A novel RP-LC method for determination of pK a values of some anticancer agents and their assay

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dc.contributor.authorŞanlı, Senem
dc.contributor.authorAkmeşe, Bediha
dc.contributor.authorŞanlı, Nurullah
dc.contributor.authorÖzkan, Sibel Ayşıl
dc.date.accessioned2019-05-10T09:38:50Z
dc.date.available2019-05-10T09:38:50Z
dc.date.issued2013
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractA simple, reliable, and rapid RP-LC method has been developed for the determination of some anticancer drugs (daunorubicin, doxorubicin and vincristine sulfate) in their dosage forms and human urine. These compounds are well separated on a C18 column using the mobile phase consisting of a mixture of acetonitrile (50:50; v/v) at a flow rate of 1.5 mL min -1 . The analyte peaks were detected at 235 nm for doxorubicin and daunorubicin, and 220 nm for vincristine. Linearity was obtained in different concentration ranges between 0.10 and 12 ?g mL -1 for all compounds. Good sensitivity for all analytes was observed with DAD detection. LOD and LOQ of the method were found satisfying. The proposed method has been extensively validated in accordance with ICH guidelines and obtained results proved that the proposed method was precise, accurate, selective, and sensitive for simultaneous analysis of studied compounds. All analytical procedures including sample preparation, flow rate, and run time were at low levels. Also, pK a values were determined using the dependence of the retention factor on the pH of the mobile phase. The effect of the mobile phase composition on the ionization constant was studied by measuring the pK a at different methanol-water mixtures, ranging between 45 and 60 % (v/v). © 2013 Springer-Verlag Berlin Heidelberg.
dc.identifier.citationSanli, S., Akmese, B., Sanli, N., Ozkan, S. A. (2013). A novel RP-LC method for determination of pK a values of some anticancer agents and their assay. Chromatographia, 76(21-22), 1467-1475.
dc.identifier.doi10.1007/s10337-013-2450-6
dc.identifier.endpage1475en_US
dc.identifier.issn0009-5893
dc.identifier.issue21-22en_US
dc.identifier.scopusqualityQ3
dc.identifier.startpage1467en_US
dc.identifier.urihttps://doi.org/10.1007/s10337-013-2450-6
dc.identifier.urihttps://hdl.handle.net/11491/499
dc.identifier.volume76en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofChromatographia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectColumn Liquid Chromatographyen_US
dc.subjectDaunorubicinen_US
dc.subjectDoxorubicinen_US
dc.subjectPK Aen_US
dc.subjectVincristine Sulfateen_US
dc.titleA novel RP-LC method for determination of pK a values of some anticancer agents and their assay
dc.typeArticle

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