Optimization of the experimental conditions for macrolide antibiotics in high performance liquid chromatography by using response surface methodology and determination of tylosin in milk samples

Basit Öğe Kaydı
dc.contributor.authorŞanlı, Senem
dc.contributor.authorPalabıyık, İsmail M.
dc.contributor.authorŞanlı, Nurullah
dc.contributor.authorGüzel Seydim, Zeynep B.
dc.contributor.authorAlsancak, Güleren
dc.date.accessioned2019-05-13T08:59:01Z
dc.date.available2019-05-13T08:59:01Z
dc.date.issued2011
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractThe simple, rapid and sensitive liquid chromatographic separation of five macrolides (tilmicosin, erythromycin, tylosin, roxithromycin and josamycin) widely used in food producing animals was developed. Response surface methodology was used as an optimization method of mobile phase, column temperature and pH to provide the best resolution of these analytes. The separation was performed by using an end-capped X-Terra RP-18 column (250 × 4.6 mm I.D × 5 m) with an isocratic system of 15 mM hydrochloric acid (pH 2.5)-acetonitrile as the mobile phase at a temperature of 30°C and flow-rate of 1.0 mL/min. The suitability of the method for multi-residue determination of the macrolides is demonstrated by the analysis of milk samples spiked with tylosin. Roxithromycin was used as internal standard. The recovery of tylosin was quite good as 90.8%. The limit of quantification and detection limit were 0.024 and 0.007 ?g/mL, respectively. The method was successfully applied to determination of macrolides at levels below the maximum concentration legally allowed in milk samples. © 2011 Pleiades Publishing, Ltd.
dc.identifier.citationŞanlı, S., Palabıyık, İ. M., Şanlı, N., Güzel Seydim, Z. B., Alsancak, G. (2011). Optimization of the experimental conditions for macrolide antibiotics in high performance liquid chromatography by using response surface methodology and determination of tylosin in milk samples. Journal of Analytical Chemistry, 66(9), 838-847.
dc.identifier.doi10.1134/S1061934811090152
dc.identifier.endpage847en_US
dc.identifier.issn1061-9348
dc.identifier.issue9en_US
dc.identifier.scopusqualityQ3
dc.identifier.startpage838en_US
dc.identifier.urihttps://doi.org/10.1134/S1061934811090152
dc.identifier.urihttps://hdl.handle.net/11491/1238
dc.identifier.volume66en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofJournal of Analytical Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntibiotic Residuesen_US
dc.subjectLiquid Chromatographyen_US
dc.subjectMacrolidesen_US
dc.subjectMilken_US
dc.subjectResponse Surface Methodologyen_US
dc.titleOptimization of the experimental conditions for macrolide antibiotics in high performance liquid chromatography by using response surface methodology and determination of tylosin in milk samples
dc.typeArticle

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu