Preparation of a novel functionalized magnetic nanobiocomposite as a carrier for protein adsorption

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dc.authoridDoğan, Mustafa / 0000-0002-4437-566X
dc.authoridGüngüneş, Hakan / 0000-0002-5803-3193
dc.contributor.authorMetin, Ayşegül Ülkü
dc.contributor.authorDoğan, Mustafa
dc.contributor.authorErdem, Ümit
dc.contributor.authorBabacan, Taner
dc.contributor.authorGüngüneş, Hakan
dc.date.accessioned2021-11-01T15:05:03Z
dc.date.available2021-11-01T15:05:03Z
dc.date.issued2020
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Fizik Bölümü
dc.description.abstractThis study aims the synthesis of a novel functionalized magnetic nanocarrier based on xanthan gum biopolymer. Glycidyl methacrylate was grafted on xanthan gum chains by radical polymerization reaction using two types of initiators: ammonium persulfate and benzoyl peroxide. Characterization studies of the magnetic nanocarrier were performed using several instruments such as Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, Energy-Dispersive-X-Ray Spectroscopy, X-Ray Diffraction Spectroscopy, Transmission Electron Microscopy, Mossbauer Spectroscopy, and Vibrating Sample Magnetometer. According to the Vibrating Sample Magnetometer data and Mossbauer analysis, Fe atoms were incorporated successfully in the polymer chains in Fe3+ state and magnetic nanocarrier has super-paramagnetic behavior, respectively. Epoxy groups on magnetic nanoparticles were converted to carboxylic acid groups using iminodiacetic acid and then tested to usability as a carrier for immobilization of proteins such as albumin, lipase and cytochrome c. The adsorption of albumin and lipase on magnetic nanoparticles were pH-dependent while cytochrome c was immobilized in a wide range of pH value. The calculated maximum experimental immobilization capacity of magnetic nanoparticles was 65.10 mg g(-1), 62.0 mg g(-1) and 188.0 mg g(-1) for albumin, lipase and cytochrome c, respectively. Experimental data fitted to Langmuir isotherm better than Freundlich. The rate of cytochrome c adsorption followed the pseudo-second-order kinetic. Results showed that the functionalized magnetic nanoparticles can be effectively used as a carrier for protein separations, especially for cytochrome c. Moreover, the functionalized magnetic nanocarrier had high affinity to Cytochrome c protein even in multiple protein systems.
dc.identifier.citationMetin, A. Ü., Doğan, M., Erdem, Ü., Babacan, T., & Güngüneş, H. (2020). Preparation of a novel functionalized magnetic nanobiocomposite as a carrier for protein adsorption. Spectroscopy Letters, 53(4), 289-305.
dc.identifier.doi10.1080/00387010.2020.1737945
dc.identifier.endpage305en_US
dc.identifier.issn0038-7010
dc.identifier.issn1532-2289
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85082797441
dc.identifier.scopusqualityQ3
dc.identifier.startpage289en_US
dc.identifier.urihttps://doi.org/10.1080/00387010.2020.1737945
dc.identifier.urihttps://hdl.handle.net/11491/7068
dc.identifier.volume53en_US
dc.identifier.wosWOS:000523153600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorGüngüneş, Hakan
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofSpectroscopy Letters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCarboxylic Acid Groupsen_US
dc.subjectGlycidyl Methacrylateen_US
dc.subjectIron Oxideen_US
dc.subjectProteinsen_US
dc.subjectXanthan Gumen_US
dc.titlePreparation of a novel functionalized magnetic nanobiocomposite as a carrier for protein adsorption
dc.typeArticle

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