Wet electrospun silk fibroin/gold nanoparticle 3D matrices for wound healing applications

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dc.contributor.authorAktürk, Ömer
dc.contributor.authorKısmet, Kemal
dc.contributor.authorYastı, Ahmet Çınar
dc.contributor.authorKuru, Serdar
dc.contributor.authorDuymuş, Mehmet E.
dc.contributor.authorKaya, Feridun
dc.contributor.authorÇaydere, Muzaffer
dc.contributor.authorHücümenoğlu, Sema
dc.contributor.authorKeskin, Dilek
dc.date.accessioned2019-05-13T09:07:27Z
dc.date.available2019-05-13T09:07:27Z
dc.date.issued2016
dc.departmentHitit Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü
dc.description.abstractThis study aimed to fabricate 3D silk fibroin (SF) matrices for skin tissue engineering applications. SF/poly(ethylene oxide) solutions were wet electrospun to obtain a fibrous network (0.7–20 ?m diameter), which were then lyophilized to obtain 3D porous nanofibrous matrices (SFM-E: ethanol treated silk fibroin matrices). SF matrices were loaded with citrate-capped gold nanoparticles (AuNPs, 14.27 ppm, Daverage = 24 nm) (SFM-AuE: ethanol treated silk fibroin matrices incorporated with AuNPs) and investigated for structural and chemical properties, in vitro biocompatibility and in vivo full-thickness dermal wound healing efficacy in a rat model. AuNP incorporation enhanced the degradation profiles and mechanical properties significantly. SFM-E and SFM-AuE showed similar cell attachment and layer by layer proliferation behaviour, but cells had more spread and flattened morphology on SFM-AuE. Both matrix extracts had high cell viability (>90%), indicating good in vitro biocompatibility. Wound closure was statistically more than the untreated skin control (UTSC) in SFM-E and SFM-AuE applied groups. The recovered tensile strength and elastic modulus of SFM-E and SFM-AuE (40–60%) were not as high as the unwounded skin control (UWSC), but they had elongation at break values similar to UWSC. This was attributed to the still ongoing medium to high inflammation levels leading to a low and immature extent of collagen fibrils on postoperative 14th day. There was only a small amount of epithelialization due to scab formation and medium to high level inflammation for both SFM-E and SFM-AuE, but they were better than UTSC in terms of neovascularization and granulation tissue formation. As a whole, inclusion of AuNPs to SF matrices at 14.27 ppm loading brought some enhancement in the matrix properties and did not cause any toxicity in in vitro and in vivo conditions and even had potency to promote wound healing stages.
dc.identifier.citationAktürk, Ö., Kısmet, K., Yastı, A. Ç., Kuru, S., Duymuş, M. E., Kaya, F., Caydere, M., Hücümenoğlu, S. [et.al.].(2016). Wet electrospun silk fibroin/gold nanoparticle 3D matrices for wound healing applications. RSC Advances, 6(16), 13234-13250.
dc.identifier.doi10.1039/c5ra24225h
dc.identifier.endpage13250en_US
dc.identifier.issn2046-2069
dc.identifier.issue16en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage13234en_US
dc.identifier.urihttps://doi.org/10.1039/c5ra24225h
dc.identifier.urihttps://hdl.handle.net/11491/1803
dc.identifier.volume6en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofRSC Advances
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject[Belirlenecek]en_US
dc.titleWet electrospun silk fibroin/gold nanoparticle 3D matrices for wound healing applications
dc.typeArticle

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