety/security and functional food components, and potential natural products against COVID-19. In this work, we aimed to screen all the newly synthesized compounds, repurposing chemicals covering antiviral, anti-inflammatory, antibacterial, antiparasitic, anticancer, antipsychotic, and antihistamine compounds against COVID-19. We also highlight computer-aided approaches to develop an anti-COVID-19 molecule. We explain that some phytochemicals and dietary supplements have been identified as antiviral bioproducts, which have almost been successfully tested against COVID-19. In addition, we present immunotherapy types, targets, immunotherapy and inflammation/mutations of the virus, immune response, and vaccine issues.
  • Küçük Resim
    Öğe
    Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTXand IntI in Klebsiella pneumoniae
    (2021) Güçkan, Rıdvan; Kılınç, Çetin; Başkan, Ceren; Yazgan, Burak; Mesci, Seda; Yıldırım, Tuba
    Aim: Klebsiella pneumoniae as an opportunistic pathogen is responsible for nosocomial, urinary tract infections, pneumonia, bacteremia, liver abscesses, and respiratory and blood infections in patients. The aim of our study is to examine the antibiotic sensitivity, expression levels of IntI, TEM, SHV and CTX genes, which play a role in multidrug resistance, biofilm formation and even the relationships among them. Materials and Methods: Identification was implemented by carrying out the VITEK2 system and phenotypic confirmation of the ESBL producing isolates by using a combined disc test. Antibiotic sensitivity was implemented by disc diffusion method and microdilution methods toward various antibiotics. Furthermore, biofilm formation was examined through microtitration plate method. Results: The expressions of IntI, TEM, SHV and CTX were implemented in qRT-PCR. 19 were susceptible to all antibiotics, 17 were ESBL (+) and 16 were carbapenem-resistant among 52. The expression of these genes was upregulated in ESBL (+) strains and produced biofilm and the expression of these genes was upregulated, too. Conclusion: The results of the study revealed the potential role of the mentioned genes in biofilm formation and antibiotic resistance, enabling the development of new drugs. Further studies will be essential so as to determine when or how these systems are included in antibiotic resistance and biofilm formation.
  • Küçük Resim
    Öğe
    Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines
    (Marmara Univ, Inst Health Sciences, 2021) Mesci, Seda; Gül, Melek; Yıldırım, Tuba
    Objective: To investigate the antioxidant and cytotoxic activities of sulfur-containing glycine imine derivatives MCF-7 (human breast adenocarcinoma) and DLD-1 (colorectal adenocarcinoma) cell lines. Methods: This study examined the antioxidant activities (25-200 mu M) of sulfur-containing glycine imine derivatives via the DPPH, metal chelating and reduction methods. Furthermore the cytotoxic activity of MCF-7, MCF-12A (normal breast epithelial) and DLD-1, CCD-18CO (normal colon fibroblast) were examined with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and RTCA (Real-time Cell Analysis) assays. Results: The antioxidant assay of the metal chelating activity showed significant results (71, 77 and 40% respectively) as compared to knowing synthetic antioxidant (trolox; 95.45, EDTA; 97.06 %). Reducing activity was found to be very low compared to the standard compounds. Compounds were shown to be moderated by DPPH (2,2-Diphenyl-1-picrylhydrazyl) activity, and the IC50 value ranged from 91 to 150. The IC50 values (100 mu M) of the MTT and RTCA analyses were similar. Conclusion: The study showed that the compounds had selective and significant antioxidant activities, and we also found that they had cytotoxic effects on MCF-7 and DLD-1 cells.
  • Küçük Resim
    Öğe
    Aquatic coleoptera fauna of Çorum and Yozgat provinces (Turkey)
    (Ersin Yücel, 2010) Darılmaz, Mustafa Cemal; Salur, Ali; Mesci, Seda
    Aquatic beetles in the families Gyrinidae, Haliplidae, Noteridae, Dytiscidae, Helophoridae and Hydrophilidae were sampled at the Çorum and Yozgat provinces in Central Anatolian from 2006 through 2009. 61 species of water beetles were recorded in Çorum province and 15 species in Yozgat province. 56 species appeared to be new for Çorum and 14 for Yozgat. Of these beetles 13 species are here recorded for the first time from the Central Anatolian region. The known ranges of these species are expanded by the new findings
  • [ X ]
    Öğe
    Downregulation of HSP27 by isoindole-derived pyrrolidines suppressing multidrug resistance (MDR) and inducing apoptosis in MCF-7 and DLD-1 cell lines
    (NATL INST SCIENCE COMMUNICATION-NISCAIR, 2024) Mesci, Seda; Yazgan, Burak; Gül, Melek; Yıldırım, Tuba
    In most cancer treatments, major problem arises from the prevention of cell death (apoptosis suppression) with the development of drug resistance. Anticancer agents that ensure elimination of drug resistance and drug-resistant cells to apoptosis, are among the main targets. Here, we evaluated a series of synthesized N-phenyl maleimide substituents in tetracyclic compounds as anticancer drug candidate. We selected compounds may lead to death and eliminate drug resistance in breast and colon cells. In MCF-7 and DLD-1 cell lines; multidrug resistance genes (ABCB1, ABCC3, ABCC10, ABCC11 and ABCG2), apoptosis mechanism genes (BAX, BCL-2, p53, PARP and CASP3), heat shock genes (HSP27, HSP40, HSP60, HSP70 and HSP90?) and endoplasmic reticulum (ER) chaperone genes (GRP78 and GRP94) mRNA levels were determined by qPCR method. Amounts of proteins of apoptosis and signalling pathways were measured by human apoptosis antibody array. The compounds have been shown to have downregulation on multidrug resistance genes other than ABCC3. It was found that all compounds in MCF-7 and DLD-1 cells showed significant increase in p53, BAX and CASP3 gene expressions. Also, the compounds have the potential to reduce gene expression of heat shock genes (HSPs). While the compounds have been determined to increase protein expression in BAD, BAX, BID, BIM, Caspase-3, Caspase-7, Caspase-8, Cytochrome-C, Fas, TNF, TRAIL, p27, p38 and p53; decrease protein expression in AKT, BCL-2, ERK1/2, HSP27, HSP60, IGFs, JNK, NFKB, PARP, TAK1, Survivin in MCF-7 and DLD-1 cells. The compounds stand out with their inhibition of HSP27 in DLD-1 cells and their inhibition with HSP27 and NFkB in MCF-7 cells. Overall, it has been shown that these compounds increase intrinsic and extrinsic proapoptotic proteins, decrease antiapoptotic proteins, decrease HSPs and some growth factors, and they may serve as potential anticarcinogenic molecules.
  • Küçük Resim
    Öğe
    Investigation of antioxidant activity and effect on cell viability of pyrrolidine compounds in MCF-7 (breast cancer) and DLD-1 (colon cancer) cell lines
    (Al-Farabi Kazakh Natl Univ, 2021) Mesci, Seda; Gül, Melek; Yıldırım, Tuba
    In this study, it is aimed to investigate the effects of pyrrolidine compounds that are thought to be new drug candidates with effect on cell viability on cancer (MCF-7 and DLD-1) and normal (MCF-12A and CCD-18CO) cell lines. The effect on cell viability of the pyrrolidine compounds was determined for 24 hours at different concentrations (25-100 mu M) on cancer (MCF-7 and DLD-1) and normal (MCF-12A and CCD-18CO) cell lines by comparing MIT and RTCA assays. The antioxidant activities of the pyrrolidine compounds were determined at different concentrations (25-200 mM) with DPPH, metal chelating and reducing methods. In addition, the calculation of the structural properties of the new pyrrolidine scaffolding with the DFT method was presented. It has been determined that pyrrolidine compounds reduce the number of MCF-7 cancer cells according to a negative control by half of and inhibit the DLD-1 cancer cells with the MTT method. IC50 value for MCF-7 cell was determined as 100 mu M. Metal chelation and DPPH activities have close results. The IC50 value of DPPH activity of the compounds ranged from 108 to 142 mu g/mL.