Magnetic nanoparticles for plasmid DNA purification through hydrophobic interaction chromatography

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dc.contributor.authorÜzek, Recep
dc.contributor.authorÖzkara, Serpil
dc.contributor.authorGüngüneş, Hakan
dc.contributor.authorUzun, Lokman
dc.contributor.authorŞenel, Serap
dc.date.accessioned2019-05-10T09:39:33Z
dc.date.available2019-05-10T09:39:33Z
dc.date.issued2014
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Fizik Bölümü
dc.description.abstractMagnetic poly(2-hydroxyethyl methacrylate-co-N-methacryloyl–L-phenyl alanine), p(HEMA-co-MAPA) nanoparticles were produced by emulsion polymerization technique. The nanoparticles were characterized by zetasizer, atomic force microscopy (AFM), transmission electron microscopy (TEM), electron spin resonance spectroscopy (ESR), and Fourier transformed infrared spectroscopy (FTIR) techniques. Experimental conditions were optimized for DNA adsorption in aqueous media. The effects of several factors (i.e., temperature, pH, equilibrium concentration of DNA, salt type, and ionic strength) on adsorption capacity were examined in batch studies. The maximum DNA adsorption capacity was 211.3 mg/g for nanoparticles consisting of magnetite. The adsorption isotherms and kinetics were also examined. The Langmuir model and pseudo second-order kinetics are the best fitted to the data. Following the ten adsorption-desorption cycles, the decrease in adsorption capacity was only 12%. Finally, plasmid DNA was purified from E.coli lysate by magnetite nanoparticles consisting of MAPA. The purity and molecular mass of purified plasmid DNA were determined by the use of agarose gel electrophoresis resulting in 7000 base pairs for molecular mass and OC pDNA form following elution.
dc.identifier.citationÜzek, R., Özkara, S., Güngüneş, H., Uzun, L., Şenel, S. (2014). Magnetic nanoparticles for plasmid DNA purification through hydrophobic interaction chromatography. Separation Science and Technology, 49(14), 2193-2203.
dc.identifier.doi10.1080/01496395.2014.905958
dc.identifier.endpage2203en_US
dc.identifier.issn0149-6395
dc.identifier.issue14en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage2193en_US
dc.identifier.urihttps://doi.org/10.1080/01496395.2014.905958
dc.identifier.urihttps://hdl.handle.net/11491/726
dc.identifier.volume49en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor and Francis Inc.
dc.relation.ispartofSeparation Science and Technology (Philadelphia)
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAdsorptionen_US
dc.subjectE. Coli Lysateen_US
dc.subjectHydrophobic Interactionen_US
dc.subjectMagnetic Nanoparticlesen_US
dc.subjectPlasmid DNAen_US
dc.titleMagnetic nanoparticles for plasmid DNA purification through hydrophobic interaction chromatography
dc.typeArticle

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