Determination of pKa values of some antihypertensive drugs by liquid chromatography and simultaneous assay of lercanidipine and enalapril in their binary mixtures

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dc.authorid0000-0003-2793-7154
dc.authorid0000-0001-7494-3077
dc.contributor.authorGümüştaş, Mehmet
dc.contributor.authorŞanlı, Senem
dc.contributor.authorŞanlı, Nurullah
dc.contributor.authorÖzkan, Sibel Ayşıl
dc.date.accessioned2019-05-10T09:39:18Z
dc.date.available2019-05-10T09:39:18Z
dc.date.issued2010
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractIn this study, pKa values were determined using the dependence of the retention factor on the pH of the mobile phase for three ionizable substances, namely, enalapril, lercanidipine and ramipril (IS). The effect of the mobile phase composition on the ionization constant was studied by measuring the pKa at different methanol-water mixtures, ranging between 50 and 65% (v/v), using LC-DAD method. Two simple, accurate, precise and fully validated analytical methods for the simultaneous determination of enalapril and lercanidipine in combined dosage forms have been developed. Separation was performed on an X-Terra RP-18 column (250 mm × 4.60 mm ID × 5 ?m) at 40 °C with the mobile phase of methanol-water 55:45 (v/v) adjusted to pH 2.7 with 15 mM orthophosphoric acid. Isocratic elution was performed in less than 12 min with a flow rate of 1.2 mL min-1. Good sensitivity for the analytes was observed with DAD detection. The LC method allowed quantitation over the 0.50-20.00 ?g mL-1 range for enalapril and lercanidipine. The second method depends on first derivative of the ratio-spectra by measurements of the amplitudes at 219.7 nm for enalapril and 233.0 nm for lercanidipine. Calibration graphs were established for 1-20 ?g mL-1 for enalapril and 1-16 ?g mL-1 lercanidipine, using first derivative of the ratio spectrophotometric method. Both methods have been extensively validated. These methods allow a number of cost and time saving benefits. The described methods can be readily utilized for analysis of pharmaceutical formulations. The methods have been applied, without any interference from excipients, for the simultaneous determination of these compounds in tablets. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. © 2010 Elsevier B.V. All rights reserved.
dc.identifier.citationGumustas, M., Şanlı, S., Şanlı, N., & Ozkan, S. A. (2010). Determination of pKa values of some antihypertensive drugs by liquid chromatography and simultaneous assay of lercanidipine and enalapril in their binary mixtures. Talanta, 82(4), 1528-1537.
dc.identifier.doi10.1016/j.talanta.2010.07.037
dc.identifier.endpage1537en_US
dc.identifier.issn0039-9140
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage1528en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2010.07.037
dc.identifier.urihttps://hdl.handle.net/11491/661
dc.identifier.volume82en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDeterminationen_US
dc.subjectEnalaprilen_US
dc.subjectLercanidipineen_US
dc.subjectLiquid Chromatographyen_US
dc.subjectRamiprilen_US
dc.titleDetermination of pKa values of some antihypertensive drugs by liquid chromatography and simultaneous assay of lercanidipine and enalapril in their binary mixtures
dc.typeArticle

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