Techniques for nucleic acid engineering: The foundation of gene manipulation

Basit Öğe Kaydı
dc.authorid0000-0001-7639-1122
dc.contributor.authorTüzmen, Şükrü
dc.contributor.authorBaskın, Yasemin
dc.contributor.authorNursal, Ayşe Feyda
dc.contributor.authorEraslan, Serpil
dc.contributor.authorEsemen, Yağmur
dc.contributor.authorÇalıbaşı, Gizem
dc.contributor.authorDemir, Ayşe Banu
dc.contributor.authorAbbasoğlu, Duygu
dc.contributor.authorHizel, Candan
dc.date.accessioned2019-05-13T09:09:07Z
dc.date.available2019-05-13T09:09:07Z
dc.date.issued2017
dc.departmentHitit Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü
dc.description.abstractThe journey of nucleic acid isolation and characterization has been a long one starting in the late 1800s. DNA isolation has led the way due to its stable chemical characteristic. Since its initial implementation the demand of molecular studies has evolved rapidly into studying a wide range of nucleic acids, including all types of RNAs, mitochondrial DNA, and nucleic acid materials of other organisms. The scientific advancements and the "omics" era certainly brought new challenges, forcing the technology to go into an enormous advancement in a relatively short period. In the early 1900s the range of methods, the material, and the field of applications were limited. During, nearly, the last three decades the procedures became automated, standardized, economic, user-friendly, nonhazardous, and less time-consuming. The manual organic or inorganic procedures had left their place to more robust and reliable solid-phase extraction techniques. There are a wide range of samples in which high-quality and high-yield nucleic acid isolation is carried out. These samples vary from whole blood to different types of tissues, hair, bones, saliva, urine, and many more. We are going to explore the principles of nucleic acid isolation, the basics of chemistry behind the scenery, manual and advanced techniques of isolation and quality check, and the means of good laboratory practice. © 2018 Elsevier Inc. All rights reserved.
dc.identifier.citationTüzmen, Ş., Baskın, Y., Nursal, A. F., Eraslan, S., Esemen, Y., Çalıbaşı, G., Demir, A. B., Abbasoğlu, D. [et.al.]. (2018). Techniques for nucleic acid engineering: The foundation of gene manipulation. D. Barh and V. Azevedo (Eds.), In Omics Technologies and Bio-Engineering (pp. 247-315). England: Academic Press.
dc.identifier.doi10.1016/B978-0-12-804659-3.00014-2
dc.identifier.endpage315en_US
dc.identifier.isbn9780128047491; 9780128046593
dc.identifier.scopusqualityN/A
dc.identifier.startpage247en_US
dc.identifier.urihttps://doi.org/10.1016/B978-0-12-804659-3.00014-2
dc.identifier.urihttps://hdl.handle.net/11491/2107
dc.identifier.volume1en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Inc.
dc.relation.ispartofOmics Technologies and Bio-engineering: Towards Improving Quality of Life
dc.relation.publicationcategoryKitap Bölümü - Uluslararası
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCesium Chlorideen_US
dc.subjectDNA Extractionen_US
dc.subjectGood Laboratory Practiceen_US
dc.subjectNucleic Acid Quantitationen_US
dc.subjectPhenol-Chloroformen_US
dc.subjectQuality Checken_US
dc.subjectRNA Extractionen_US
dc.subjectSalting Outen_US
dc.subjectSolid-Phase Purificationen_US
dc.titleTechniques for nucleic acid engineering: The foundation of gene manipulation
dc.typeBook Part

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