The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method

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dc.contributor.authorAkmeşe, Bediha
dc.contributor.authorBakırhan, Nurgül Karadaş
dc.contributor.authorGöver, Tuğçe
dc.contributor.authorÖzkan, Sibel A.
dc.date.accessioned2021-11-01T18:14:54Z
dc.date.available2021-11-01T18:14:54Z
dc.date.issued2019
dc.department[Belirlenecek]
dc.description.abstractDaptomycin is known as the first lipopeptide antibiotic shows activity against gram-positive bacteria. A fast, simple and repeatabile RP-LC method has been developed for the determination of daptomycin in mobile phase solution and human urine. This drug was exposed to various stress conditions such as hydrolytic, oxidative, thermal and photolytic and the stressed samples were detected by the proposed method. Daptomycin was well separated on a X-Terra RP-18 column using the mobile phase consisting of a mixture of acetonitrile:water (45:55 v/v) at a flow rate of 1 mL min-1. The system operates at 222 nm for daptomycin and 319 nm and tinidazole was used as internal standard under these conditions. The linearity of daptomycin was obtained between 0.50 and 12 µg mL-1 concentration range. Limits of detection was determined as 0.1176 and 0.1412 µg mL-1 and the limits of quantification was obtained as 0.3563 and 0.4277 µg mL-1 in the mobile phase and urine, respectively. Hydrochloric acid, sodium hydroxide, hydrogen peroxide affected response of daptomycin under stress degradation conditions.
dc.identifier.doi10.12991/jrp.2019.122
dc.identifier.endpage176en_US
dc.identifier.issn2630-6344
dc.identifier.issue2en_US
dc.identifier.startpage170en_US
dc.identifier.urihttps://doi.org10.12991/jrp.2019.122
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpNM05URTBOQT09
dc.identifier.urihttps://hdl.handle.net/11491/7569
dc.identifier.volume23en_US
dc.identifier.wosWOS:000459615300003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.institutionauthor[Belirlenecek]
dc.language.isoen
dc.relation.ispartofJournal of research in pharmacy (online)
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFarmakoloji ve Eczacılıken_US
dc.titleThe development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method
dc.typeArticle

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