Immobilization of Aspergillus oryzae ?-galactosidase on low-pressure plasma-modified cellulose acetate membrane using polyethyleneimine for production of galactooligosaccharide
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Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study was to produce galactooligosaccharides (GOS) from lactose using ?-galactosidase from Aspergillus oryzae immobilized on a low-pressure plasma-modified cellulose acetate (CA) membrane. Specifically, a novel method was developed for multilayer enzyme immobilization involving polyethyleneimine (PEI)-enzyme aggregate formation and growth on a CA membrane. A large amount of enzyme (997 ?g/cm2 membrane) was immobilized with 66% efficiency. The Km value for the immobilized enzyme was estimated to be 48 mM, which indicates decreased affinity for the substrate, whereas the Vmax value was smaller. The immobilized enzyme showed good storage and operational stability. The half-life of the immobilized enzyme on the membrane was about 1 month at 30°C and ? 60 h at 60°C. Maximum GOS production of 27% (w/w) was achieved with 70% lactose conversion from 320 g/L of lactose at pH 4.5 and 60°C. Trisaccharides were the major types of GOS formed and accounted for about 75% of the total GOS produced. Based on these results, immobilized enzyme technology could be applied to GOS production from lactose. © 2010 The Korean Society for Biotechnology and Bioengineering and Springer-Verlag.
Açıklama
Anahtar Kelimeler
Cellulose Acetate Membrane, Enzyme Immobilization, Galactooligosaccharide, Low-Pressure Plasma Polymerization, Polyethyleneimine
Kaynak
Biotechnology and Bioprocess Engineering
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
15
Sayı
6
Künye
Güleç, H. A., Gürdaş, S., Albayrak, N., Mutlu, M. (2010). Immobilization of Aspergillus oryzae ?-galactosidase on low-pressure plasma-modified cellulose acetate membrane using polyethyleneimine for production of galactooligosaccharide. Biotechnology and Bioprocess Engineering, 15(6), 1006-1015.
