Neuroprotective effects of hesperidin on cerebral vasospasm after subarachnoid hemorrhage in rats: an experimental study

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dc.contributor.authorKeskin, E
dc.contributor.authorYılmaz, B
dc.contributor.authorGel, MS
dc.date.accessioned2026-03-31T13:21:19Z
dc.date.available2026-03-31T13:21:19Z
dc.date.issued2025
dc.description.abstractBACKGROUND: Subarachnoid hemorrhage (SAH) is a severe neurological emergency with high morbidity and mortality, primarily due to vasospasm and delayed ischemia. Hesperidin (HSP), a natural flavonoid, possesses strong antioxidant and vasoprotective properties. This experimental animal study examined HSP's neuroprotective role in oxidative stress and vascular remodeling after SAH, focusing on the extracellular regulated kinase 5-Kruppel-like factor 2-endothelial nitric oxide synthase (Erk5-KLF2-eNOS) pathway. METHODS: The study was conducted from 2021 to 2022. Forty female Wistar albino rats were divided into five groups: control (G1, n=8), sham (G2, n=8), SAH + Vehicle (G3, n=8), SAH with low-dose HSP (G4, n=8), and SAH with high-dose HSP (G5, n=8). SAH was induced using a double injection of homologous blood into the cisterna magna. Biochemical markers (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPx], nitric oxide [NOS]), basilar artery morphometry, and molecular expressions (Erk5, p-Erk5, KLF2, eNOS) were evaluated 48 hours post-SAH. RESULTS: SAH significantly increased oxidative stress and reduced vascular lumen diameter in untreated rats (G3). Both HSP-treated groups (G4 and G5) showed improved antioxidant enzyme levels (SOD, CAT, GPx) and near-normal NOS levels. Morphometric analysis demonstrated significant preservation of basilar artery lumen diameter in treated groups, with no significant changes in wall thickness. Molecular analysis revealed upregulation of the Erk5-KLF2-eNOS pathway, suggesting a role in vasodilation and mitigation of oxidative stress. CONCLUSION: HSP protects against SAH-induced vasospasm and oxidative damage by enhancing antioxidant capacity and modulating the Erk5-KLF2-eNOS pathway, suggesting its therapeutic potential.
dc.identifier.doi10.14744/tjtes.2025.52931
dc.identifier.issn1306-696X
dc.identifier.issn1307-7945
dc.identifier.issue12
dc.identifier.pmid41424426
dc.identifier.urihttp://dx.doi.org/10.14744/tjtes.2025.52931
dc.identifier.urihttps://hdl.handle.net/11491/9667
dc.identifier.volume31
dc.identifier.wosWOS:001653325800001
dc.language.isoen
dc.publisherTURKISH ASSOC TRAUMA EMERGENCY SURGERY
dc.relation.ispartofULUS TRAVMA ACIL CER
dc.subjectHesperidin
dc.subjectsubarachnoid hemorrhage
dc.subjectrat
dc.subjectvasospasm
dc.titleNeuroprotective effects of hesperidin on cerebral vasospasm after subarachnoid hemorrhage in rats: an experimental study
dc.typeArticle

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