Electrochemical Determination and In Silico Studies of Fludarabine on NH2 Functionalized Multiwalled Carbon Nanotube Modified Glassy Carbon Electrode
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Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A sensitive voltammetric technique has been developed for the determination of Fludarabine using amine-functionalized multi walled carbon nanotubes modified glassy carbon electrode (NH2-MWCNTs/GCE). Molecular dynamics simulations, an in silico technique, were employed to examine the properties including chemical differences of Fludarabine- functionalized MWCNT complexes. The redox behavior of Fludarabine was examined by cyclic, differential pulse and square wave voltammetry in a wide pH range. Cyclic voltammetric investigations emphasized that Fludarabine is irreversibly oxidized at the NH2-MWCNTs/GCE. The electrochemical behavior of Fludarabine was also studied by cyclic voltammetry to evaluate both the kinetic (k(s) and E-a) and thermodynamic (Delta H, Delta G and Delta S) parameters on NH2-MWCNTs/GCE at several temperatures. The mixed diffusion-adsorption controlled electrochemical oxidation of Fludarabine revealed by studies at different scan rates. The experimental parameters, such as pulse amplitude, frequency, deposition potential optimized for square-wave voltammetry. Under optimum conditions in phosphate buffer (pH 2.0), a linear calibration curve was obtained in the range of 2x10(-7) M-4x10(-6) M solution using adsorptive stripping square wave voltammetry. The limit of detection and limit of quantification were calculated 2.9x10(-8) M and 9.68x10(-8) M, respectively. The developed method was applied to the simple and rapid determination of Fludarabine from pharmaceutical formulations.
Açıklama
Anahtar Kelimeler
Fludarabine, nanosensor, multi walled carbon nanotubes, thermodynamic, molecular dynamics
Kaynak
Electroanalysis
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
32
Sayı
1
