Immobilization of Aspergillus oryzae ?-galactosidase on low-pressure plasma-modified cellulose acetate membrane using polyethyleneimine for production of galactooligosaccharide

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dc.contributor.authorGüleç, Hacı Ali
dc.contributor.authorGürdaş, Sevim
dc.contributor.authorAlbayrak, Nedim
dc.contributor.authorMutlu, Mehmet
dc.date.accessioned2019-05-13T09:02:39Z
dc.date.available2019-05-13T09:02:39Z
dc.date.issued2010
dc.departmentHitit Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü
dc.description.abstractThe aim of this study was to produce galactooligosaccharides (GOS) from lactose using ?-galactosidase from Aspergillus oryzae immobilized on a low-pressure plasma-modified cellulose acetate (CA) membrane. Specifically, a novel method was developed for multilayer enzyme immobilization involving polyethyleneimine (PEI)-enzyme aggregate formation and growth on a CA membrane. A large amount of enzyme (997 ?g/cm2 membrane) was immobilized with 66% efficiency. The Km value for the immobilized enzyme was estimated to be 48 mM, which indicates decreased affinity for the substrate, whereas the Vmax value was smaller. The immobilized enzyme showed good storage and operational stability. The half-life of the immobilized enzyme on the membrane was about 1 month at 30°C and ? 60 h at 60°C. Maximum GOS production of 27% (w/w) was achieved with 70% lactose conversion from 320 g/L of lactose at pH 4.5 and 60°C. Trisaccharides were the major types of GOS formed and accounted for about 75% of the total GOS produced. Based on these results, immobilized enzyme technology could be applied to GOS production from lactose. © 2010 The Korean Society for Biotechnology and Bioengineering and Springer-Verlag.
dc.identifier.citationGüleç, H. A., Gürdaş, S., Albayrak, N., Mutlu, M. (2010). Immobilization of Aspergillus oryzae ?-galactosidase on low-pressure plasma-modified cellulose acetate membrane using polyethyleneimine for production of galactooligosaccharide. Biotechnology and Bioprocess Engineering, 15(6), 1006-1015.
dc.identifier.doi10.1007/s12257-010-0046-7
dc.identifier.endpage1015en_US
dc.identifier.issn1226-8372
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage1006en_US
dc.identifier.urihttps://doi.org/10.1007/s12257-010-0046-7
dc.identifier.urihttps://hdl.handle.net/11491/1298
dc.identifier.volume15en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofBiotechnology and Bioprocess Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCellulose Acetate Membraneen_US
dc.subjectEnzyme Immobilizationen_US
dc.subjectGalactooligosaccharideen_US
dc.subjectLow-Pressure Plasma Polymerizationen_US
dc.subjectPolyethyleneimineen_US
dc.titleImmobilization of Aspergillus oryzae ?-galactosidase on low-pressure plasma-modified cellulose acetate membrane using polyethyleneimine for production of galactooligosaccharide
dc.typeArticle

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