Isolation, phylogenetic analysis and antimicrobial activity of halophilic actinomycetes from different saline environments located near Çorum province

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dc.authoridTatar, Demet / 0000-0002-9317-3263
dc.authorwosidTatar, Demet / AAY-4486-2021
dc.authorwosidTatar, Demet / AAY-4347-2021
dc.contributor.authorTatar, Demet
dc.date.accessioned2021-11-01T15:05:26Z
dc.date.available2021-11-01T15:05:26Z
dc.date.issued2021
dc.departmentHitit Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü
dc.description.abstractThe aim of this study was to isolate and characterize halophilic actinomycetes. Halophilic actinomycetes isolates were obtained from soil samples from Iskilip-Kekec, Tayrak, Yerlikoy and Sungurlu-Akpinar, Akcakoyunlu, Alibaba, Boncuk salterns located near corum province with a dilution plate technique on four selective media with 3%, 10% and 15% NaCl. PCR amplification belonging to 16S rRNA gene region of the isolates was realized out with primers of 27F and 1525R. Phylogenetic trees were formed with the three different algorithms in MEGA 7.0 software. All fifteen microorganisms were isolated on four selective media with different salt proportions and based on 16S rRNA gene sequence analysis of 15 isolates, eleven Streptomyces isolates, two Nocardiopsis isolates, two Saccharopolyspora isolates were determined. 7 of the strains exhibited only antibacterial activity, 3 only antifungal activity, and 1 both antibacterial and antifungal activities In addition, NRPS, PKS-I ve PKS-II gene regions of isolates with antimicrobial activity were investigated with related primers. According to the results of the work done, of the 11 actinomycete isolates, 4 were found to have PKS-I, 5 were PKS-II and 8 had NRPS gene regions. When the results were evaluated, it was observed that the isolates contained more nonribozomal peptide synthetase (NRPS) gene clusters than type I-II polyketide synthase (PKS-I-II) gene clusters. In addition, it was determined that 1 isolate contain three gene clusters together. As a result, it was found that actinomycetes isolated from saline soils have the capacity to produce secondary metabolites.
dc.description.sponsorshipHitit University Scientific Research Projects UnitHitit University [ODMYO19001.17.001]en_US
dc.description.sponsorshipThis study was supported by Hitit University Scientific Research Projects Unit (ODMYO19001.17.001).en_US
dc.identifier.citationTatar, D. (2021). Isolation, phylogenetic analysis and antimicrobial activity of halophilic actinomycetes from different saline environments located near Çorum Province. Biologia, 76(2), 773-780.
dc.identifier.doi10.2478/s11756-020-00612-w
dc.identifier.endpage780en_US
dc.identifier.issn0006-3088
dc.identifier.issn1336-9563
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85093955242
dc.identifier.scopusqualityQ2
dc.identifier.startpage773en_US
dc.identifier.urihttps://doi.org/10.2478/s11756-020-00612-w
dc.identifier.urihttps://hdl.handle.net/11491/7273
dc.identifier.volume76en_US
dc.identifier.wosWOS:000582087500001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorTatar, Demet
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofBiologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHalophilic actinomycetesen_US
dc.subject16S rRNA geneen_US
dc.subjectAntimicrobial activityen_US
dc.subjectNRPSen_US
dc.subjectPKS-I and PKS-II genesen_US
dc.titleIsolation, phylogenetic analysis and antimicrobial activity of halophilic actinomycetes from different saline environments located near Çorum province
dc.typeArticle

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