Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori

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dc.contributor.authorCelik, Cagla
dc.contributor.authorSezgin, Gulten Can
dc.contributor.authorKocabas, Umut Gorkem
dc.contributor.authorGursoy, Sebnem
dc.contributor.authorIldiz, Nilay
dc.contributor.authorTan, Weihong
dc.contributor.authorOcsoy, Ismail
dc.date.accessioned2021-11-01T15:06:08Z
dc.date.available2021-11-01T15:06:08Z
dc.date.issued2021
dc.department[Belirlenecek]
dc.description.abstractSeveral different diagnostic tests have been reported for rapid, sensitive, and economical detection of bacterial pathogens, but most lack widespread and practical use in the clinic. In this study, we used anthocyanins from red cabbage (Brassica oleracea) as a natural pH indicator and, for the first time, incorporated this agent into a simple, rapid, and economical colorimetric strategy for the detection of Helicobacter pylori (H. pylori) (RCE@test). We prepared two sets of RCE@test solutions (test 1 is purple, and test 2 is blue) in different forms, including liquid, adsorbed filter paper, and agar, and investigated the performance of each RCE@test as a function of the test volume, H. pylori concentration, and reaction time. To elucidate the effect of the pathophysiological environment on these RCE@tests, H. pylori in an artificial gastric fluid was also detected. The 10 and 1 CFU/mL H. pylori suspensions were detected in 15 min and 3 h, respectively, and the limit of detection was determined down to 1 CFU/mL. We experimentally demonstrated the advantages of the RCE@test for detection of H. pylori by comparing it to a commercially available rapid urease test, the CLO test (Campylobacter-like organism test). In addition to colorimetric detection by the naked eyes, RGB (Red Green Blue) and Delta-E analysis in image-processing software was run to quantitatively monitor changes of color in the RCE@test using a smartphone application. Finally, we propose that this test provides simple, effective, rapid, and inexpensive detection and that it can be easily implemented for clinical use.
dc.description.sponsorship2020 BAGEP Award of the Science Academy; Turkish Academy of Sciences (2018 TUBA GEBIP Award)en_US
dc.description.sponsorshipThis work was supported by the 2020 BAGEP Award of the Science Academy and Turkish Academy of Sciences (2018 TUBA GEBIP Award). We would like to thank Mucahit Osman Turkan and Busra Aydin for their assistance in drawing and designing TOC.en_US
dc.identifier.doi10.1021/acs.analchem.1c00663
dc.identifier.endpage6253en_US
dc.identifier.issn0003-2700
dc.identifier.issn1520-6882
dc.identifier.issue15en_US
dc.identifier.pmid33825433
dc.identifier.scopus2-s2.0-85104943598
dc.identifier.scopusqualityQ1
dc.identifier.startpage6246en_US
dc.identifier.urihttps://doi.org/10.1021/acs.analchem.1c00663
dc.identifier.urihttps://hdl.handle.net/11491/7497
dc.identifier.volume93en_US
dc.identifier.wosWOS:000643539400033
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[Belirlenecek]
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAnalytical Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject[No Keywords]en_US
dc.titleNovel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori
dc.typeArticle

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