Experimental modeling and optimization of pullulan production by Aureobasidium pullulans AZ?6

dc.authorid0000-0002-8741-0410
dc.contributor.authorMüjdeci, Gamze Nur
dc.contributor.authorBozdemir, Melek Tijen
dc.contributor.authorÖzbaş, Zekiye Yeşim
dc.date.accessioned2024-08-07T07:05:14Z
dc.date.available2024-08-07T07:05:14Z
dc.date.issued2024en_US
dc.departmentHitit Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü
dc.description.abstractAbstract The objective of this study was to optimize the fermentation parameters by using response surface methodology for increasing pullulan production of Aureobasidium pullulans AZ-6 in synthetic fermentation medium. The optimum conditions were determined as follows: the initial sucrose concentration (X1): 100 g/L; the initial peptone concentration (X2): 11.31 g/L; the initial pH (X3): 6.48; and the temperature (X4): 24.2 °C. In the optimum conditions, the maximum exopolysaccharide (EPS) concentration (Y1), the maximum pullulan concentration (Y2), the specifc growth rate (Y3), the maximum specifc pullulan formation rate (Y4), and the pullulan yield (Y5) were expected to be obtained as 37.078 and 35.372 g/L, 0.062 h?1, 0.021 [g pullulan/(g mo.h)], and 53.681%, respectively. The observed values of Y1, Y2, Y3, Y4, and Y5 were 36.950 g/L, 35.470 g/L, 0.064 h?1, 0.036 [g pullulan/(g mo.sa)], and 54.480%, respectively, as a result of validation experiments. EPS samples were characterized by Fourier-transform infrared spectroscopy and scanning electron microscope analyses. The optimization process caused more than a 50% increase in EPS and pullulan concentrations. This study showed that the color-variant A. pullulans AZ-6 strain could become a signifcant industrial strain if it is explored further in the future to produce pullulan on a larger scale.
dc.description.provenanceSubmitted by Zeynep Umut NARİN (umutarslan@hitit.edu.tr) on 2024-08-07T07:04:06Z No. of bitstreams: 1 gamzenur-mujdeci2024.pdf: 6462469 bytes, checksum: b21ac6e0eb5dfdf9026ec8c3bb49ced7 (MD5)en
dc.description.provenanceApproved for entry into archive by Zeynep Umut NARİN (umutarslan@hitit.edu.tr) on 2024-08-07T07:05:14Z (GMT) No. of bitstreams: 1 gamzenur-mujdeci2024.pdf: 6462469 bytes, checksum: b21ac6e0eb5dfdf9026ec8c3bb49ced7 (MD5)en
dc.description.provenanceMade available in DSpace on 2024-08-07T07:05:14Z (GMT). No. of bitstreams: 1 gamzenur-mujdeci2024.pdf: 6462469 bytes, checksum: b21ac6e0eb5dfdf9026ec8c3bb49ced7 (MD5) Previous issue date: 2024en
dc.identifier.citationMujdeci, G. N., Bozdemir, M. T., & Ozbas, Z. Y. (2024). Experimental modeling and optimization of pullulan production by Aureobasidium pullulans AZ-6. Polymer Bulletin, 1-42.
dc.identifier.doi10.1007/s00289-024-05146-8
dc.identifier.urihttps://doi.org/10.1007/s00289-024-05146-8
dc.identifier.urihttps://hdl.handle.net/11491/9050
dc.identifier.wosWOS:001156026600002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.institutionauthorMüjdeci, Gamze Nur
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofPOLYMER BULLETIN
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAureobasidium pullulansen_US
dc.subjectPullulanen_US
dc.subjectOptimizationen_US
dc.subjectResponse surface methodologyen_US
dc.subjectCentral composite rotatable designen_US
dc.titleExperimental modeling and optimization of pullulan production by Aureobasidium pullulans AZ?6
dc.typeArticle

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