Phylogenetic analysis and prevalence of human papillomavirus (HPV) in women with several cervical pathologies

Basit Öğe Kaydı
dc.authorid0000-0003-4049-0756
dc.contributor.authorAlp Avcı, Gülçin
dc.contributor.authorBozdayı, Gülendam
dc.contributor.authorTaşkıran, Çağatay
dc.contributor.authorÖzkan, Seçil
dc.contributor.authorOnan, Mehmet Anıl
dc.date.accessioned2019-05-13T09:08:40Z
dc.date.available2019-05-13T09:08:40Z
dc.date.issued2013
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü
dc.description.abstractObjective: To determinate the prevalence of HPV types in patients with cervical cancers in our legion by Real time PCR and DNA sequence analysis and to make phylogenetic analysis was aimed in this study. Material and methods: From January to October 2010, cervical swap samples of 77 patients directed to colposcopy were included in the study. HPV DNA and HPV type 16 were detected by Real Time polymerase chain reaction using the L1 region. Real Time PCR amplifications of MY09/11 products were done by GP5+/GP6+ primers and Cyanine-5 labeled HPV DNA and HPV type 16 specific probe. HPV types determinate by GP5+/GP6+. Phylogenetic analysis of sequences was calculated by Kimura's two parameters method. Statistically analyses were by using Pearson chisquare and odss ratio tests. Results: Forty seven samples (prevalence; 61%) of total seventy seven cervical samples detected as HPV DNA positive. While HPV type 16; 52%, HPV type 16+11; 4%, HPV type 16+6; 1% and non-typing HPV DNA 4% of seventy seven samples determining, 39% of samples observed as negative HPV. Participated in the study population, HPV DNA positive individuals are among 34-56 years. Most HPV DNA positivity rate of 80.0% was between the ages of 31-40. 52.2% of HPV DNA positivity between the ages of 41-50 to fall, but again, 83.3% between the ages of 51-60 to a second peak was determined that increased. 60.0% of 20 ASC-H cases, 63.8% of 36 ASC-US cases, 100% 9 of HSIL cases and 25.0% of 12 LSIL cases were positive for HPV DNA. Conclusion: The investigation of the distribution of HPV genotypes in women with cervical cancer and precancerous lesions in our region is important. Early diagnosis of HPV by using improved technological assays, play a key role to prevent the turn precancerous lesions into invasive cancers.
dc.identifier.citationAlp Avcı, G., Bozdayı, G., Taşkıran, Ç., Özkan, S., Onan, M. A. Phylogenetic analysis and prevalence of human papillomavirus (HPV) in women with several cervical pathologies. Journal of Turkish Society of Obstetrics and Gynecology, 10(3), 151-159.
dc.identifier.doi10.5505/tjod.2013.03779
dc.identifier.endpage159en_US
dc.identifier.issn1307-699X
dc.identifier.issue3en_US
dc.identifier.scopusqualityN/A
dc.identifier.startpage151en_US
dc.identifier.urihttps://doi.org/10.5505/tjod.2013.03779
dc.identifier.urihttps://hdl.handle.net/11491/2031
dc.identifier.volume10en_US
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTurkish Society of Obstetrics and Gynecology
dc.relation.ispartofJournal of Turkish Society of Obstetrics and Gynecology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCancer of Cervixen_US
dc.subjectDNA Sequence Analysisen_US
dc.subjectHuman Papillomavirusen_US
dc.subjectPhylogenetic Analysisen_US
dc.subjectReal Time PCRen_US
dc.subjectDNA Dizi Analizien_US
dc.subjectFilogenetik Analizen_US
dc.subjectGerçek Zamanlı PCRen_US
dc.subjectİnsan Papillomavirusen_US
dc.subjectServiks Kanserien_US
dc.titlePhylogenetic analysis and prevalence of human papillomavirus (HPV) in women with several cervical pathologies
dc.title.alternativeÇeşitli servikal patolojiye sahip kadınlarda human papillomavirus (HPV) prevalansı ve filogenetik analizien_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Avcı, Gülçin Alp.pdf
Boyut:
744.39 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu