Effects of mesenchymal stem cell and amnion membrane transfer on prevention of pericardial adhesions

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dc.contributor.authorKabalci, Mehmet
dc.contributor.authorSahin, Mustafa
dc.contributor.authorPekcan, Zeynep
dc.contributor.authorZengin, Mehmet
dc.contributor.authorDogru, Mehmet Tolga
dc.contributor.authorKisa, Ucler
dc.date.accessioned2021-11-01T15:05:04Z
dc.date.available2021-11-01T15:05:04Z
dc.date.issued2020
dc.department[Belirlenecek]
dc.description.abstractBackground: To investigate and compare the antiadhesive/antifibrotic effects of mesenchymal stem cells (MSC) and amnion membrane transfer (AMT) in a rat model. Material and methods: Three experimental and sham groups were formed using 30 Wistar-Albino rats. AMT and MSC were applied to the related groups. The control group was not treated. After 12 weeks follow-up, intracardiac blood and cardiac-pericardiac tissue samples were taken. The severity of adhesions and fibrosis were scored macroscopically and microscopically with Hematoxylin/ Eosin and Masson's trichrome staining. TNF-alpha, TGF-beta, IL-1, PDGF, FGF, VEGF and Caspase-3 levels were measured with the ELISA method. Results: Severe adhesions were observed in the AMT and control groups, but no adhesion was present in the MSC group. Pericardial thickness, increased vascularity, fibrosis, and collagen accumulation were similar between control and AMT groups, but were less in Sham and MSC groups. Between MSC and AMT groups, only Caspase-3 level was different, which is an apoptosis marker. Conclusion: The positive effects of MSC on adhesion, which we achieved in our study, suggest that it may prevent adhesion. AMT did not provide a positive effect. The correlation of Caspase-3 with postoperative adhesion/fibrosis should be examined in more detail.
dc.identifier.doi10.1515/tjb-2019-0309
dc.identifier.endpage414en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue4en_US
dc.identifier.startpage405en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2019-0309
dc.identifier.urihttps://hdl.handle.net/11491/7090
dc.identifier.volume45en_US
dc.identifier.wosWOS:000582567600009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.institutionauthor[Belirlenecek]
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofTurkish Journal Of Biochemistry-Turk Biyokimya Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMesenchymal stem cellen_US
dc.subjectAmnion membrane transferen_US
dc.subjectPericardial adhesionen_US
dc.subjectRaten_US
dc.subjectInflammatory markersen_US
dc.titleEffects of mesenchymal stem cell and amnion membrane transfer on prevention of pericardial adhesions
dc.typeArticle

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