Aminoasit bağlı kriyojellerin sentezi ve biyolojik uygulamaları
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Dosyalar
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hitit Üniversitesi Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Kriyojeller son yıllarda oldukça fazla tercih edilen sentetik materyallerdir. Bu yapılar sürekli ve kesikli sistemde adsorban olarak kullanılmaktadır. Bu çalışmada poli(2- Hidroksietilmetakrilat-N-metakriloil-L-lizin), poli(HEMA-MAL), kriyojeller sentezlenmiş ve L-aspartik asidin sulu sistemlerden adsorpsiyonunda kullanılmıştır. Kriyojellerin aspartik asit adsorpsiyonu değişen pH, etkileşim süresi, başlangıç aspartik asit derişimi, sıcaklık ve iyonik şiddet koşullarında test edilmiştir. Kriyojellerin karakterizasyonu için; şişme testi, Fourier Transform Infrared spektroskopisi (FT-IR) analizi, taramalı elektron mikroskobu (SEM) analizi, termal analiz, yüzey alanı analizi ve elementel analiz yapılmıştır. Kriyojellerin adsorpsiyon kapasitesi yaklaşık olarak 236,0 mg aspartik asit/g kriyojel olarak hesaplanmıştır. Belirlenen optimum adsorpsiyon koşulları ile lizozim enziminin adsorpsiyonu çalışılmış ve adsorpsiyon kapasitesi 37,25 mg enzim/g kriyojel olarak tespit edilmiştir. Adsorpsiyon işleminin adsorpsiyon modeli Langmuir ve Freundlich adsorpsiyon modelleri üzerinden analiz edilmiş ve Langmuir adsorpsiyon modelinin daha uygun olduğu belirlenmiştir.
Cryogel quite preferred as synthetic materials in recent years has emerged. Adsorbents are used in the batch and continuous system operation. In this study poly(2- Hydroxyethyl methacrylate-N-methacryloyl-L-lysine), poly(HEMA-MAL), cryogels synthesized and used in adsorption from aqueous systems of L-aspartic acid. The adsorption of aspartic acid with the cryogels was tested under changing conditions of pH, interaction time, initial aspartic acid concentration, temperature, and ionic strength. For the characterization of cryogels; swelling test, Fourier Transform Infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), thermal analysis, surface area (BET), elemental analysis were performed. The adsorption capacity of cryogels was determined about 236,0 mg aspartic acid/g cryogel. Adsorption of lysozyme was studied under the optimum adsorption conditions and adsorption capacity of cryogels was found as 37,25 mg enzyme/g cryogel. The adsorption process was triad via Langmuir and Freundlich isotherm models and the Langmuir adsorption model was determined to be more appropriate for the aspartic acid adsorption onto cryogels.
Cryogel quite preferred as synthetic materials in recent years has emerged. Adsorbents are used in the batch and continuous system operation. In this study poly(2- Hydroxyethyl methacrylate-N-methacryloyl-L-lysine), poly(HEMA-MAL), cryogels synthesized and used in adsorption from aqueous systems of L-aspartic acid. The adsorption of aspartic acid with the cryogels was tested under changing conditions of pH, interaction time, initial aspartic acid concentration, temperature, and ionic strength. For the characterization of cryogels; swelling test, Fourier Transform Infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), thermal analysis, surface area (BET), elemental analysis were performed. The adsorption capacity of cryogels was determined about 236,0 mg aspartic acid/g cryogel. Adsorption of lysozyme was studied under the optimum adsorption conditions and adsorption capacity of cryogels was found as 37,25 mg enzyme/g cryogel. The adsorption process was triad via Langmuir and Freundlich isotherm models and the Langmuir adsorption model was determined to be more appropriate for the aspartic acid adsorption onto cryogels.
Açıklama
Anahtar Kelimeler
Adsorpsiyon, Aspartik Asit, Kriyojel, Lizozim
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Sarıca, B. (2016). Aminoasit bağlı kriyojellerin sentezi ve biyolojik uygulamaları (Yüksek Lisans Tezi).
