Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines

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dc.contributor.authorMesci, Seda
dc.contributor.authorGül, Melek
dc.contributor.authorYıldırım, Tuba
dc.date.accessioned2021-11-01T15:05:38Z
dc.date.available2021-11-01T15:05:38Z
dc.date.issued2021
dc.departmentHitit Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractObjective: To investigate the antioxidant and cytotoxic activities of sulfur-containing glycine imine derivatives MCF-7 (human breast adenocarcinoma) and DLD-1 (colorectal adenocarcinoma) cell lines. Methods: This study examined the antioxidant activities (25-200 mu M) of sulfur-containing glycine imine derivatives via the DPPH, metal chelating and reduction methods. Furthermore the cytotoxic activity of MCF-7, MCF-12A (normal breast epithelial) and DLD-1, CCD-18CO (normal colon fibroblast) were examined with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and RTCA (Real-time Cell Analysis) assays. Results: The antioxidant assay of the metal chelating activity showed significant results (71, 77 and 40% respectively) as compared to knowing synthetic antioxidant (trolox; 95.45, EDTA; 97.06 %). Reducing activity was found to be very low compared to the standard compounds. Compounds were shown to be moderated by DPPH (2,2-Diphenyl-1-picrylhydrazyl) activity, and the IC50 value ranged from 91 to 150. The IC50 values (100 mu M) of the MTT and RTCA analyses were similar. Conclusion: The study showed that the compounds had selective and significant antioxidant activities, and we also found that they had cytotoxic effects on MCF-7 and DLD-1 cells.
dc.identifier.citationMesci, S., Melek, G. Ü. L., & Yildirim, T. (2021). Antioxidant and cytotoxic activity studies of sulfur containing glycine imine derivatives MCF-7 and DLD-1 cell lines. Clinical and Experimental Health Sciences, 11(1), 81-90.
dc.identifier.doi10.33808/clinexphealthsci.787954
dc.identifier.endpage90en_US
dc.identifier.issn2459-1459
dc.identifier.issue1en_US
dc.identifier.startpage81en_US
dc.identifier.urihttps://doi.org/10.33808/clinexphealthsci.787954
dc.identifier.urihttps://hdl.handle.net/11491/7355
dc.identifier.volume11en_US
dc.identifier.wosWOS:000636479400013
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.institutionauthorMesci, Seda
dc.language.isoen
dc.publisherMarmara Univ, Inst Health Sciences
dc.relation.ispartofClinical And Experimental Health Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSulfuren_US
dc.subjectGlycine Imineen_US
dc.subjectMTTen_US
dc.subjectRTCAen_US
dc.subjectDPPHen_US
dc.subjectMetal chelatingen_US
dc.titleAntioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines
dc.typeArticle

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